Applied Biological Materials

abm's Illumina certified RNA-seq service produces high quality strand-specific sequencing data from low sample input and includes complementary bioinformatics analysis to accurately profile gene expression levels to facilitate your next big discovery.

Key features:

• Starting material from 100ng of total RNA, poly-A enriched mRNA, or rRNA depleted RNA
• Strand specific single-end or paired-end sequencing
• Complementary bioinformatics analysis.
• Fast turn-around time (2-3 weeks)

Now included in RNA-Seq service:

• Sample Enrichment (PolyA or miRNA enrichment as required).
• Library Preparation.
• QC (RSeQC/FastQC).
• Basic Bioinformatics Analyses
• Industry standard output in FASTQ format
• Standard exploratory bioinformatics analysis
• Read mapping
• Relative gene expression level estimation
• 3 months FREE storage of raw data

Applications:

• Gene expression profile
• de novo transcript assembly
• Detection of novel isoforms and splice junctions
• Inter-species variation detection

Deliverables:

1. Sequencing results (FASTQ)
2. Expression level estimations in FPKM and TPM
3. Differentially expressed genes (If applicable)
4. QC and full analysis report

Single-End Sequencing Services:

• Total RNA Sequencing (8 million reads, 1x75bp SE)
• Total RNA Sequencing (20 million reads, 1x75bp SE)
• Total RNA Sequencing (40 million reads, 1x75bp SE)
• Total RNA Sequencing (80 million reads, 1x75bp SE)
• miRNA/Small RNA Sequencing (10 million, 1x75bp SE)

Paired-End Sequencing Services:

• Total RNA Sequencing (8 million reads, 2x75bp PE)
• Total RNA Sequencing (20 million reads, 2x75bp PE)
• Total RNA Sequencing (40 Million Reads, 2x75bp PE)
• Total RNA Sequencing (80 Million Reads, 2x75bp PE)

Additional Bioinformatics Services:

• Pairwise Comparison for mRNA
• Pairwise Comparison for miRNA
• Functional annotation of differentially expressed genes (GO, KEGG, OMIM, PFAM)
• SNP Detection
• Novel Splice Junction Detection
• Novel Isoform Discovery
• Custom Bioinformatics analysis

Reference Genome Import:

• Reference Genome Import for Human or Mouse
• Reference Genome Import (<10Mb genome)
• Reference Genome Import (>10Mb genome)

Additional Sample Preparation Services:

• rRNA Depletion Treatment (human, mouse, or rat)
• rRNA Depletion Treatment (plant or bacteria)
• RNA Isolation for NGS $50.00 per sample

Quality Control Service:

• Sample and Library QC

Data Storage Service:

• Data Storage (per additional 3 months)

Product and services for research use only

100% Guaranteed CRISPR Knockout Cell Line Generation Service

abm offers the first ever 100% Guaranteed CRISPR Knockout Cell Line Generation Service to our clients who want to have full confidence in their knockout models. We understand how obtaining successful knockout models can have a significant impact on your research.

Our Promise:

• Delivery or no charge
• 100% guaranteed full-allelic knockout at the protein level
• 2 cryovials provided per clone

• 90% cell viability

Our 100% Guaranteed Service is risk-free - you pay nothing if we do not deliver.

Each phase of the service comes with milestone-based reports to keep you fully updated on the status of your project. abm’s 3 phase KO cell line generation process:

**Strategy & Design**

• Consult our specialists on your project: choose from our list of cells* or use your own cell line model
• Selection of target site and prediction of off-target sites
CRISPR/Cas9 Genome Editing
• sgRNA vector design and construction
• Gene transfer efficiency optimization
• Delivery of CRISPR/Cas9 machinery into host cells
Selection, Validation, and Delivery
• Clonal screening and selection
• Validation of knockout by Sanger sequencing and Western blot
• Expansion and cryopreservation of selected clones
• Microbial/sterility testing

Please inquire for estimated lead time.

abm's Hematoxylin and Eosin (H&E) staining allows crystal clear visualization of tissue morphology by staining the nucleus and cytoplasm separately. abm also offers special histology stains which are used to stain specific cell structures such as carbohydrates, amyloid, lipids, connective tissue and pigments and minerals.

Key Features:

• Special stains available: Acid Fast Bacilli, Alcian Blue, Congo Red, Gram, Nissl, Oil Red O, Periodic Acid-Schiff, Toluidine Blue.
• Customization to fit your needs.
• Full range of add-on services including tissue or cell pellet processing, embedding, sectioning, and IHC staining for paraffin samples.
• 1-2 weeks turn-around-time and competitive pricing

Deliverables:

• Cover-slipped stained slide

Add-on Services:

• Processing of Formalin-Fixed wet tissue suitable for paraffin embedding: $5/tissue
• Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining: $4/tissue
• Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining: $7/block
• Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining: $3/slide
• Multiple sections cut in sequence by the Microtome from the same tissue block: Inquire
• Multiple sections from the same tissue block cut at set distances from one another by the Microtome: Inquire
• Digital scans of stained slides at 10X, 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services: $6/scan
• Image analysis of stained slides using professional software: $25/section
• Pathological evaluation of stained slides by trained professionals: Inquire

This service is for research use only.

abm's Hematoxylin and Eosin (H&E) staining allows crystal clear visualization of tissue morphology by staining the nucleus and cytoplasm separately. abm also offers special histology stains which are used to stain specific cell structures such as carbohydrates, amyloid, lipids, connective tissue and pigments and minerals.

Key Features:

• Special stains available: Acid Fast Bacilli, Alcian Blue, Congo Red, Gram, Nissl, Oil Red O, Periodic Acid-Schiff, Toluidine Blue.
• Customization to fit your needs.
• Full range of add-on services including tissue or cell pellet processing, embedding, sectioning, and IHC staining for paraffin samples.
• 1-2 weeks turn-around-time and competitive pricing

Deliverables:

• Cover-slipped stained slide

Add-on Services:

• Processing of Formalin-Fixed wet tissue suitable for paraffin embedding: $5/tissue
• Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining: $4/tissue
• Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining: $7/block
• Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining: $3/slide
• Multiple sections cut in sequence by the Microtome from the same tissue block: Inquire
• Multiple sections from the same tissue block cut at set distances from one another by the Microtome: Inquire
• Digital scans of stained slides at 10X, 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services: $6/scan
• Image analysis of stained slides using professional software: $25/section
• Pathological evaluation of stained slides by trained professionals: Inquire

This service is for research use only.

