Cedjqwcntzgdewesgv0m final logo

Antibody Analytics

15 Orders Completed
Glasgow, GB

About Antibody Analytics

At Antibody Analytics we believe that scientists deserve the best data, and patients deserve the best drugs. Led by a group of talented and engaged scientists, we look at biological problems in an openminded way to deliver beautifully designed, developed and qualified bioassays. We have developed a... Show more »

At Antibody Analytics we believe that scientists deserve the best data, and patients deserve the best drugs. Led by a group of talented and engaged scientists, we look at biological problems in an openminded way to deliver beautifully designed, developed and qualified bioassays. We have developed a comprehensive portfolio of assays and platforms that can be tailored to support even the most complex of drug pipelines. Whether designing and developing bespoke bioassays or offering routine analysis on our already established assay platforms, Antibody Analytics has an exceptional track record of delivering beautiful results

Show less

Our Services (45)


ic

Surface Plasmon Resonance (SPR)

Price on request

Species specific screening

Using our in house capabilities (Biacore 8K) we deliver beautiful binding and kinetic data, that will let you understand your molecule better...

How are we going to do things differently?

• From our experience, customers can get frustrated by a lack of flexibility in data generation, the... Show more »

Species specific screening

Using our in house capabilities (Biacore 8K) we deliver beautiful binding and kinetic data, that will let you understand your molecule better...

How are we going to do things differently?

• From our experience, customers can get frustrated by a lack of flexibility in data generation, the resistance from service providers to move away from duplicate data. Antibody Analytic’s assays are set up to deliver triplicate data, which will allow a true percentage CV to be determined – an industry gold standard in our minds.

• An Fc capture approach will be utilised for most of our target binding assessments, as this is widely regarded as an industry standard and many of our large biotech customers favour this. Where Fc capture is not possible, we have plans and the ability to perform a method whereby the orientation of the setup is reversed (still a highly biologically relevant approach).

Show less
Biacore 8000 Immunology Oncology Immuno-Oncology Inflammation Autoimmune disease Biomarker discovery Cytokine analysis monoclonal comparison testing receptor ligand binding assays Biosimilarity analysis Biophysical Characterization kinetic assays Surface plasmon resonance (SPR) Kinetic analysis functional assays antibody screening antibody validation target validation bioanalysis Bioanalytical assay development bioanalytical assay validation Protein interaction analysis protein interaction binding Antibodies Human tissue Biotherapeutics Biopharmaceuticals/Biologics Monoclonal antibodies Biology Drug Discovery Biacore Large molecules/Biologics Small molecules Show 36 more tags Show less

ic

Mixed Lymphocyte Reactions

Price on request

The Mixed Lymphocyte Reaction (MLR) is a simple approach to emulate the interaction of antigen presenting cells (APCs) and T cells, which results to the activation and proliferation of the latter. Our MLRs provide a robust platform to determine the effects of biologics or other compounds on the ability of T cells to be activated... Show more »

The Mixed Lymphocyte Reaction (MLR) is a simple approach to emulate the interaction of antigen presenting cells (APCs) and T cells, which results to the activation and proliferation of the latter. Our MLRs provide a robust platform to determine the effects of biologics or other compounds on the ability of T cells to be activated and proliferate. Drug treatments that potentiate T cell proliferation and production of cytokines such as IFN-g in the MLR would indicate their potential ability to mount anti-tumour T cell responses in the tumour microenvironment. Inversely, compounds that inhibit T cell proliferation in the MLR could indicate their usefulness as treatments for autoimmune disease or for induction of transplantation tolerance.
Various sources of APCs and modes of their activation/maturation can be used in our MLR assay such as:
• Mixed PBMCs, our simplest but still very powerful format
• Monocytes (CD14+ cells purified from PBMC of healthy donors)
• Monocyte-derived dendritic cells (CD14+ cells purified from PBMC of healthy donors cultured for seven days in the presence of GM-CSF and IL-4)
• M1 or M2 macrophages (CD14+ cells purified from PBMC of healthy donors cultured for seven days in the presence of GM-CSF or M-CSF, respectively).
Our APCs can be matured using a variety of factors including LPS (and other TLR ligands) or cytokines (e.g. TNF).
We employ several readouts for out MLRs including T cell proliferation (CFSE, Click-iT EDU or Ki-67), high-content cytokine analysis and T cells and APC surface marker expression analysis (including co- inhibitory markers such as PD-1, LAG-3, Tim-3, CTLA-4 etc.).

