Founded in 2011, Antibody Design Labs goal is becoming a centralized place to access tools, information, techniques in the field of recombinant antibody generation, engineering and design. Our catalog includes a rich line of phage display products and tools for recombinant antibody expression and new products are added regularly. We also offer multiple services in antibody engineering; by using our products directly accessible on our catalog, we have created a unique offering in the field with no dark zone and clear access to clones, samples, controls and associated technologies.
As one of the leaders in phage display technology we will provide expertise and knowledge on your phage display project, advise you with vector choice, screening strategies (hapten, cell surface, protein, peptide) and methods to assay the binding and convert phage clones rapidly into useful antibody formats, including Fab fragments, full IgGs, Fc fusions, etc. Several advanced platforms including Fab library, peptide libraries, and immunized libraries with in particular our llama and our rabbit platforms can be accessed through this service.
We offer phage library screening in conjunction with our phage library constructions services. Please, inquire with your particular project.
Phage Antibody Library Construction:
Fab libraries with diversity of 1E8 and above can be built on demand with 80% and more functionality. High affinity antibodies, similar to antibody generated by hybridoma technology, are isolated from them by straightforward selection techniques. We have extensive experience in building phage antibody libraries in varied formats and display types. You provide the antigen; we conduct the immunization, design and build the antibody library for you as a service.
Murine antibody libraries.
Phage display enables very selective screens to isolate clones that are not always accessible by classical hybridoma technology. We will immunize five mice with your antigen; the spleen of the best responder will be harvested and used to amplify cDNA and build the library. We can provide you with the bleeds to make your own testing.
Custom Rabbit Monoclonal Antibodies by Phage Display
Rabbits are a great source of antibodies; there are outbred animals mounting very strong immune responses to most antigens, including many antigens that do not elicit a significant response in inbred laboratory mice and small haptens in particular and constitute a good alternative to mice when monoclonal antibodies are needed. We will immunize two rabbits with your antigen; the spleen of the best responder will be harvested and used to amplify cDNA and build the library. We can provide you with the bleeds to make your own testing.
Antibody Format & Library Size
We have developed straightforward methods to build Fab libraries from varied immune sources. Our service will guarantee a minimum of 1E8 Fab clones with identical diversity on both VH and VL arms. This is particularly desirable for rabbit libraries because of the extensive contribution of VL CDR3 diversity to the high affinity of rabbit IgG antibodies. We can increase the diversity
Phage Peptide Library Construction Service:
Phage display peptide libraries have become a major drug discovery tool to identify de novo peptide ligands and explore sequence space in proteins. With more than 20 years of experience in the field, we are offering our expertise in the construction of peptide libraries on phage as a service for your research and development efforts.
We are a major provider of phage peptide libraries to the industry since many years; our libraries are built on full phage and phagemid vectors with diversity ranking from 1E8 to above 1E10, with on average between 1 and 5 billion clones delivered per library. We can accommodate multiple strategies to introduce diversity, from degenerated NNK codons to trimer codon technology, including randomization strategies to control amino acid diversity. We welcome all projects and and will be happy to discuss strategies to build complex libraries.
Vector Design & Display Type
A large choice of vectors is available to display your library in varied formats and types:
Codon Design and Mutagenesis Strategy
We carry several methods to control the amount of diversity introduced at each position of the sequence and can mixed them to optimize the quality of your library. A limited repertoire with a well represented sequence space is always better than very large, degenerated libraries containing high levels of redundant codons and junk sequences with stop codons and unpaired cysteines leading to unwanted dimers. We will assist you to choose the methods that best suit your need.
The size of the library will be tailored to your project. We routinely build libraries between 1E8 and 1E10 transformants depending on the number of variable positions and the degree of sequence space to be analyzed. Our standard library size is 10(8) in-frame clones.
Single domain antibody / sdAb / VHH / nanobody / camelid antibody
sdAb or single domain antibodies, also named VHH or nanobodies, are antibodies made of one single heavy chain domain. They are naturally found circulating in the blood of camelids (llama, camel, etc.).
sdAb are remarkable by their unique physicochemical properties in the antibody world: there are single chain, small (12-15 kD), highly soluble, extremely stable and usually express at very high yields in any systems. Camelid sdAb are structurally close to human antibody of the VH3 family, making humanization an easy process.
Antibody Design Labs offers immunization, phage library construction and screening services. We can build a complete library from as little as 4 ml of llama blood. Please, contact us for your specific project.
Getting accurate sequence of the variable domains of your monoclonal antibodies will open up a realm of possibilities for downstream antibody engineering from in vitro recombinant expression to affinity maturation and humanization. Knowing the sequence of your antibodies will protect you from the loss of your valuable clones; it is one of the first steps to get protection of your antibodies through patent applications and it is an important quality control to maintain consistent cell lines for batch production.
Antibody Design Labs offers a fast, reliable and competitively-priced sequencing service to clone your antibody cell line. Both light chain and heavy chain variable domain cDNAs will be amplified using either a combination of leader primers or state-of-the-art rapid amplification of DNA ends (5’-RACE). A full length sequencing service including complete leader sequence using 5’-RACE and sequence of constant domains (full antibody) is also available upon request. We can sequence from a variety of species including mouse, rat, rabbit and hamster hybridomas or human EBV-immortalized B-cell lines and isotypes including IgM & IgA. Hybridomas are prone to contain multiple chains as a result of cellular fusions involving multiple B-cells (quadromas), non-productive rearrangements generated during B-cell maturation and pseudogenes; we will conduct a thorough and extended analysis of multiple clones to confirm the identity of the relevant full-length, in-frame sequences and eliminate aberrant endogenous myeloma light chains. This is a “No Success – No Fee” service; you pay on delivery of your antibody sequence.
A detailed submission protocol will be provided. Information on species and isotype will greatly accelerate turnaround time. You need to ship between 1 and 5×10(6) cells per hybridoma either as a flash-frozen cell pellet on dry ice or as RNA-stabilized cells at room temperature (we provide containers with RNA stabilization reagent upon request). We accept smaller samples 1×10(5) and less and can help you to recover the sequence of dead hybridomas.
We welcome discussing the result directly with you.
Cost & Turnaround Time
See table above for starting service fees & expected turnover.
We provide high-quality cloning service for your antibody in the format that best suit your needs: scFv, scFv-Fc fusions, Fab, Fab’2, full-length IgG and more. Switch variants and chimeric antibodies can be produced in varied isotypes of the species of your choice, including human IgM, IgA, IgG1, IgG3 and IgG4 with either kappa or lambda light chain and mouse equivalents. More specialized constructions are welcome such as Fc or albumin fusions and minibodies.
Antibody Design Labs offers an express transient expression service using a robust platform based on our set of TEGX™ mammalian expression vectors. We employ CHO and HEK293 cells in suspension culture and serum-free conditions to achieve high levels of expression in just a few days.
A service adapted to your needs
Collection of starting material
A hybridoma sequence (see our hybridoma sequencing service), a phage clone, a scFv or a Fab sequence, a sequence from your repository or simply a GenBank accession number is required to start the project.
Antibody Design Labs has not received any reviews.
Antibody Design Labs has not received any endorsements.