Cell Lines:
Immortalized Mouse Distal Caput Epididymal Epithelial Cell Line (DC2)
NFkB-Luciferase Stable RAW264.7 Cell Line
Alpha1D-adrenergic receptor Stable HEK293 Cell Line
Ba/F3 Stably Expressing Non-Transforming Kinase EPHA4 Cell Line
Ba/F3 Stably Expressing Non-Transforming Kinase ERBB2 Cell Line
Ba/F3 Stably Expressing Transforming Kinase ABL Cell Line
Ba/F3 Stably Expressing Transforming Kinase ABL2 Cell Line
Ba/F3 Stably Expressing Transforming Kinase ALK Cell Line
Ba/F3 Stably Expressing Transforming Kinase AXL Cell Line
Ba/F3 Stably Expressing Transforming Kinase BLK Cell Line
Ba/F3 Stably Expressing Transforming Kinase EPHA1 Cells
Ba/F3 Stably Expressing Transforming Kinase EPHA5 Cell Line
Ba/F3 Stably Expressing Transforming Kinase EPHA7 Cell Line
Ba/F3 Stably Expressing Transforming Kinase EPHA8 Cell Line
Ba/F3 Stably Expressing Transforming Kinase FGFR2 Cell Line
Ba/F3 Stably Expressing Transforming Kinase FGFR3 Cell Line
Ba/F3 Stably Expressing Transforming Kinase FGR Cell Line
Ba/F3 Stably Expressing Transforming Kinase FLT3 Cell Line
Ba/F3 Stably Expressing Transforming Kinase FLT4 Cell Line
Ba/F3 Stably Expressing Transforming Kinase FYN Cell Line
Ba/F3 Stably Expressing Transforming Kinase HCK Cell Line
Ba/F3 Stably Expressing Transforming Kinase IGF1R Cell Line
Ba/F3 Stably Expressing Transforming Kinase INSR Cell Line
Ba/F3 Stably Expressing Transforming Kinase JAK1 Cell Line
Ba/F3 Stably Expressing Transforming Kinase JAK2 Cell Line
Ba/F3 Stably Expressing Transforming Kinase JAK3 Cell Line
Ba/F3 Stably Expressing Transforming Kinase KIT Cell Line
Ba/F3 Stably Expressing Transforming Kinase LCK Cell Line
Ba/F3 Stably Expressing Transforming Kinase LTK Cell Line
Ba/F3 Stably Expressing Transforming Kinase MERTK Cell Line
Ba/F3 Stably Expressing Transforming Kinase MET Cell Line
Ba/F3 Stably Expressing Transforming Kinase MST1R Cell Line
Ba/F3 Stably Expressing Transforming Kinase NTRK1 Cell Line
Ba/F3 Stably Expressing Transforming Kinase NTRK2 Cell Line
Ba/F3 Stably Expressing Transforming Kinase NTRK3 Cell Line
Ba/F3 Stably Expressing Transforming Kinase PDGFRA Cell Line
Ba/F3 Stably Expressing Transforming Kinase PDGFRB Cell Line
Ba/F3 Stably Expressing Transforming Kinase RET Cell Line
Ba/F3 Stably Expressing Transforming Kinase ROS1 Cell Line
Ba/F3 Stably Expressing Transforming Kinase SRC Cell Line
Ba/F3 Stably Expressing Transforming Kinase SYK Cell Line
Ba/F3 Stably Expressing Transforming Kinase TNK1b Cell Line
Ba/F3 Stably Expressing Transforming Kinase TYRO3 Cell Line
Ba/F3 Stably Expressing Transforming Kinase YES1 Cell Line
c-Kit N705A/N938A Stable Ba/F3 Cell Line
c-Kit Y568F/Y570F Stable Ba/F3 Cell Line
c-Kit Y703F/Y936F Stable Ba/F3 Cell Line
c-Kit/D816F Stable Ba/F3 Cell Line
c-Kit/D816H Stable Ba/F3 Cell Line
c-Kit/D816V Stable Ba/F3 Cell Line
c-Kit/D816YStable Ba/F3 Cell Line
c-Kit/K642E Stable Ba/F3 Cell Line
c-Kit/N705AStable Ba/F3 Cell Line
c-Kit/N938A Stable Ba/F3 Cell Line
c-Kit/V560D Stable Ba/F3 Cell Line
c-Kit/WT Stable Ba/F3 (with GNNK- isoform) Cell Line
c-Kit/WT Stable Ba/F3 (with GNNK+ isoform) Cell Line
c-Kit/Y568F Stable Ba/F3 Cell Line
c-Kit/Y570F Stable Ba/F3 Cell Line
c-Kit/Y703F Stable Ba/F3 Cell Line
c-Kit/Y721F Stable Ba/F3 Cell Line
c-Kit/Y823F Stable Ba/F3 Cell Line
c-Kit/Y936F Stable Ba/F3 Cell Line
Conditionally Immortalized Mouse Colonic Epithelial Cells
Conditionally Immortalized Mouse Liver Cells
Conditionally Immortalized Mouse Melanocytes (iMC23)
Conditionally Immortalized Mouse Pancreas Cells
Conditionally Immortalized Mouse Small Intestinal Epithelial Cells
Conditionally Immortalized Mouse Stomach Epithelial Cells
Dual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line
Dual Notch1(Variant)-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1G4esn) Cell Line
GRPR-Luciferase stable PC3 cell line
Human Bone Marrow Mesenchymal Stem Cells – SV40
Human Carotid Artery Smooth Muscle Cells -hTERT
Human Carotid Artery Smooth Muscle Cells -SV40
Human Cerebral Microvascular Endothelial Cells-SV40
Human Normal Brachiocephalic Artery Endothelial Cells - SV40
Human Normal Cardiac Microvascular Endothelial Cells - SV40
Human Normal Carotid Artery Endothelial Cells - SV40
Human Normal Marrow Stromal Cells -SV40
Human Normal Marrow Stromal Cells -SV40-YFP
Immortalized (Conditionally) Mouse Colonic Epithelial Cells (YAMC)
Immortalized (Conditionally) Mouse Hepatocytes (ImHep)
Immortalized (Conditionally) Mouse Lung Clara Cells (C22)
Immortalized (Conditionally) Mouse Pancreas Epithelial Cells (IMPE)
Immortalized (Conditionally) Mouse Small Intestinal Epithelial Cells (MSIE)
Immortalized (Conditionally) Mouse Smooth Muscle/Myometrial-Like Cells (SMU1-10)
Immortalized (Conditionally) Mouse Stomach Epithelial Cells (ImSt)
Immortalized (Conditionally) Rat Decidual Cells (GG-AD)
Immortalized (Conditionally) Rat Luteal Cells (GG-CL)
Immortalized Baby Mouse Kidney Epithelial Cells (iBMK)
Immortalized Chicken Skeletal Muscle Cells - SV40
Immortalized Fetal Bovine Kidney Cells - SV40
Immortalized Fetal Bovine Lung Cells - SV40
Immortalized Hamster Hepatocytes -SV40
Immortalized Hamster Hepatocytes-SV40 T+t
Immortalized Human Adipose-derived Stromal Cells - Bmi-1/hTERT
Immortalized Human Adipose-derived Stromal Cells- Bmi-1/hTERT
Immortalized Human Adrenocortical Cells -hTERT
Immortalized Human Adrenocortical Cells- hTERT
Immortalized Human Adult Bone Marrow-derived Stromal Cells- SV40
Immortalized Human Alveolar Cells - Type 1
Immortalized Human Alveolar type1 Cells -SV40
Immortalized Human Alveolar type1 Cells-hTERT
Immortalized Human Amniotic Epithelial Cells - SV40
Immortalized Human Aortic Endothelial Cells- SV40
Immortalized Human Aortic Endothelial-SV40
Immortalized Human Aortic Smooth Muscle Cells - SV40
Immortalized Human Astrocyte Cells -hTERT/SV60
Immortalized human astrocytes- SV40
Immortalized Human Astrocytes, fetal - hTERT
Immortalized Human Astrocytes, fetal - SV40
Immortalized Human Benign Prostate Epithelial Cell - SV40
Immortalized Human Benign Prostate Epithelial Cell-SV40
Immortalized Human Benign Skin Cells - SV40
Immortalized Human Bladder Microvascular Endothelial Cells
Immortalized Human Bladder Microvascular Endothelial Cells
Immortalized Human Bone Marrow Basophils
Immortalized Human Bone Marrow Mesenchymal Cells - hTERT
Immortalized Human Bone Marrow Mesenchymal Cells – hTERT
Immortalized Human Bone Marrow Mesenchymal Stem Cells SV40
Immortalized Human Bone Marrow Mesenchymal Stromal Cells - hTERT
Immortalized Human Bone Marrow Mesenchymal Stromal Cells - hTERT (iMSC3)
Immortalized Human Bone Marrow Mononuclear Cells
Immortalized Human Bone Marrow-derived Stromal Cells - SV40 - YFP
Immortalized Human Brachiocephalic Artery Endothelial Cells - SV40
Immortalized Human Brachiocephalic Artery Smooth Muscle Cells
Immortalized Human Bronchial Endothelial Cells
Immortalized Human Bronchial Epithelial Cells - SV40
Immortalized Human Bronchial Epithelial Cells-hTERT
Immortalized Human Bronchial Epithelial Cells-hTERT/BMI
Immortalized Human Bronchial Epithelial Cells-hTERT+BMI
Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4
Immortalized Human Bronchial Epithelial Cells-SV40
Immortalized Human Bronchial Smooth Muscle Cells
Immortalized Human Bronchiole Endothelial Cells
Immortalized Human Cardiac Fibroblasts
Immortalized Human Cardiac Fibroblasts
Immortalized Human Cardiac Microvascular Endothelial Cells - SV40
Immortalized Human Cardiomyocytes - SV40
Immortalized Human Cardiomyocytes-SV40
Immortalized Human Carotid Artery Endothelial Cells - SV40
Immortalized Human Carotid Artery Smooth Muscle Cells
Immortalized Human Carotid Artery Smooth Muscle Cells - hTERT
Immortalized Human Carotid Artery Smooth Muscle Cells - SV40
Immortalized Human Cerebral Microvascular Endothelial Cells - Myc
Immortalized Human Cerebral Microvascular Endothelial cells - P53
Immortalized Human Cerebral Microvascular Endothelial Cells - Ras
Immortalized Human Cerebral Microvascular Endothelial Cells - SV40
Immortalized Human Chondrocytes - hTERT
Immortalized Human Chondrocytes - Myc
Immortalized Human Chondrocytes - Ras
Immortalized Human Chondrocytes - SV40
Immortalized Human Colon Cells
Immortalized Human Colon Cells
Immortalized Human Conjuctival Epithelial Cells - SV40
Immortalized Human Conjunctiva Fibroblast - SV40
Immortalized Human Conjunctival Epithelial Cells
Immortalized Human Conjunctival Fibroblast
Immortalized Human Cord Blood Mesenchymal Stromal cells
Immortalized Human Cord Blood Mesenchymal Stromal cells with RFP
Immortalized Human Corneal Endothelial Cells
Immortalized Human Corneal Endothelial Cells (IHCE)
Immortalized Human Corneal Epithelial Cells
Immortalized Human Corneal Fibroblasts
Immortalized Human Corneal Fibroblasts (IHCF)
Immortalized Human Corneal Keratinocytes
Immortalized Human Corneal Keratinocytes (IHCK)
Immortalized Human Coronary Artery Endothelial Cells
Immortalized Human Coronary Artery Endothelial Cells
Immortalized Human Coronary Artery Smooth Muscle Cells
Immortalized Human Dendritic Cells
Immortalized Human Dermal Microvascular Endothelial Cells
Immortalized Human Dermal Papilla Cells
Immortalized Human Diploid Fibroblasts - hTERT
Immortalized Human Dopamine Neuronal Cells
Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES)
Immortalized Human Endocervical Epithelial Cells (A2EN)
Immortalized Human Endometrial Stromal Cells (HESC)
Immortalized Human Endoneurial Endothelial Cell Line
Immortalized Human Endoneurial Endothelial Cells (THEndEC)
Immortalized Human Epidermal Keratinocytes
Immortalized Human Fallopian Tube Secretory Epithelial cells
Immortalized Human Fallopian Tube Secretory Epithelial Cells (FT33-shp53-R24C)
Immortalized Human Fetal Astrocytes - hTERT
Immortalized Human Fetal Astrocytes - SV40
Immortalized Human Fetal Liver Stromal Cells
Immortalized Human Fetal Osteoblast - SV40
Immortalized Human Fetal Osteoblastic Cell Line (SV40-tsA58)
Immortalized Human Fetal Osteoblastic estrogen receptor stable Cell Line - SV40
Immortalized Human Fetal Osteoblastic estrogen receptor stable Cell Line (SV40-tsA58)
Immortalized Human Follicle Outer Root Sheath Cells
Immortalized Human Follicle Outer Root Sheath Cells
Immortalized Human Fungiform Taste Cells - SV40
Immortalized Human Fungiform Taste Cells – SV40
Immortalized Human Gingival Fibroblast -SV40
Immortalized Human Gingival Fibroblast hTERT
Immortalized Human Gingival Fibroblast- SV40
Immortalized Human Gingival Fibroblasts- hTERT
Immortalized Human Gingival Keratinocytes (Gie-No3B11)
Immortalized Human Granulosa Cell Line (HGL5)
Immortalized Human Granulosa Cells (HGL5)
Immortalized Human Hair Follicle Dermal Papilla Cells - SV40