Show less
Oncology Inflammation assay development Potency Assay FACS Cell-based assays Method Development Biosimilarity analysis functional assays Biopharmaceuticals/Biologics Monoclonal antibodies Drug Discovery Large molecules/Biologics Show 13 more tags Show less

ic

ADCC and CDC Assays

Price on request

ADCC (antibody-dependent cellular cytotoxicity) is an immune defence mechanism in which certain cells of the body are targeted for destruction by antibodies and subsequently killed by effector cells of the immune system. Many therapeutic antibodies and antibody-like proteins can harness and direct this killing activity towards... Show more »

ADCC (antibody-dependent cellular cytotoxicity) is an immune defence mechanism in which certain cells of the body are targeted for destruction by antibodies and subsequently killed by effector cells of the immune system. Many therapeutic antibodies and antibody-like proteins can harness and direct this killing activity towards cell types that express the antigen to which they're directed. Characterising the in vitro activity and establishing the potential influence on clinical efficacy poses one of the greatest challenges to biosimilar developers.

Antibody Analytics has developed a revolutionary approach and offers services based upon a proprietary advanced ADCC platform technology. Using novel cell lines and approaches we have eliminated the issues associated with traditional performance of ADCC assays and are able to provide elegant methods that measure target cell death with varying levels of biological relevance and sensitivity, all with unprecedented levels of performance and consistency.

Methodologies based upon the advanced ADCC assay platform are able to play a pivotal role in any biosimilar ADCC characerisation strategy adding significant value by providing data of the highest possible quality.

Expertise in ADCC Characterisation Strategies

No single approach can be applied to characterise the ADCC activity of a biosimilar and a successful ADCC characterisation strategy is dependent upon the (multiple) mechanism of actions of the innovator drug, its approved indications and most importantly, upon the observed activity of the biosimilar candidate itself relative to the reference innovator molecule. An ADCC evaluation strategy must therefore encompass a range of orthogonal analytical techniques that range from relatively simply CD16a (Fcγ Receptor IIIa) binding assessments to cell based ADCC assays. The complexity of ADCC assays can significantly increase as the nature of target and effector cell preparations are varied to meet specific objectives of the characterisation strategy.

Our experts have an unparalleled understanding of ADCC assay performance combined with industry leading expertise in the design and implementation of ADCC characterisation strategies. With knowledge gleaned from different biosimilar molecules that demonstrate a diverse range of similarity relative to the reference innovator product, we can help define a successful ADCC characterisation strategy for your biosimilar and perform the required experiments.

Our ADCC characterisation services include CD16a (Fcγ Receptor IIIa) binding assays, cell based binding assays (ELISA and Flow Cytometry) for measurement of antibody-antigen at the cell membrane and a range of methods based upon the advanced ADCC assay platform and a diverse range of investigatory ADCC assay formats that, together, are designed to truly understand the biological relevance and clinical significance of differences in ADCC activity. We can provide ADCC characterisation services for the following biosimilar molecules.

Adalimumab (Humira)
Bevacizumab (Avastin)
Denosumab (Prolia)
Etanercept (Enbrel)
Golimumab (Simponi)
Infliximab (Remicade)
Rituximab (Rituxan/MabThera)
Tocilizumab (Actemra)
Trastuzumab (Herceptin)
Ustekinumab (Stelara)

CDC Assays
The complement system plays a pivotal role in the innate immune system acting as an effector of adaptive immunity to remove common pathogens and unwanted cell types. The complement system can be activated through three major pathways: the classical, alternative and mannose-binding lectin. With relevance to therapeutic antibodies, the classical pathway is activated following binding of the C1 complex (C1q with C1r and C1s serine proteases) to the Fc region of a complement-fixing antibody that is bound to a target cell, initiating the complement cascade to kill cells through a process called complement-dependent cytotoxicity (CDC).

Determination of CDC activity of a biosimilars antibody should initially be conducted using a testing strategy composed of two orthogonal methods (a C1q binding assessment combined with a functional cell based CDC assay format) to determine the levels of similarity between the biosimilar and the reference innovator product. Where necessary, a range of investigatory assay formats can be applied to determine the significance of any differences on clinical efficacy and safety to demonstrate similarity.

