1321777763000

Antibody and Protein Resource

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Nashville, Tennessee, US

About Antibody and Protein Resource

Divison of: Vanderbilt University

The Vanderbilt Antibody and Protein Resource (VAPR) facility offers technical advice and services aimed at simplifying the process of generating, purifying, and characterizing monoclonal and polyclonal antibodies. It combines and replaces the Vanderbilt Monoclonal Antibody Core (VMAC) and the Protein Expression... Show more »

The Vanderbilt Antibody and Protein Resource (VAPR) facility offers technical advice and services aimed at simplifying the process of generating, purifying, and characterizing monoclonal and polyclonal antibodies. It combines and replaces the Vanderbilt Monoclonal Antibody Core (VMAC) and the Protein Expression User Club (PEUC).

We also offer recombinant protein production and purification services in both bacterial and mammalian expression systems.

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Our Services (21)


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Antibody Isotyping

Starting at $30.00 per sample

FAST method

FAST method

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Therapeutic Monoclonal Antibody (mAb) Development

Starting at $7,000.00 per sample

2 Rabbits included
Includes all costs for 59 day protocol. Extensions available.
Collection and ELISA screening of sera
Optional affinity purification of antibodies available
only one antigen will be created and utilized

2 Rabbits included
Includes all costs for 59 day protocol. Extensions available.
Collection and ELISA screening of sera
Optional affinity purification of antibodies available
only one antigen will be created and utilized

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Antibody Purification

Starting at $977.00 per sample

Large-scale monoclonal antibody production and purification (generally obtain 20-80mg purified antibody)

Scale-up and Purification $977

Purity/Quantitation assessment
A280 Method price included
BCA Assay Method
1st sample $30
each additional sample $9
Gel Method (preferred method) $106

Protein G... Show more »

Large-scale monoclonal antibody production and purification (generally obtain 20-80mg purified antibody)

Scale-up and Purification $977

Purity/Quantitation assessment
A280 Method price included
BCA Assay Method
1st sample $30
each additional sample $9
Gel Method (preferred method) $106

Protein G purification of antibodies from supernatant
Purification $411 / liter
Purity/Quantification Assessment as above

Affinity Purification of polyclonal antibodies
Column Preparation $412
Purification $497
Purity/Quantitation assessment as above

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Cell Line Authentication

Starting at $165.00 per sample

required for all lines entering the facility $165 / cell line

required for all lines entering the facility $165 / cell line

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Cryostorage and Cryoprocessing

Starting at $3.00 per sample

Cryo-storage of hybridoma Line $3 per month / cell line
Cryopreservation of hybridoma Non Fusion Based $55 / cell line

Cryo-storage of hybridoma Line $3 per month / cell line
Cryopreservation of hybridoma Non Fusion Based $55 / cell line

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ELISA

Enzyme-linked immunosorbent assay
Starting at $104.00 per sample

Monoclonal antibody isotyping

1st clone $104
each additional clone $12

Monoclonal antibody isotyping

1st clone $104
each additional clone $12

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Peptide Synthesis

Price on request

contact for pricing

contact for pricing

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Polyclonal Antibody Production

Starting at $8.00 per sample

This project format is designed to maximize the generation of high quality antibodies. We have combined all of the factors we have found to positively impact the isolation of novel and useful antibody reagents.

One of the key features is the generation of antigens to two or more antigenic regions of the target protein (amino... Show more »

This project format is designed to maximize the generation of high quality antibodies. We have combined all of the factors we have found to positively impact the isolation of novel and useful antibody reagents.

One of the key features is the generation of antigens to two or more antigenic regions of the target protein (amino and carboxy termini if appropriate). These antigens will then be co-immunized and screening will be conducted for antibodies targeting both antigens.

2 Rabbits included

Includes all costs for 59 day protocol. Extensions available.

Collection and ELISA screening of sera

Optional affinity purification of antibodies available

Cost = approximately $8,500

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Bioconjugation/Chemical Crosslinking

Starting at $210.00 per sample

Peptide conjugation to protein carriers (eg, KLH, BSA)

Peptide conjugation to protein carriers (eg, KLH, BSA)

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Monoclonal Antibody (mAb) Production

Starting at $4.00 per sample

Our custom monoclonal project process is designed to produce novel antibodies against protein, peptide, or other antigenic targets.

We work closely with each lab to maximize the success rate for obtaining antibodies that perform in the assays of interest to the investigator.

Our custom monoclonal project process is designed to produce novel antibodies against protein, peptide, or other antigenic targets.

We work closely with each lab to maximize the success rate for obtaining antibodies that perform in the assays of interest to the investigator.

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Biochemistry & Molecular Biology

Price on request
Request a quote for more information about this service.

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Biospecimens

Price on request
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Biology

Price on request
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Cell Lines

Price on request
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Chemistry and Materials

Price on request
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Cells and Tissues

Price on request
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Synthetic Chemistry

Price on request
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Protein Services

Price on request
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Protein Quantification

Price on request
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Biospecimen Management and Storage

Price on request
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Antibody Services

Price on request
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Rob Carnahan

Director

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