Immortalized Human Hair Follicle Dermal Papilla Cells –SV40T/c-Myc
Immortalized Human Hair Follicle Dermal Papilla Cells –SV40T/c-Myc
Immortalized Human Hair Follicle Papilla Cells - hTERT
Immortalized Human Hair Follicle Papilla Cells -SV40
Immortalized Human Hepatic Non-Parenchymal Cells
Immortalized Human Hepatic Sinusoidal Endothelial Cells-SV40
Immortalized Human Hepatocytes - SV40
Immortalized Human Iliac Artery Endothelial Cells
Immortalized Human Internal Thoracic Artery Endothelial Cells
Immortalized Human Internal Thoracic Artery Smooth Muscle Cells
Immortalized Human Intervertebral Disc Cartilage Endplate-derived Stem Cells -SV40
Immortalized Human Intestinal Microvascular Endothelial Cells-SV40
Immortalized Human Laryngeal Posterior Commissure Cells
Immortalized Human Lens Epithelial Cells - SV40
Immortalized Human Lens Epithelial Cells- SV40
Immortalized Human Lingual Cells
Immortalized Human Lung Epithelial Cells
Immortalized Human Lung Epithelial Cells
Immortalized Human Lung Fibroblast - hTERT
Immortalized Human Mammary Epithelial Cells
Immortalized Human Mammary Epithelial Progenitor (K5+/K19+) Cells - hTERT
Immortalized Human Mammary MUC+ESA+ Epithelial Cells
Immortalized Human Mesangial Cells
Immortalized Human Mesothelial Cells - SV40
Immortalized Human Mesothelial Cells-SV40
Immortalized Human Microglia - SV40
Immortalized Human Nasal Epithelial Cells - SV40
Immortalized Human Neonatal Fibroblasts- hTERT
Immortalized Human Olfactory Ensheathing Glia Bmi1/hTERT
Immortalized Human Olfactory Ensheathing Glia Bmi1/hTERT/SV40
Immortalized Human Olfactory Ensheating Glia-Bmi1/hTERT
Immortalized Human Olfactory Ensheating Glia-Bmi1/hTERT/SV40
Immortalized Human Oral Epithelial Cells
Immortalized Human Oral Keratinocytes
Immortalized Human Oropharyngeal Cells
Immortalized Human Osteoblasts- SV40
Immortalized Human Ovarian Epithelial Cells - SV40
Immortalized Human Pancreas Cells - SV40
Immortalized Human Pancreatic Cells - Myc
Immortalized Human Pancreatic Cells - SV40
Immortalized Human Pancreatic Duct Epithelial Cells - HPV16 E6/E7
Immortalized Human Pancreatic Islet-Derived Precursor Cells - EBV
Immortalized Human Pancreatic Islet-Derived Precursor Cells - hTERT
Immortalized Human Pancreatic Islet-Derived Precursor Cells - Myc
Immortalized Human Pancreatic Islet-Derived Precursor Cells - Ras
Immortalized Human Pancreatic Islet-Derived Precursor Cells - SV40
Immortalized Human Pancreatic Mesenchymal Stem Cells - hTERT
Immortalized Human Pancreatic Mesenchymal Stem Cells - Myc
Immortalized Human Pancreatic Mesenchymal Stem Cells - Ras
Immortalized Human Pancreatic Mesenchymal Stem Cells - SV40
Immortalized Human Periodontal Ligament Fibroblasts
Immortalized Human Placental Epithelial Cells - SV40
Immortalized Human Primary Urothelial Cells
Immortalized Human Prostate Epithelial Cells
Immortalized Human Prostate Smooth Muscle Cells
Immortalized Human Prostate Stromal Cells
Immortalized Human Pterygium Epithelial Cells - SV40
Immortalized Human Pterygium Fibroblast - SV40
Immortalized Human Pulmonary Artery Endothelial Cells
Immortalized Human Pulmonary Artery Smooth Muscle Cells
Immortalized Human Pulmonary Fibroblasts Cells
Immortalized Human Pulmonary Microvascular Endothelial Cells
Immortalized Human Renal Cortical Epithelial Cells
Immortalized Human Renal Cortical Epithelial Cells
Immortalized Human Renal Epithelial Cells
Immortalized Human Renal Epithelial Cells
Immortalized Human Renal Medullary Epithelial Cells
Immortalized Human Renal Medullary Epithelial Cells
Immortalized Human Renal Proximal Tubule Cells
Immortalized Human Renal Proximal Tubule Cells
Immortalized Human Retinal Pigment Epithelial Cells
Immortalized Human Retinal Pigment Epithelial Cells
Immortalized Human Sertoli Cells- SV40
Immortalized Human Sertoli Cells- SV40
Immortalized Human Skeletal Muscle Cells
Immortalized Human Skeletal Muscle Cells
Immortalized Human Skeletal Muscle Myoblasts
Immortalized Human Skeletal Muscle Myoblasts
Immortalized Human Skin Fibroblast - Myc
Immortalized Human Skin Fibroblast - SV40
Immortalized Human Small Airway Epithelial Cells - SV40
Immortalized Human Subclavian Artery Endothelial Cells
Immortalized Human Subclavian Artery Smooth Muscle Cells
Immortalized Human Synovial Membrane Chondroblasts Cells
Immortalized Human Synovial Membrane Fibroblasts -SV40
Immortalized Human Testicular Cells
Immortalized Human Total Liver Cell Population
Immortalized Human Tracheal Epithelial Cells
Immortalized Human Tracheal Epithelial Cells
Immortalized Human Tracheal Smooth Muscle Cells
Immortalized Human Umbilical Artery Endothelial Cells
Immortalized Human Umbilical Artery Smooth Muscle Cells
Immortalized Human Umbilical Vein Endothelial Cells (SV40)
Immortalized Human Umbilical Vein Smooth Muscle Cells
Immortalized Human Urinary Bladder Smooth Muscle Cells
Immortalized Human Urinary Bladder Smooth Muscle Cells
Immortalized Human Urothelial Cells
Immortalized Human Uterine Fibroblasts
Immortalized Human Uterine Microvascular Endothelial Cells
Immortalized Human Vascular Endothelial Cells (HPV 16 E6/E7)
Immortalized Monkey Hepatocytes -SV40
Immortalized Monkey Hepatocytes-SV40
Immortalized Monkey Hepatocytes-SV40 T+t
Immortalized mouse hepatocytes - SV40
Immortalized Mouse Achilles Tendon Cells (iMAT)
Immortalized Mouse Articular Chondrocytes 
Immortalized Mouse Articular Chondrocytes (iMAC)
Immortalized Mouse Astrocytes - SV40T (IMA2.1)
Immortalized Mouse Astrocytes- SV40 T
Immortalized Mouse AT1A (-/-), AT1B (-/-), Tag (+/+) Proximal Cells
Immortalized Mouse AT1A (-/-), Tag (+/-) Proximal Tubule Cells
Immortalized Mouse AT1B (-/-), Tag (+/-) Proximal Tubule Cells
Immortalized Mouse AT2 (-/-) Proximal Tubule Cells
Immortalized Mouse Brain Endothelial Cells - hTERT
Immortalized Mouse Brain Endothelial Cells - Myc
Immortalized Mouse Brain Endothelial Cells - Ras
Immortalized Mouse Brain Endothelial Cells - SV40
Immortalized Mouse Brain Microvascular Endothelial Cells (cerebellar cortex)
Immortalized Mouse Brain Microvascular Endothelial Cells (cerebral cortex)
Immortalized Mouse Cardiomyogenic Progenitor Cells   
Immortalized Mouse Cardiomyogenic Progenitor Cells (iCP)
Immortalized Mouse Cementoblasts
Immortalized Mouse Cementoblasts (CM/PDL)
Immortalized Mouse Cerebellar Capillary Endothelial Cells (cerebEND)
Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND)
Immortalized Mouse Cortical Collecting Duct Cell Line (mCCD(cl1))
Immortalized Mouse Dendritic Cells
Immortalized Mouse Dental Apical Papilla Progenitor Cells
Immortalized Mouse Dental Apical Papilla Progenitor Cells (iSCAP)
Immortalized Mouse Distal Caput Epididymal Epithelial Cell Line (DC2)
Immortalized Mouse Embryonic Hepatic Progenitor Cells
Immortalized Mouse Embryonic Hepatic Progenitor Cells (iHP)
Immortalized Mouse Epidermal Cells (COCA)
Immortalized Mouse Hepatic Sinusoidal Endothelial Cells - SV40
Immortalized mouse hepatic sinusoidal endothelial cells- SV40
Immortalized Mouse Hepatocytes - SV40
Immortalized Mouse Intermediately Differentiated Melanoblasts (iMC37)
Immortalized Mouse Intestinal Myofibroblasts
Immortalized Mouse Kidney Epithelial Cells (iBMK) 
Immortalized Mouse Late-Stage Melanocytes (iMC65)
Immortalized Mouse Lung Clara Cell Line
Immortalized Mouse Lung Clara Cell Line- SV40 (mTCC)
Immortalized Mouse Lung Clara Cells- SV40 (mTCC)
Immortalized Mouse Lung Epithelial Cells (MLE-15)
Immortalized Mouse Mammary Epithelial Cells (iMMEC)
Immortalized Mouse Melanocytes  
Immortalized Mouse Mesangial Cells - SV40
Immortalized Mouse Microglia
Immortalized Mouse Microglia (SIM-A9)
Immortalized Mouse Ovarian Surface Epithelial Cells (iMOSEC)
Immortalized Mouse Prostate Epithelial Cells (iMPEC)
Immortalized Mouse Prostate Epithelial Cells (iMPEC)   
Immortalized Mouse Proximal Caput Epididymal Epithelial Cell Line (PC1)
Immortalized Mouse Proximal Tubule Cells
Immortalized Mouse Retinal Pigmented Epithelial Cells - HPV16 E6/E7
Immortalized Mouse Retinal Pigmented Epithelial Cells- HPV 16 E6/E7
Immortalized Mouse Schwann Cells (IMS32)
Immortalized Ovarian Surface Epithelial Cells (iMOSEC)   
Immortalized Pancreatic duct epithelial cells - HPV E6/E7
Immortalized PARP1(1/1) Murine Endothelial (HYKO6)
Immortalized Porcine Aortic Endothelial Cells (AOC)
Immortalized Pulmonary Artery Endothelial Cells
Immortalized Rat Arachnoid Cells - SV40/hTERT
Immortalized Rat Arachonoid Cells - SV40-hTERT
Immortalized Rat Cardiomyocytes
Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A)
Immortalized Rat Granulosa cells stably expressing FSH Receptor
Immortalized Rat Granulosa cells stably expressing LH Receptor
Immortalized Rat Hepatocytes - SV40
Immortalized Rat Kupffer Cells - SV40
Immortalized Rat Kupffer Cells- SV40
Immortalized Rat Lung Epithelial Cell Line
Immortalized Rat Metanephric Mesenchymal Precursor Cells (KMM)
Immortalized Rat Microglia-SV40
Immortalized Rat Odontoblast Cells
Immortalized Rat Olfactory Ensheathing Glia SV40
Immortalized Rat Olfactory Ensheating Glia- SV40
Immortalized Rat Periodontal Ligament Cells
Immortalized Rat Pre-Type II Alveolar Epithelial Cell Line
Immortalized Rat Pre-Type II Alveolar Epithelial Cells, Fetal
Immortalized Rat Retinal Muller Cell Line
Immortalized Rat Retinal Müller Cells (rMC-1)
Immortalized Rat Retinal Precursor Cell Line
Immortalized Rat Retinal Precursor Cells (R28)
Immortalized Rat Schwann Cells
Immortalized Rat Schwann Cells (IFRS1)
Immortalized Rat Steroidogenic Granulosa Cells stably expressing FSH Receptor (GFSHR-17)
Immortalized Rat Steroidogenic Granulosa Cells stably expressing LH/CG Receptor (GLHR-15)
Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2)
Immortalized Rat Sympathoadrenal Progenitor Cells (MAH-A3)
Immortalized Synovial Membrane Fibroblasts -SV40
ImmortalizedHuman Hair Follicle Dermal Papilla Cells - hTERT
Isogenic  Atg5 Knockdown Immortalized Mouse Kidney Epithelial Cells
Isogenic  Atg7 Knockdown Immortalized Mouse Kidney Epithelial Cells 
Isogenic  Bak Knockdown Immortalized Mouse Kidney Epithelial Cells
Isogenic  Bak/Bax Double Knockdown Immortalized Mouse Kidney Epithelial Cells
Isogenic  Bax Knockdown Immortalized Mouse Kidney Epithelial Cells
K5+/K19+ hTERT-Immortalized Human Mammory Stem/Progenitor Cells
KCC2 over expressing HEK293 Cells
mmortalized Mouse CD4+ CD8+ T Cells (MOHITO)
Nav2.1/β1/Contactin Stable Chinese Hamster Lung Cell Line
PGC-1α/ERRα Stable 293T Cell Line
RyR1 HEK293 Stable Cell Line (tech ID 6-15037)
TLR2 Stable HEK293 Cell Line
TLR3 KO Immortalized Mouse Dendritic Cells
TLR9 KO Immortalized Mouse Dendritic Cells
Type I IFN KO Immortalized Mouse Dendritic Cells