Expertise in CDC Characterisation Strategies

Of the numerous effector function strategies that are employed to characterise biosimilars, a CDC assessment is probably the most simplistic as the number of required assay formats is reduced and those that are employed are less technically demanding than ADCC and ADCP. However, CDC activity of biosimimlar is still significantly influenced by certain product attributes that can vary between a biosimilar and the reference innovator product and it is critical that both C1q binding assays and functional cell based CDC assays demonstrate sufficient sensitive to these known product attributes and behave, together, as orthogonal methods.

Our experts are industry leaders in the understanding of the requirements for effector function characterisation having worked with a range of different biosimilar molecules that demonstrate a diverse range of similarity relative to the reference innovator product. With a wide portfolio of assays for different biosimilar molecules, we can help define a successful CDC characterisation strategy for your biosimilar and perform the required experiments.

Our CDC characterisation services include C1q binding assays, functional cell based methods using novel cell lines coupled with capabilities to conduct a diverse range of investigatory CDC assay formats for the following molecules:

Adalimumab (Humira)
Bevacizumab (Avastin)
Denosumab (Prolia)
Etanercept (Enbrel)
Golimumab (Simponi)
Infliximab (Remicade)
Rituximab (Rituxan/MabThera)
Tocilizumab (Actemra)
Trastuzumab (Herceptin)
Ustekinumab (Stelara)

Show less
Immunology Oncology Immuno-Oncology Inflammation Rheumatoid Arthritis Crohn's disease Autoimmune disease assay development Potency Assay Cell-based assays assay optimization bioassay Method Development Biosimilarity analysis In vitro bioassays functional assays Antibodies Biotherapeutics Biopharmaceuticals/Biologics Primary Cells Monoclonal antibodies Biology Drug Discovery HER2 VEGFR2 Large molecules/Biologics Show 26 more tags Show less

ic

In vitro Reporter Gene Assays

Price on request
Request a quote for more information about this service.

ic

T-Cell Exhaustion Assay

Price on request

Whether CAR-T, cytokines or checkpoint inhibitors, Antibody Analytics is equipped to support your development needs. We have a comprehensive portfolio of assays and services that can be tailored to suit your requirements. By taking our platform methods and tailoring them to suit, Antibody Analytics enables our customers to better understand the immunomodulatory effect of their drugs.

Whether CAR-T, cytokines or checkpoint inhibitors, Antibody Analytics is equipped to support your development needs. We have a comprehensive portfolio of assays and services that can be tailored to suit your requirements. By taking our platform methods and tailoring them to suit, Antibody Analytics enables our customers to better understand the immunomodulatory effect of their drugs.

Show less
Immunology Oncology Immuno-Oncology Flow cytometry assay development Flow bioassay sample validation Method Qualification Custom Assays Biology Drug Discovery Toxicology Show 13 more tags Show less

ic

Cytotoxic T Cell Assays

Price on request

The biological activity of T-cell engaging bispecific antibodies can be determined using TDCC (T-Cell Dependent Cellular Cytotoxicity) assays supported with CD3-antigen binding assays. The TDCC assay follows a similar format to that of the ADCC assay, in which the bispecific forms a bridge between target cells expressing and... Show more »

The biological activity of T-cell engaging bispecific antibodies can be determined using TDCC (T-Cell Dependent Cellular Cytotoxicity) assays supported with CD3-antigen binding assays. The TDCC assay follows a similar format to that of the ADCC assay, in which the bispecific forms a bridge between target cells expressing and T-cells present within the effector preparation. As with ADCC assays, TDCC assays are complex methods with many formats requiring the use of primary cell types and expertise to run successfully.

With proprietary cell lines expressing common bispecific antigen targets, cell line development capabilities and access to a wide variety of primary T cells, we can develop industry-leading TDCC assays to support early screening activities through to development of lot release potency methods.

Show less
Immunology Oncology Inflammation Flow cytometry assay development Potency Assay Cytotoxicity FACS Cell-based assays assay optimization bioassay Method Development Biosimilarity analysis In vitro bioassays functional assays Biopharmaceuticals/Biologics Monoclonal antibodies Drug Discovery Large molecules/Biologics Show 19 more tags Show less

ic

ADCP Assay

Antibody-Dependent Cell-Mediated Phagocytosis Assay
Price on request

With regulatory agencies requesting a primary cell ADCP format more regularly, Antibody Analytics has developed an assay format that, although technically challenging, directly measures the engulfment of the target cells by CD14+ effector cells.