abm has over 10 years of experience immortalizing nearly every human and mouse cell lines using different immortalizing reagents. If you have a primary cell line that you would like to immortalize, our risk-free immortalization services are the quickest and most reliable way for your project.

Service Description:
Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimen and will provide a consistent cellular material, capable of extended proliferation and possess similar or identical genotype and phenotype as the parental tissue, throughout the duration of your research experiment.

Key Service Features:

• Risk-free! Request no payment until immortalization is complete*
• Viral transduction on primary cells submitted
• Cell immortalization
• Drug selection on successfully transduced cells
• Clone screening and expansion confirmation of immortalization via qRT-PCR of Transgene
• Cryopreservation and delivery of cells

*A non-refundable 15% deposit is required to initate the project

Deliverables:

• Up to two clones, 2 vials/clone
• Any additional add-ons where applicable
  Service report includes:
• Morphology Assessment
• qPCR Analysis of transgene expression
• Test results for presence or absence of microbial contaminants including bacterial, fungal, and mycoplasma testing

Add-on Service:

• Additional clones: $250.00USD
• Additional vials of delivered clones: $80.00USD
• Cell line authentication service: $150.00USD

abm offers a variety of molecular cloning services that suit your experiment needs. Our offerings consist of the services below:

Economy Gene Synthesis & Subcloning - $250.00

• For non-complicated sequences
• Synthesize up to 950bp
• Complimentary subcloning into pUC19
• Sequence verification to confirm full length sequence is generated
• Sequence must be approved for economy synthesis by abm

Gene synthesis - $0.18/ bp

• For any sequence up to 4kb in size
• Genes are fully sequenced
• Must be ordered with Subcloning Service I (C096)

• minimum charge of $99.00

Subcloning Service I - $125.00

• Subclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are compatible with the expression vector of choice.
• End sequencing of the insert is included with this service to confirm proper insertion*

• For full sequencing, please refer to our DNA (Sanger) Sequencing service (C199) below

Subcloning Service II - $225.00

• Subclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are not compatible with the expression vector of choice.
• Full length DNA sequencing is included for sequence verification
  *Genes over 3kb are subject to an additional charge of $100/kb

Site-Directed Mutagenesis - $150.00*

• Mutate up to 25bp (consecutive) within a <1kb fragment in any existing abm product or a provided vector.
• Full length sequencing to confirm presence of mutation

• Fragments over 1kb are subject to an additional charge of $100/kb

We also have additional services to supplement the ones above:

Promoter Change Service - $350.00

• Change the promoter in one of abm’s vectors or a vector of your choice to any of the following: CMV, EF1a, PGK, UbC

Tag Addition/Removal Service (≤30bp) - $250.00

• Addition/Removal of an N-terminal or C-terminal tag smaller than 30bp

Tag Addition/Removal Service (>30bp) - $350.00

• Addition/Removal of an N-terminal or C-terminal tag larger than 30bp

• Tags over 1kb are subject to an additional charge of $100/kb

Reporter Addition/Removal Service - $350.00

• Addition, removal or switching of a GFP or RFP reporter in one of abm’s vectors or a vector of your choice

Antibiotic Switching Service - $350.00

• Switching of a Puromycin resistance gene to Neomycin resistance in one of abm’s vectors or a vector of your choice.

DNA (Sanger) Sequencing - $5.50/reaction

• Verify the sequence and position of your gene of interest or sequence your PCR products

DNA Sequencing with Custom Primer Synthesis - $11.50/reaction

• Verify the sequence and position of your gene of interest or sequence your PCR products.

Note: For research use only

Immunohistochemistry (IHC) exploits antigen-antibody reactions for the detection of targets-of-interest in tissue samples. abm's IHC service is able to localize and quantify any antigen of your choice in tissues. The high sensitivity and specificity makes IHC perfect for pathological diagnostics and drug R&D.

Key service Features:

• IHC staining using over 200 validated high-specificity antibodies for a variety of pathological markers.
• High throughput Leica Bond Autostainer provides consistent and reliable staining results.
• Full range of add-on histology services including tissue or cell pellet processing, embedding, sectioning and routine or special staining for paraffin samples.
• 1 week turn-around-time.

Deliverables:

• Cover-slipped stained IHC slide

Basic Service: $25/slide

• IHC staining of existing sectioned FFPE slides with customer provided antibody

Complete Service: $30/slide

• IHC staining of existing sectioned FFPE slides with ABM's antibody

Dual Staining Service: Inquire

• IHC staining of existing sectioned FFPE slides for two markers-of-interest with customer provided antibody
• Simultaneous detection of mouse antibody and rabbit antibody

Service Requirements

• Section the tissue at 4μm
• Provide 20μg of primary antibody with optimal dilution and a positive control slide (Basic and Dual staining service)

Add-on Services:

• Processing of Formalin-Fixed wet tissue suitable for paraffin embedding: $5/tissue
• Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining: $4/tissue
• Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining: $7/block
• Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining: $3/slide
• Multiple sections cut in sequence by the Microtome from the same tissue block: Inquire
• Multiple sections from the same tissue block cut at set distances from one another by the Microtome: Inquire
• Digital scans of stained slides at 10X, 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services: $6/scan
• Image analysis of stained slides using professional software: $25/section
• Pathological evaluation of stained slides by trained professionals: Inquire

This service is for research use only.

IHC Optimization Services - Starting at $350 USD per sample

Optimization of the pretreatment and working concentration for IHC antibody is crucial to obtain the best staining results, which is to have the minimum amount of background with the strongest staining intensity. abm's IHC Optimization Service will find the best IHC staining conditions for your antibody.

Key Features:

• Tests 3 different pretreatment conditions at 4 different dilutions
• Provides all 12 images
• 2-3 weeks turn-around-time

Service Requirements:

• Customer to provide 30μg of primary antibody and one positive control tissue block

This service is for research use only

For any gene sequence up to 4kb in size, we offer custom service at the unbeatable price of $0.18/bp, as well as subcloning your gene of interest into an expression system of your choice at the starting price of $125.00. Also included in this service is full sequencing of the gene to confirm insertion.

• cost effective
• must be ordered with Subcloning Service I (C096)
• clonable into any one of our in house vectors (at addition cost, please contact us for a quote as prices vary depending on gene size and vector choice)
• genes are fully sequenced
• *minimum charge of $99.00

Note: For research use only

abm's Metagenomics Sequencing employs an efficient and cost effective way to sequence DNA from environmental and microbiome samples. Whether your target of interest is 16S, 18S, ITS, or complete genomes, let abm's sequencing and bioinformatics expertise help you make your next microbial discoveries.

Key features:

• Guarantee minimum of 300,000 reads per sample
• Multiplex and sequence up to 104 samples in a single run
• Economical pricing starting at only $99 per sample
• Fast turn-around-time of 3-4 weeks
• 3 months of free data storage

Applications:

• 16S / 18S/ ITS metagenomics

Deliverables:

• Sequencing results (FASTQ)
• Bioinformatics analysis
• Contig assembly
• OTU clustering and classification
• Alpha/Beta diversity index analysis
• Full QC and bioinformatics report

Metagenomics Sequencing Services:

• Metagenomics Sequencing Service (1-23 samples): $199 per sample
• Metagenomics Sequencing Service (24-48 samples): $99 per sample
• Metagenomics Sequencing Service (49-98 samples): $45 per sample

Bioinformatics Services: $30.00 per sample
Includes:

• Contig assembly
• OTU clustering and classification
• Alpha/Beta diversity index analysis

Additional Sample Preparation Services

• DNA Isolation for NGS: $50.00 per sample

Data Storage Service:

• Addtional 3 months: $150 per sample

Product and services intended for research use only

abm’s Illumina certified sequencing service delivers full plasmid sequencing and assembly starting with only 1ng of plasmid DNA and a turn-around-time of 2-4 weeks. Having questions about your plasmid backbone? Let us help you!

Key features:

• Start with 1ng of plasmid DNA
• de novo assembly of plasmid sequence included.
• Fast turn-around time (typically 2-4 weeks)

Deliverables:

1. Sequencing results (FASTQ)
2. Assembled plasmid sequence
3. Plasmid annotation
4. QC and full bioinformatics report

Plasmid Sequencing Services

• Full length plasmid sequencing (100Mb, 2x75bp, PE): $165.00 per sample
• End-sequence validation (Sanger sequencing): $30.00 per sample

Bioinformatics Services

• Basic Bioinformatics Service (included):
• de novo assembly
• Plasmid annotation

Data Storage Service

• Data Storage (per additional 3 months): $150 per sample

Product and services intended for research use only

abm's Amplicon Sequencing is an econonical way to sequence a DNA fragment up to 600bp and analyze for genetic variations between large number of samples or within a single sample. Applicable for detecting rare variants or validating CRISPR mutations.

Key features:

• Ultra deep sequencing of PCR amplicons up to 600bp
• Multiplex and sequence up to 104 samples in a single run
• Economical pricing starting at only $45 per sample
• Fast turn-around-time of 2-4 weeks
• 3 months of free data storage

Applications:

• Detection of rare variants in known genes
• Validation of CRISPR induced mutations
• Metagenomics

Deliverables:

• Sequencing results (FASTQ)
• Detected variations (if applicable)
• Full QC and bioinformatics report

Amplicon Sequencing Services

• Amplicon-Seq Service (1-23 samples): $199 per sample
• Amplicon-Seq Service (24-47 samples): $99 per sample
• Amplicon-Seq Service (48-104 samples): $45 per sample

Bioinformatics Services:

• Alignment and variation detection: $20.00 per sample

Additional Sample Preparation Services

• DNA Isolation for NGS: $50.00 per sample

Data Storage Service:

• Addtional 3 months: $150 per sample

Product and services intended for research use only

abm's Hematoxylin and Eosin (H&E) staining allows crystal clear visualization of tissue morphology by staining the nucleus and cytoplasm separately. abm also offers special histology stains which are used to stain specific cell structures such as carbohydrates, amyloid, lipids, connective tissue and pigments and minerals.

Key Features:

• Special stains available: Acid Fast Bacilli, Alcian Blue, Congo Red, Gram, Nissl, Oil Red O, Periodic Acid-Schiff, Toluidine Blue.
• Customization to fit your needs.
• Full range of add-on services including tissue or cell pellet processing, embedding, sectioning, and IHC staining for paraffin samples.
• 1-2 weeks turn-around-time and competitive pricing

Deliverables:

• Cover-slipped stained slide

Add-on Services:

• Processing of Formalin-Fixed wet tissue suitable for paraffin embedding: $5/tissue
• Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining: $4/tissue
• Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining: $7/block
• Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining: $3/slide
• Multiple sections cut in sequence by the Microtome from the same tissue block: Inquire
• Multiple sections from the same tissue block cut at set distances from one another by the Microtome: Inquire
• Digital scans of stained slides at 10X, 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services: $6/scan
• Image analysis of stained slides using professional software: $25/section
• Pathological evaluation of stained slides by trained professionals: Inquire

This service is for research use only.

abm’s Illumina certified WGS sequencing service delivers high quality sequencing data with only 1ng of starting material and a turn-around-time of 2-4 weeks. Bacterial whole genome sequencing also available.

Feature service: Human WGS 30X for only $1850.00 USD

Key features:

• Low DNA input requirement (1ng for prokaryote and 50ng for eukaryote)
• Flexible sequencing scale for resequencing studies or de novo genome assembly
• Bioinformatics services available from read alignment to de novo genome assembly
• Fast turn-around time (typically 2-4 weeks)

Deliverables:

1. Sequencing results (FASTQ)
2. Assembled contig and scaffold sequences (if applicable)
3. Detected genomic variations (if applicable)
4. QC and full bioinformatics report

Whole Genome Sequencing Services

• Whole Genome Sequencing (1Gb, 2x75bp, PE)
• Whole Genome Sequencing (5Gb, 2x75bp, PE)
• Whole Genome Sequencing (10Gb, 2x75bp, PE)
• Whole Genome Sequencing (90Gb, 2x75bp, PE)

Bioinformatics Services:

• Basic Bioinformatics Service
• Read alignment
• De-duplication, base quality recalibration, and local reassembly
• SNP and indel calling
• Unified genome variation detection (Included only with de novo genome assembly)
• de novo Genome Assembly:
• de novo assembly (< 10Mb Genome)
• de novo assembly (> 10Mb Genome)
• Genome annotation
• Gene prediction
• Repetitive element
• Functional annotation (GO, KEGG, PFAM, CAZY)

Additional Sample Preparation Services:

• DNA Isolation for NGS

Data Storage Service:

• Data Storage (per additional 3 months)

Product and services intended for research use only

Immunohistochemistry (IHC) exploits antigen-antibody reactions for the detection of targets-of-interest in tissue samples. ABM's IHC service is able to localize and quantify any antigen of your choice in tissues. The high sensitivity and specificity makes IHC perfect for pathological diagnostics and drug R&D.

Key service Features:

• IHC staining using over 200 validated high-specificity antibodies for a variety of pathological markers.
• High throughput Leica Bond Autostainer provides consistent and reliable staining results.
• Full range of add-on histology services including tissue or cell pellet processing, embedding, sectioning and routine or special staining for paraffin samples.
• 1 week turn-around-time.

Deliverables:

• Cover-slipped stained IHC slide

Basic Service: $25/slide

• IHC staining of existing sectioned FFPE slides with customer provided antibody

Complete Service: $30/slide

• IHC staining of existing sectioned FFPE slides with ABM's antibody

Dual Staining Service: Inquire

• IHC staining of existing sectioned FFPE slides for two markers-of-interest with customer provided antibody
• Simultaneous detection of mouse antibody and rabbit antibody

Service Requirements

• Section the tissue at 4um
• Provide 20ug of primary antibody with optimal dilution and a positive control slide (Basic and Dual staining service)

Add-on Services:

• Processing of Formalin-Fixed wet tissue suitable for paraffin embedding: $5/tissue
• Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining: $4/tissue
• Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining: $7/block
• Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining: $3/slide
• Multiple sections cut in sequence by the Microtome from the same tissue block: Inquire
• Multiple sections from the same tissue block cut at set distances from one another by the Microtome: Inquire
• Digital scans of stained slides at 10X, 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services: $6/scan
• Image analysis of stained slides using professional software: $25/section
• Pathological evaluation of stained slides by trained professionals: Inquire

This service is for research use only.

abm's has the largest collection of sgRNA vector to knock-out any gene. Our sgRNA is lentivirus compatible and AAV compatible. We guarantee knock-out using our 3-in-1 target set.

Key Features:

• Guarantee knock-out targeting any gene
• Cloned in lentivirus compatible or AAV compatible vectors
• Available as single sgRNA vector or All-in-One vector (includes Cas9 gene)
• Lentivirus or AAV packaging also available.

Custom sgRNA Lentivirus production

• Custom sgRNA Lentivirus (1 target): $85.00
• Custom sgRNA Lentivirus set (3 target-set): $235.00
• Custom sgRNA All-in-One Lentivirus (1 target): $85.00
• Custom sgRNA All-in-One Lentivirus set (3 target-set): $245.00

Custom sgRNA AAV plasmid cloning service

• spCas9 sgRNA AAV vector (1 target): $135.00
• spCas9 sgRNA AAV vector (3-target set): $335.00
• saCas9 sgRNA AAV vector (1 target): $135.00
• saCas9 sgRNA AAV vector (3-target set): $335.00
• saCas9 All-in-One AAV vector (1 target): $185.00
• saCas9 All-in-One AAV vector (3-target set): $385.00

Note: We guarantee gene knock-out using our 3 target-set.

abm offers custom Baculovirus production that can accommodate large gene inserts and allow lifelong infection to achieve high level gene expression in host cells with post translational modifications

Key Features:

• Delivery of large transgenes (up to >100Kb)
• Generate large amount of recombinant protein (1-500mg/L)
• Post-translational modifications possible

Custom Baculovirus Production
Custom Baculovirus Production (10^6 IU/ml): $750.00 / 10ml
Custom Baculovirus Production (10^7 IU/ml): $1,550.00 / 2 x 200ul

Baculovirus Controls
Baculovirus GFP Control Virus (10^6 IU/ml): $695.00 / 10ml
Baculovirus GFP Control Virus (10^7 IU/ml): $1495.00 / 2 x 200ul

Custom Baculovirus Plasmid Cloning
Custom Baculovirus Plasmid Cloning with Compatible Insert (up to 3kb): $550.00
Custom Baculovirus Plasmid Cloning without Compatible Insert (up to 3kb): $650.00
Additional Charge for Extra 1kb: $100.00

Insert synthesis Service
Insert synthesis up to 8Kb: $0.18/bp
minimum charge $99.00

Note: For Research Use Only

Quantity: 1.0 ml
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system offers cutting edge RNA-Guided endonuclease technology for highly specific and customizable genome editing. With the use of a target sequence specific guide RNA, a highly specific and programmable nuclease and CRISPR associated nuclease (Cas9), abm offers a wide selection of Genome-Wide CRISPR/Cas9 KnockOut cell lines, including over 50,000 combinations of any human genes and all the common cell lines.

Please refer to the Resources section for a list of all the cell lines.
Vector: pLenti-U6-sgRNA-PGK-Neo
Unit: 1X 106 cells/ml
Quality Control: Surveyor assay
Price for academic institutions: $4750
Price for commercial or for-profit organizations: Inquire

If you can't find your cell line on our list, abm offers Custom-Designed Stable Cell Knockout Cell line Generation Service with abm's CRISPR System. Please contact us for a custom quotation.

abm also provides Knock-In Cell Line Generation Service and please contact us for a custom quotation.

abm’s Illumina certified WES service focuses on sequencing the coding regions to achieve high coverage suited for accurate and reliable variant discovery. Short turn-around time (2-4 weeks) and competitive pricing means solving your large scale biological questions faster.

Key features:

• Sequencing from 30X to ultra deep coverage.
• Fast turn-around time of 2-4 weeks.

Human Whole Exome Sequencing Services

• Exome Sequencing (45Mb Exome, 40X Raw data coveragel)
• Exome Sequencing (45Mb Exome, 100X Raw data coverage)
• Exome Sequencing (45Mb Exome, 135X Raw data coverage)
• Exome Sequencing (non-human species)

Bioinformatics Analysis

• Exome-Seq Analysis
• read mapping
• deduplication, local realignment, base quality recalibration
• variation calling

Other bioinformatics analyses are available. Please contact us for a quote.

Additional Sample Preparation Services

• DNA Isolation for NGS

Data Storage Service

• Data Storage (per additional 3 months)

Product and services intended for research use only

abm's Illumina certified Small RNA-seq service produces high quality strand-specific sequencing data from low sample input.

Key features:

• Starting material from 100ng of total RNA, poly-A enriched mRNA, or rRNA depleted RNA
• Strand specific single-end sequencing
• Fast turn-around time (2-3 weeks)

Small RNA-seq service includes:

• Library Preparation.
• QC (RSeQC/FastQC).
• 3 months FREE storage of raw data

Deliverables:

1. Sequencing results (FASTQ)
2. QC and full analysis report

Small RNA Sequencing:

• miRNA/Small RNA Sequencing (10 million, 1x75bp SE)

Additional Sample Preparation Services:

• rRNA Depletion Treatment (human, mouse, or rat)
• rRNA Depletion Treatment (plant or bacteria)
• RNA Isolation for NGS $50.00 per sample

Quality Control Service:

• Sample and Library QC

Data Storage Service:

• Data Storage (per additional 3 months)

Product and services for research use only

The DCM, HCM, and cardiomyopathy panels target detection of mutations in 37, 31, and 56 genes, respectively, by next-generation sequencing of all coding exons plus at least 5 bases into the 5' and 3' ends of all the introns. Additional Sanger sequencing is performed for any regions missing or with insufficient read depth coverage for reliable heterozygous variant detection.

The DCM, HCM, and cardiomyopathy panels target detection of mutations in 37, 31, and 56 genes, respectively, by next-generation sequencing of all coding exons plus at least 5 bases into the 5' and 3' ends of all the introns. Additional Sanger sequencing is performed for any regions missing or with insufficient read depth coverage for reliable heterozygous variant detection.

abm is proud to launch an express DNA sequencing service for our valued customers. This service has been designed for rapid delivery and great value, making your sequence data available for download within 1-2 days of your samples being received. We employ streamlined technology platforms along with our proprietary reagents for an optimized and highly accurate performance, with on-hand tech support to assist with your primer design and special requirements - You can count on our dedicated and highly experienced team for a reliable service to meet all your sequencing needs.

Features/Specifications
Sequencing of templates up to 1000bp - results delivered within 1-2 days.

Submit a wide range of DNA samples:
purified PCR products
plasmids
genomic DNA
single-stranded Phage DNA
large constructs (DNA, BAC, Cosmids and YAC clones)

Select your free primers from our list of available universal primers, use our oligonucleotide synthesis service for unique primer design or send your own primers along with your samples.

We can solve many sequencing related problems, such as multiple hairpin loops and uneven base distributions.

We perform rigorous quality control checks to maintain a reliable service.

Note: For research use only

Based on prior knowledge of the region of interest, custom targeted sequencing aims to only sequence the specified subset of the genome, enabling maximal utilization of the NGS platform by giving the deepest genetic analysis compared to WGS and Exome-Seq. Depending on the list(s) of chromosomal coordinates for your target(s), scientists at abm can capture targets of up to 20 million base pairs and perform the gene sequencing for your specific project. Furthermore, special attention is paid to each project to avoid bias in accurate base calling and to detect rare alleles with sufficient sequencing depth in each analysis.

Key features for abm's custom targeted sequencing service:
flexibility to target both discrete loci as well as contiguous regions.
lower cost per sample.
faster turnaround time .
accurate results associated with the deep coverage.
base calling, read alignment, and variant calling delivered in variant call format.

Sequencing results are delivered in industry standard formats (FASTQ, SFF and BAM) with additional bioinformatic analysis available upon request and/or based on project needs. All of abm's sequencing services are performed by a group of specially trained and experienced scientists partaking in a streamlined workflow.

Note: For research use only

Based on prior knowledge of the region of interest, custom targeted sequencing aims to only sequence the specified subset of the genome, enabling maximal utilization of the NGS platform by giving the deepest genetic analysis compared to WGS and Exome-Seq. Depending on the list(s) of chromosomal coordinates for your target(s), scientists at abm can capture targets of up to 20 million base pairs and perform the gene sequencing for your specific project. Furthermore, special attention is paid to each project to avoid bias in accurate base calling and to detect rare alleles with sufficient sequencing depth in each analysis.

Key features for abm's custom targeted sequencing service:

• flexibility to target both discrete loci as well as contiguous regions.
• lower cost per sample.
• faster turnaround time .
• accurate results associated with the deep coverage.
• base calling, read alignment, and variant calling delivered in variant call format.

Targeted RNA Cancer Panel Services Available at abm:

Leukemia Cancer RNA Cancer Panel
Brain Cancer RNA Cancer Panel Service
Breast Cancer RNA Cancer Panel Service
Lung Cancer RNA Cancer Panel Service
Prostate Cancer RNA Cancer Panel Service

6 Samples $629/sample
12 Samples $450/sample
18 Samples $394/sample
24 Samples $346/sample

Comprehensive Cancer RNA Cancer Panel Service: Price on request

Sequencing results are delivered in industry standard formats (FASTQ, SFF and BAM) with additional bioinformatic analysis available upon request and/or based on project needs. All of abm's sequencing services are performed by a group of specially trained and experienced scientists partaking in a streamlined workflow.

Note: For research use only

The service uses the TruSight Cardiomyopathy panel which targets genes linked to inherited cardiomyopathies, including Hypertrophic Cardiomyopathy (HCM), Dilated Cardiomyopathy (DCM), Restrictive Cardiomyopathy (RCM), Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC), Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT), and Left Ventricular Non-Compaction (LVNC). Additional content covers several syndromes, including Danon and Fabry disease, Barth syndrome, and Transthyretin Amyloidosis — all of which can present with isolated cardiomyopathy. Data will be analyzed using VariantStudio, and the report includes a detailed explanation of all clinically-relevant variants.

The DCM, HCM, and cardiomyopathy panels target detection of mutations in 37, 31, and 56 genes, respectively, by next-generation sequencing of all coding exons plus at least 5 bases into the 5' and 3' ends of all the introns. Additional Sanger sequencing is performed for any regions missing or with insufficient read depth coverage for reliable heterozygous variant detection.

Min. Sample: 2

Note: For research use only

The Inherited Diseases Panel can aid in genetic consultation of familial disorders and potentially life-saving treatment, not only for the affected patient, but also for related family members. Benefits include:

1. Immediate treatment and long-term prognosis;
2. Medical management/intervention;
3. Specific information for genetic counseling.

Performed by next generation sequencing, this test focuses on sequencing ~4,500 genes and ~180 SNPs (single nucleotide polymorphisms) to identify the underlying mutations of the specific diseases, regardless of syndromic physiological conditions. Clinically significant variants are identified using the Burrows-Wheeler Aligner (BWA) and may be confirmed by Sanger sequencing. Each report includes a detailed explanation of all clinically-relevant variants. Min. Sample: 2

Note: For research use only

This service uses the TruSight Autism panel which includes genes reported in the Online Mendelian Inheritance in Man (OMIM) database on autism; genes with recurrent mutations resulting in developmental delays; genes with reported mutations as found in case studies involving developmental delay characteristics; and genes from summaries of autism-relevant genes. Genes neighboring strong association signals were excluded in the absence of published reports of mutations. Data will be analyzed using VariantStudio, and the report includes a detailed explanation of all clinically-relevant variants.

Min. Sample: 2

Note: For research use only

The Deafness gene panel service is performed by next generation sequencing using custom oligonucleotide-based target capture followed by Illumina sequencing of the coding regions of the DA3 panel genes; with > 100 fold coverage at every target base. Clinically significant variants are identified using the Burrows-Wheeler Aligner (BWA).

Min. Sample: 2

Note: For research use only

Comprehending the process of epigenetic modification(s) to chromatin is crucial to our understanding of core biological processes such as gene expression, maintenance of genomic integrity, parental imprinting, X-chromosome inactivation, regulation of growth and development, aging and cancer. Next Generation Sequencing has essentially revolutionized epigenetic research by making it substantially simpler, more sensitive and faster than existing methods. With such technology enabled accessibility, epigenetics can now truly begin to shed light on many common and rare physiological phenomena. Epigenetic profiling at abm serves to profile differentially methylated DNA regions associated with cancer and other diseases.

Min. Samples: 2

Note: For research use only

microRNA (miRNA) are naturally occurring, small non-protein coding sequences that are involved in post-translational gene regulation and have been implicated in a variety of biological processes and diseases. These small RNAs (~22nt) also have a big impact on many complex pathophysiological responses, making them a key part of life science research on mammalian and plant systems.
miRNA profiling allows for detection and quantification of miRNAs with high sensitivity and specificity , and provides researchers with both raw and processed data for quick interpretation and comparison of multiple samples.
miRNA Profiling Service
RNA Isolation $95.00
cDNA synthesis $75.00
Human miRNA Profiling Service (v16) $550.00
Mouse miRNA Profiling Service (v16) $550.00
Single miRNA Assay $25.00

Our profiling platform utilizes quantitative reverse transcription PCR (qRT-PCR) to achieve highly parallel and high-throughput results. Customizable profiling service is also available upon request.

Note: For research use only

Knockout up to 100 target genes with a single custom targeted sgRNA lentiviral pool. This custom product can be tailored to your specific experiments and is especially useful for the knockout of gene families and pathways. The pooled constructs and 1-2 ml of >1x10e7 IU/mL lentivirus will be provided.

Note: For research use only

Site-directed mutagenesis is a process of creating specific alterations in a gene. This technique has been used to study the relationship of protein structure and its function. Using site-directed mutagenesis, a gene can be modified by base substitution, sequence deletion and insertion. Site-directed mutagenesis is also very useful for modifying a DNA construct, such as alteration of a restriction site in a vector, insertion or deletion of vector elements, tag addition, tag removal and promoter insertion and exchange. Please inquire for a quote for large plasmids, large fragment insertions/deletions, difficult templates, and multiple mutagenesis.

Features

• perform PCR-based mutagenesis on up to 25bp (consecutive) within a <1kb fragment in an existing abm product or a provided vector
• full length sequencing to confirm presence of the mutated region
• deliver plasmid DNA
• turn-around time: 10-15 business days for orders of 2ug of DNA

Note: For research use only

abm offers an extensive library of sequence verified human, mouse and rat open reading frames (ORF), ready for insertion into your choice of protein expression systems. With both the 5'UTR and the 3'UTR removed, ORFs allow for quick and reliable protein expression when inserted into a compatible expression vector. They can also be used as a template for positive control in PCR amplification experiments.

abm's ORFs contain a mutated stop codon to allow for the incorporation of a C-terminal tag or reporter if it is present in the recipient vector. abm's ORFs are also available in ready-to-use protein expression vectors, lentiviral vectors or packaged lentivirus and adenovirus formats.

Note: For research use only

iPSC generation is a complicated cellular process, which is a time-consuming and technically challenging technique. Scientists at abm have developed streamed-line protocols for efficient iPSC generation with episomal plasmids, viral vectors, and recombinant proteins.With 100% success rate, abm is guaranteed to provide highly efficient reprogramming of somatic cells into iPSC with our lentiviral vectors. Custom transgene-free, footprint-free iPSC generation (at 80% success rate) is also available with our non-integrating system, providing safety for the iPSC to be used in drug discovery and cell therapy applications. Molecular and functional assays such as flow cytometry, karyotyping, and tetratoma formation are often used to characterize the pluripotency of the newly established iPSC cell line.

Custom iPSC Generation Services
iPSC Generation from Human Peripheral Blood or Fibroblast by Lentiviral Vectors $5,000.00
Integration-Free iPSC Generation by mini-Circle DNA $18,750.00
Integration-Free iPSC Generation by Proteins $25,000.00
Integration-Free iPSC Generation from Human Peripheral Blood by Episomal Plasmids $4,500.00
Integration-Free iPSC Generation from Human Fibroblast by Episomal Plasmids $5,000.00

Custom ES/iPSC Characterization Services
Teratoma Formation Analysis $2,800.00
Karyotyping by G-banding $950.00
Pluripotency Marker Characterization by Flow Cytometry $450.00

Each of these services will be provided with a comprehensive report suitable for publications.

Note: For research use only

Because of our expertise in cell and stem cell biology, scientists at abm have developed unique media indispensible for iPSC, ESC and MSC research.

Stem Cell Media Products
Human Pluripotent Stem Cell Culture Medium Kit (500 ml) $150.00
Rat Pluripotent Stem Cell Culture Medium Kit (500 ml) $200.00
Mesenchymal Pluripotent Stem Cell Culture Medium Kit (500 ml) $175.00
Neural Stem Cell Culture Medium Kit (500 ml) $150.00

Note: For research use only

Primary cells are taken directly from living tissue (e.g. biopsy material) and cultured in vitro. As opposed to continuous cell lines (e.g. tumor), primary cells are known for their virtue of retaining most of the characteristics of the original tissue from which they are derived in the first place.

abm is one of the global leaders in primary cells. A result of years of in-house research and development and cooperative endeavours with prestigious research institutions worldwide, abm has successfully accumulated a large number of primary cells to provide for a wide-selection of research projects for our clients.

Note: For research use only

abm has over 10 years of experience generating stable cell lines expressing your gene of interest using our vast array of expression systems. Our established and streamlined process provides from vector construction and virus packaging to cell screening and QC.

Key Service Features:

• Lentivirus packaging
• Clone screening by antibiotic selection
• Clone selection by gene expression evaluation
• Clonal expansion and cryopreservation

Deliverables:

• Up to 1 clone, 2 vials/clone
  Service Report includes:
• Morphology Assessment
• qPCR Analysis on the Transgene expression
• Test results for the presence/absence of microbial contaminants, including bacterial and fungal testing.
• GFP/RFP/Luciferase test if reporter is present

Add-on Service:

• Gene Expression Assay Service: $135.00
• Additional Clones: $250.00
• Additional Vials of Delivered Clones: $80.00
• HA Tag Validation (Up to 10 Clones): $150.00
• Other Validation Service by Western Blot (Up to 10 Clones)**: $975.00
• STR profiling of 1 WT Parental Cell line & 1 Stable Cell Line Clone: $150.00
• Contaminant Detection - Mycoplasma Spp.: $52.00

Notes:

• A non-refundable 15% deposit is required to initate the project
  ** Customer to provide validated antibody

abm's Adeno-Associated Virus (AAV) is an attractive tool for custom tissue specific gene delivery and long term transient expression in proliferating and quiescent cells. Featuring: sgRNA expressing AAV available for CRISPR Knock-out!

Key Service Features:

• Tissue specific delivery with 10 AAV serotypes
• Long-term expression of non-integrated gene
• Able to transduce proliferating and quiescent cells
• Low immunogenicity and pathogenicity in vivo
• Package up to 4Kb of insert

Applications:

• Long-term gene expression in mammalian systems.
• siRNA AAV for efficient gene expression knock-down.
• sgRNA AAV for gene knock-out.

Custom AAV production service (without plasmid amplification):

• AAV production and purification to 10^9 GC/ml: $550.00
• AAV production and purification to 10^12 GC/ml: $950.00
• AAV production and purification to 10^13 GC/ml: $2500.00

Custom AAV production service (with plasmid amplification):

• AAV production and purification to 10^9 GC/ml: $750.00
• AAV production and purification to 10^12 GC/ml: $1150.00
• AAV production and purification to 10^13 GC/ml: $2700.00

Custom AAV plasmid cloning service:

• AAV plasmid cloning with compatible insert: $350.00
• AAV plasmid cloning without compatible insert: $450.00

Custom siRNA AAV production service:

• Custom AAV siRNA Pooled Vector 1.0 ug: $395.00
• AAV siRNA Pooled Virus: $795.00

Custom sgRNA AAV plasmid cloning service:

• spCas9 1 x sgRNA AAV vector: $135.00
• spCas9 3 x sgRNA AAV vector: $335.00
• saCas9 1 x sgRNA AAV vector: $135.00
• saCas9 3 x sgRNA AAV vector: $335.00
• saCas9 1 x All-in-One AAV vector: $185.00
• saCas9 3 x All-in-One AAV vector: $385.00

Titer Upgrades for Existing AAV Viruses Services:

• Upgrade from 10^9 AAV Virus to High Titer AAV Virus (10^12 GC/ml): $400.00
• Upgrade from 10^9 AAV Virus to High Titer AAV Virus (10^13 GC/ml): $1200.00

Custom AAV Titration:

• AAV Titer Assay service via qPCR: $75.00

Note: For research use only

abm's lentivirus is a powerful tool for stable transgene expression in dividing and non-dividing cells. Our proprietary packaging mix and protocol allow rapid production of lentiviral particles up to 10^10 IU/ml. Featuring: sgRNA lentivirus for gene knock-out!

Key Service Features:

• Stable integration of transgene up to 4.5kb
• High titer production
• Proven quality of numerous publications

Applications:

• Stable transgene expression in mammalian systems
• sgRNA lentivirus for gene knock-out

Custom Lentivirus Production without plasmid amplification:
Custom Recombinant Lentivirus (10^6 IU/ml; 10 ml) / $450.00
High-Titer Custom Recombinant Lentivirus (10^7 IU/ml; 1.0 ml)/ $700.00
High-Titer Custom Recombinant Lentivirus (10^8 IU/ml; 550 μl)/ $950.00
High-Titer Custom Recombinant Lentivirus (10^9 IU/ml; 100 μl)/ $1200.00
High-Titer Custom Recombinant Lentivirus (10^10 IU/ml; 100 μl)/ $1650.00

Custom Lentivirus Production with plasmid amplification:
Custom Recombinant Lentivirus (106 IU/ml; 10 ml)/ $650.00
High-Titer Custom Recombinant Lentivirus (10^7 IU/ml; 1.0 ml)/ $850.00
High-Titer Custom Recombinant Lentivirus (10^8 IU/ml; 550 μl)/ $1100.00
High-Titer Custom Recombinant Lentivirus (10^9 IU/ml; 100 μl)/ $1350.00
High-Titer Custom Recombinant Lentivirus (10^10 IU/ml; 100 μl)/ $1850.00

Custom sgRNA Lentivirus production:
Custom sgRNA Lentivirus (1 target): $395.00
Custom sgRNA Lentivirus set (3 target-set): $895.00
Custom sgRNA All-in-One Lentivirus (1 target): $495.00
Custom sgRNA All-in-One Lentivirus set (3 target-set): $1175.00
Note: We guarantee gene knock-out using our 3 target-set.

Custom Lentiviral Plasmid Cloning Services:
Sub-cloning gene of interest (with compatible sites) into a lentiviral vector system/ $550.00
Sub-cloning gene of interest (without compatible sites)into a lentiviral vector system/ $725.00
PCR-cloning gene of interest (<3.0kb) into a lentiviral vector system/ $550.00
PCR-cloning gene of interest (>3.0kb) into a lentiviral vector system/ $725.00

Custom Lentivirus Titration:
qPCR Based (Detectable Range of 10^4 - 10^10 IU/ml)/ $68.00

Note: For research use only

abm produces custom recombinant adenovirus containing full-length cDNA, allowing high level gene expression in host cells or RNAi expression for efficient elimination of target gene expression.

Key Features:

• High titer production up to 10^12 pfu/ml
• High gene expression level
• Broad tropism
• Package genes up to 8Kb!

Custom Adenovirus Production Service:
Recombinant Adenovirus production (10^6 pfu/ml): $650.00

Custom Adenovirus Plasmid Service:
Cloning Insert containing plasmid into pShuttle with Compatible Sites: $350.00
Cloning Insert containing plasmid into pShuttle without Compatible Sites: $450.00
Clone <2kB Insert into pShuttle: $550.00
Clone 2-4kB Insert into pShuttle: $650.00
Clone >4kB Insert into pShuttle: $750.00

Custom Adenovirus Purification from seed stock:
Amplify and purify (2-3 ml of 10^9 pfu/mL): $450.00
Amplify and purify (2-3 ml of 10^10 pfu/mL): $550.00
Amplify and purify (5 x 200 μl of 10^11 pfu/mL): $850.00
Amplify and purify (5 x 200 μl of 10^12 pfu/mL): $1550.00

Adenovirus Titer Assay Service:
Plaque Assay: $450.00
RCA Assay: $450.00
TCID50 Assay: $350.00

Note: For research use only.

abm's custom retrovirus production is a highly efficient approach to deliver genes of interest for stable expression into a broad range of dividing mammalian cells. Our custom retrovirus production is a proven method to get accurate and precise gene expression in your cell population.

Retrovirus plasmid cloning service:
Retrovirus Subcloning Service/ $550.00

Retrovirus production service:
(customer providing enough DNA)
Custom Retrovirus 10 ml of 10e5 IU/ml (50 μg of DNA Needed) / $450.00
Custom Retrovirus 10 ml of 10e6 IU/ml (50 μg of DNA Needed) / $700.00
Custom Retrovirus 1.0 ml of 10e7 IU/ml (350 μg of DNA Needed) / $950.00
Custom Retrovirus 1.0 ml of 10e8 IU/ml (350 μg of DNA Needed)/ $1650.00
Custom Retrovirus 100 μl of 10e9 IU/ml (700 μg of DNA Needed)/ $2500.00

(DNA amplification required)
Custom Retrovirus 10 ml of 10e5 IU/ml/ $600.00
Custom Retrovirus 10 ml of 10e6 IU/ml/ $850.00
Custom Retrovirus 1.0 ml of 10e7 IU/ml/ $1100.00
Custom Retrovirus 1.0 ml of 10e8 IU/ml/ $1800.00
Custom Retrovirus 100 μl of 10e9 IU/ml/ $2700.00

Note: For research use only.

Contamination of cell cultures with mycoplasma is a widespread laboratory problem that can jeopardize important experimental results. abm offers the following services to help you resolve this issue.

Mycoplasma Detection Service Details:
Cell culture samples sent in by the customer will be analyzed for mycoplasma contamination and a report will be provided within 10 business days of receiving the cells.

Mycoplasma Elimination Service: $1500.00/ cell line
Mycoplasma-contaminated cell cultures sent in by the customer will be treated with anti-mycoplasma cocktails and delivered back to the customer once elimination has been confirmed. A report will be provided with the cell culture to certify the success of the treatment. The entire process will be completed within approximately 45 business days of receiving the cell culture.

Note: For research use only.

Escherichia coli (E. coli) provides for a relatively simple, fast, inexpensive and robust system for native and heterologous recombinant protein production. While unable to render many of the complex eukaryotic post-translational modifications, technologies such as, co-expression of chaperones/kinases/rare tRNAs and engineering of strains that provide a pro-disulfide bond (oxidative) environment, hallmark the technological progress achieved in E. coli system over the last two decades. abm recommends the E. coli system when post-translational modifications such as glycosylation are not crucial to the intended downstream application(s).

PHASE I services
Cloning/sub-cloning of gene of interest into a protein-encoding gene expression vector
Sequence confirmation/ $950/ (3-5 weeks)

PHASE II services*
A set of multivariate small scale protein production trials are conducted to test for yield, solubility and optimal growth conditions/ $625/ (2 weeks)

PHASE III services*
Large scale (1mg*) recombinant protein production and purification (purity ≥90%)
*For >1mg of recombinant protein, please contact our customer support./ $1150/ (Up to 3 weeks)
Proteolytic removal of affinity tag/ $500/ (+2 days)
Special Buffer Request/ $350.00
Special Concentration Request/ $350.00
Lyophilization Request / $00.00

Custom protein production in Mammalian cells
The mammalian expression system provides the only authentic and feasible option in cases where the protein of interest needs to be embellished with characteristic, mammalian post-translational modification(s) and/or entails a complex translational machinery for proper folding and targeting (such as membrane proteins). Therefore, abm recommends its mammalian expression service for proteins for which the native folding and post-translational modifications are of a complex nature (beyond the purview of E. coli ) and indispensable to the customer's intended application (s).

PHASE I services*
Cloning/sub-cloning of gene of interest into a protein-encoding gene expression vector
Sequence confirmation/ $950/ (4-6 weeks)

PHASE II services*
Generation of stable protein producing cell-line(s)/ $1050/ (4 weeks)

PHASE III services*
Production of crude lysate/ $850/ (2 weeks)

PHASE IV services*
Large scale recombinant protein production and purification. Yield: 5-50µg, purity ≥ 90%/ $1150/ (3 weeks)
Proteolytic removal of affinity tag/ $600/ (+3 days)

Special Buffer Request / $350.00
Special Concentration Request / $350.00
Lyophilization Request/ $100.00

Note: For research use only

Key requirements for successful monoclonal antibody generation include immunization strategies, antigen design, and screening technologies. abm's novel immunization procedures and careful mouse strain selection allows for production of monoclonal antibodies which are of the utmost affinity and specificity. This is because we take great care in designing the antigens for antibody production, especially with respect to synthesizing peptides, to generate a robust antibody that is able to recognize conformational changes of the native protein under the optimum number of conditions. Mouse monoclonal antibody production is divided into different phases, with a non-refundable $1,000 to cover the material cost to initiate the project. The balance will be due upon service completion. Our price includes antigen protein production and guaranteed western blot results, which are not included in competitor's services. All you need to do is give us the gene accession number and we will take care of everything else. This unique service is only available from abm.

Complete Package Price: $5000, includes the following:

1. Antigen production
2. Immunization (5 mice/antigen) and Sera Testing by ELISA
3. Fusion and Colony Screening
4. Subcloning, Colony Expansion and Freezing
5. Guaranteed western blot positive clones (2 clones)
6. Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)

Package without protein production Price: $3200, includes the following:

1. Immunization (5 mice/antigen) and Sera Testing by ELISA
2. Fusion and Colony Screening
3. Subcloning, Colony Expansion and Freezing
4. Guaranteed western blot positive clones (2 clones)
5. Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)

Additional Clone(s)
Steps 4 and 5: $500.00 per Clone
Ascites Production Production from 1-25 Mice: $15.00 per Mouse
Production from 26-50 Mice: $12.50 per Mouse
Production from 51-100 Mice: $10.00 per Mouse
Production from 101-150 Mice: $9.50 per Mouse
Production from 151-200 Mice: $9.00 per Mouse
Production from 200+ Mice: $8.00-22.50 per Mouse

Custom Polyclonal Antibody Production
abm offers the most compeptitive price for quality, custom, polyclonal antibody production. Our services include peptide synthesis, immunization of 2 rabbits, and peptide affinity purification of positive serum. It is all included and there is absolutely no hidden additional cost. Based on our experience, the success rate of generating a quality polyclonal antibody is over 70% with one peptide antigen and over 95% for two peptide antigen.

Standard Service Price: $950 per antigen

1. Customer provides antigens (5mg of >90% purity peptide or 5mg recombinant protein).
2. KLH conjugation and immunization of 2 rabbits (most service providers charge an extra $100.00 for KLH conjugation).
3. ELISA assay for titer until it reaches 1:10,000 or better.
4. Peptide affinity purification (2-3ml purified antibody), most service providers charge an extra $500.00 for this step.
5. The project will be finished in 12 weeks.

Complete Service Price: $1250 per antigen

1. Customer provides gene name or gene/protein sequence.
2. Peptide is designed and synthesized.
3. KLH conjugation and immunization of 2 rabbits (most service providers charge an extra $100.00 for KLH conjugation).
4. ELISA assay for titer until it reaches 1:10,000 or better.
5. Peptide affinity purification (2-3ml purified antibody), most service providers charge an extra $500.00 for this step.
6. The project will be finished in 14 weeks.

Note: For research use only

abm's IMPACT test examines the presence of microbial contamination in biological samples used for cell or animal research. abm's advanced testing methodologies is superior to the traditional Mouse Antibody Production (MAP) test and produces results in 5 days.

Key features:

• Highly sensitive to detect smaller amount of infectants
• Does not require the use of live animals
• Does not rely on mouse immune response
• Short turn-around-time of 5 days

Deliverables:

• Full test report

IMPACT Service I: $425.00 per sample

• Mycoplasma Spp
• Sendai Virus (SeV)
• Mouse Hepatitis Virus (MHV)
• Pneumonia Virus of Mice (PVM)
• Minute Virus of Mice (MVM)
• Mouse Parovirus (MPV-1, MPV-2, MPV-3)
• Theiler's Murine Encephalomyelitis Virus, GDV-II Strain
• Murine Norovirus (MNV)
• Reovirus Type 3 (Reo 3)
• Mouse Rotavirus (EDIM)
• Ectromelia Virus (ETCV)
• Lymphocytic Choriomeningitis Virus (LCMV)
• Murin Polyoma Virus (Py)
• Lactate Dehydrogenase-elevating Virus (LDEV)
• Mouse Adenovirus (MAd1, MAd2)
• Mouse Cytomegalovirus (mCMV)
• K Virus (KV)
• Hantaan Virus (HV)

IMPACT Service II: $365.00 per sample

• Mycoplasma Spp
• Sendai Virus (SeV)
• Mouse Hepatitis Virus (MHV)
• Pneumonia Virus of Mice (PVM)
• Minute Virus of Mice (MVM)
• Mouse Parovirus (MPV-1, MPV-2, MPV-3)
• Theiler's Murine Encephalomyelitis Virus, GDV-II Strain
• Murine Norovirus (MNV)
• Reovirus Type 3 (Reo 3)
• Mouse Rotavirus (EDIM)
• Ectromelia Virus (ETCV)
• Lymphocytic Choriomeningitis Virus (LCMV)
• Murin Polyoma Virus (Py)
• Lactate Dehydrogenase-elevating Virus (LDEV)
• Mouse Adenovirus (MAd1, MAd2)
• Mouse Cytomegalovirus (mCMV)

IMPACT Service III: $335.00 per sample

• Mycoplasma Spp
• Sendai Virus (SeV)
• Mouse Hepatitis Virus (MHV)
• Pneumonia Virus of Mice (PVM)
• Minute Virus of Mice (MVM)
• Mouse Parovirus (MPV-1, MPV-2, MPV-3)
• Theiler's Murine Encephalomyelitis Virus, GDV-II Strain
• Murine Norovirus (MNV)
• Reovirus Type 3 (Reo 3)
• Mouse Rotavirus (EDIM)
• Ectromelia Virus (ETCV)
• Lymphocytic Choriomeningitis Virus (LCMV)
• Murin Polyoma Virus (Py)
• Lactate Dehydrogenase-elevating Virus (LDEV)

IMPACT Service IV: $175.00 per sample

• Mycoplasma Spp
• Sendai Virus (SeV)
• Mouse Hepatitis Virus (MHV)
• Pneumonia Virus of Mice (PVM)
• Minute Virus of Mice (MVM)
• Mouse Parovirus (MPV-1, MPV-2, MPV-3)
• Theiler's Murine Encephalomyelitis Virus, GDV-II Strain

Custom your own IMPACT Services: $52.00 per test

• Mycoplasma Spp.
• Sendai Virus (SeV)
• Mouse Hepatitis Virus (MHV
• Pneunomia Virus of Mice (PVM)
• Minute Virus of Mice (MVM)
• Mouse Parovirus (MPV-1, MPV-2, MPV-3)
• Theiler's Murine Encephalomyelitis Virus, GDV-II Strain
• Murine Noravirus (MNV)
• Reovirus Type 3 (Reo 3)
• Mouse Rotavirus (EDIM)
• Ectromelia Virus (ETCV)
• Lymphocytic Choriomeningitis Virus (LCMV)
• Murine Polyoma Virus (Py)
• Lactate Dehydrogenase-elevating Virus (LDEV)
• Mouse Adenovirus (MAd1, MAd2)
• Mouse Cytomegalovirus (mCMV)
• K Virus (KV)
• Hantaan Virus (HV)

Sample Requirements:
Biological Samples/Cell Culture
Please send in 1 cryovial per sample containing at least 2x10^6 cells/vial. We accept samples in cell pellet form, in growth medium, freeze medium or phosphate-buffered saline.

Liquid Samples
Please send in 1 cryovial per sample with a minimum volume of 0.5ml for best results. However, if sample is limited, smaller amounts can also be accommodated. Please contact us for details.

All samples should be collected under sterile conditions.

Product and services intended for research use only

abm is proud to offer a wide-repertoire of recombinant human growth factors and cytokines that offer (a) authentic biological activity (b) < 1.0 EU of endotoxin/µg of protein as estimated by the LAL method (c) ≥ than 95% purity and (d) option for customizable filling/aliquot size. Each of abm's growth factor/cytokine product batch is backed by comprehensive quality control.

Recombinant BMP2 (10 µg) at $70.00
Recombinant Human G-CSF3 (10 µg) at $70.00
Recombinant Human or Mouse LIF (10 µg) at $70.00
Recombinant Human TNF (50 µg) at $70.00
Recombinant Mouse TNF (50 µg) at $70.00
Recombinant Rat TNF (50 µg) at $70.00
Recombinant Human IL-6 (20 µg) at $70.00
Recombinant Mouse IL-6 (10 µg) at $70.00
Recombinant Rat IL-6 (10 µg) at $70.00

Note: For research use only

abm is a leading company in custom peptide synthesis. Through innovation, abm has developed a variety of proprietary technologies that allow us to provide the highest quality custom synthetic peptides at the most competitive prices. Our custom peptide synthesis offers the following advantages:

1. No Length Limit
2. High Quality
3. Competitive Pricing
4. Flexibility
5. Fast Delivery Time
6. Custom Peptide Modifications available

Peptide Synthesis Service
Crude (Purity):
Starting at $4.14 (for 1-4 mg synthesis), up to 1000 mg production at $37.08

Desalted (Purity):
Starting at $5.31 (for 1-4 mg synthesis), up to 1000 mg production at $38.61.

75% Purity:
Starting at $8.91 (for 1-4 mg synthesis), up to 1000 mg production at $88.47.

85% Purity:
Starting at $11.88 (for 1-4 mg synthesis), up to 1000 mg production at $105.75.

90% Purity:
Starting at $14.22 (for 1-4 mg synthesis), up to 1000 mg production at $127.08.

95% Purity:
Starting at $15.21 (for 1-4 mg synthesis), up to 1000 mg production at $141.12.

98% Purity:
Starting at $22.68 (for 1-4 mg synthesis), up to 1000 mg production at $211.17.

Notes:
*The minimum charge per peptide is $150.
*The above prices are per amino acid in the peptide and includes the cost for purification and mass spectrum analysis.
*There are no hidden charges.
*Please contact us for large scale peptide synthesis quote (from grams to kilograms).

Note: For research use only

At abm we have a breadth of experience in purifying plasmid DNA. As a result, we offer high quality custom nucleic acid and plasmid DNA purification services of Transfection Grade for mammalian cells or Ready-to-Inject for transgenic applications.

Plasmid Purification Service
Endo-toxin Free, High Quality Plasmid Purification for up to 1.0 mg $195.00/mg
Endo-toxin Free, High Quality Plasmid Purification for 2-4 mg $145.00/mg
Endo-toxin Free, High Quality Plasmid Purification for 5-9 mg $125.00/mg
Endo-toxin Free, High Quality Plasmid Purification for 10-20 mg $109.00/mg
Endo-toxin Free, High Quality Plasmid Purification for 21-40 mg $99.00/mg
Endo-toxin Free, High Quality Plasmid Purification for 41-60 mg $89.00/mg
Endo-toxin Free, High Quality Plasmid Purification for 60-10 0mg $79.00/mg

Note: For research use only

abm offers custom Herpes Simplex Virus (HSV-1) Virus production that can accommodate large gene inserts and allow lifelong infection to achieve high level gene expression in host cells, particularly in neurons.

Key Features:

• Delivery of large transgenes
• Non-integrating, but can replicate separately from host cell genome.
• Establish lifelong non-toxic latent infections in neurons and establish stable transgene expression
• Higher titers yields and lower toxicity

Custom HSV Virus Production:
Custom HSV Virus Production (10^6 IU/ml): $750.00 / 10ml
Custom HSV Virus Production (10^7 IU/ml): $1,550.00 / 2 x 200μl

HSV Virus Controls:
HSV GFP Control Virus (10^6 IU/ml): $695.00 / 10ml
HSV GFP Control Virus (10^7 IU/ml): $1495.00 / 2 x 200μl

Custom HSV Plasmid Cloning:
Custom HSV Plasmid Cloning with Compatible Insert (up to 3kb): $550.00
Custom HSV Plasmid Cloning without Compatible Insert (up to 3kb): $650.00
Additional Charge for Extra 1kb: $100.00

Insert synthesis Service ($0.18/bp, minimum charge $99.00)

Note: For Research Use Only

abm's custom CRISPR knock-out cell line service guarantees KO in your target of interest in a homogenous cell population on any of our immortalized cell line or a custom cell line of your choice.

Key Service Features:

• Risk-free service! Make no payment until KO has been confirmed.*
• Select from any of the listed cell lines (pre-determined easy to transfect cell lines)**
• sgRNA vector design, construction & delivery into cells
• Clonal screening and selection
• Screening & validation of KO by Sanger sequencing
• Expansion & cryopreservation of selected clone(s)
• PCR based Mycoplasma testing

Deliverables:

• Sequence verified KO cell line (at least 1 clone, 2 vials per clone)
• Mycoplasma tested and a Service Report

Notes:

• Non-refundable 15% deposit required to initiate the project
  ** Choose from HEK293, HEK293T, A549, HeLa, MDCK, A375, HepG2, HT1080 or U87MG. These cell lines are available as Cas-9 expressing versions or parental version. Cas9 expressing version will be used by default.If the parental version is preferred, please request prior to order placement.
  *** A signed MTA is required prior to cell shipment

Cell and Tissue Culture Services