  • Established network of over 75 genotyped donors
  • Using cryopreservation, we can... Show more »

With regulatory agencies requesting a primary cell ADCP format more regularly, Antibody Analytics has developed an assay format that, although technically challenging, directly measures the engulfment of the target cells by CD14+ effector cells.

  • Established network of over 75 genotyped donors
  • Using cryopreservation, we can analyse up to 100 ADCP samples from a single collection
  • Highly accurate, reliable and repeatable format
  • Allows for quantitative and/or qualitative data reporting
  • The most informative means of determining if structural differences between molecules that are likely to manifest as differences in clinical efficacy
Show less
Immunology Oncology Inflammation Flow cytometry assay development Potency Assay Cytotoxicity FACS assay optimization bioassay Method Development Biosimilarity analysis In vitro bioassays functional assays Biopharmaceuticals/Biologics Monoclonal antibodies Biology Drug Discovery Large molecules/Biologics Show 19 more tags Show less

ic

Biopharmaceutical Characterization

Price on request

Custom design, development and qualification of biosimilar assays to support biopharmaceutical development. Currently have assays to support the following molecules:

Adalimumab (Humira)
Bevacizumab (Avastin)
Denosumab (Prolia)
Etanercept (Enbrel)
Golimumab (Simponi)
Infliximab (Remicade)
Rituximab... Show more »

Custom design, development and qualification of biosimilar assays to support biopharmaceutical development. Currently have assays to support the following molecules:

Adalimumab (Humira)
Bevacizumab (Avastin)
Denosumab (Prolia)
Etanercept (Enbrel)
Golimumab (Simponi)
Infliximab (Remicade)
Rituximab (Rituxan/MabThera)
Tocilizumab (Actemra)
Trastuzumab (Herceptin)
Ustekinumab (Stelara)

If your molecule is not listed, please get int touch, as we are constantly developing to support the global biosimilar market

Show less
Flow cytometry assay development Potency Assay FACS Cell-based assays assay optimization bioassay Method Development ligand binding assays Biosimilarity analysis In vitro bioassays functional assays Show 12 more tags Show less

ic

Cell Viability & Proliferation Assays

Price on request

Custom design, development and qualification of cell viability and proliferation assays to support biopharmaceutical development.

Custom design, development and qualification of cell viability and proliferation assays to support biopharmaceutical development.

Show less
Flow cytometry assay development Potency Assay Cytotoxicity FACS Cell-based assays assay optimization bioassay proliferation Method Development Biosimilarity analysis In vitro bioassays functional assays Cell Proliferation Assays Show 14 more tags Show less

ic

Apoptosis Assays

Price on request

Custom design, development and qualification of cell death and apoptosis assays to support biopharmaceutical development.

Custom design, development and qualification of cell death and apoptosis assays to support biopharmaceutical development.

Show less
in vitro toxicity assay development Potency Assay Cytotoxicity Cell-based assays assay optimization bioassay Method Development Biosimilarity analysis In vitro bioassays functional assays Show 11 more tags Show less

ic

Biopharmaceutical Development

Price on request

The Antibody Analytics management team have been providing biosimilar characterisation services for over 5 years working across a wide variety of assay formats and molecules and offer characterisation services based upon the following biosimilar molecules/methods:

Adalimumab (Humira): TNF-alpha binding and cell based TNF-alpha... Show more »

The Antibody Analytics management team have been providing biosimilar characterisation services for over 5 years working across a wide variety of assay formats and molecules and offer characterisation services based upon the following biosimilar molecules/methods:

Adalimumab (Humira): TNF-alpha binding and cell based TNF-alpha neutralisation bioassays
Bevacizumab (Avastin): VEGF binding and cell based VEGF neutralisation bioassays
Denosumab (Prolia): RANKL binding and cell based RANKL neutralisation bioassays
Etanercept (Enbrel): TNF-alpha binding and cell based TNF-alpha neutralisation bioassays
Golimumab (Simponi): TNF-alpha binding and cell based TNF-alpha neutralisation bioassays
Infliximab (Remicade): TNF-alpha binding and cell based TNF-alpha neutralisation bioassays
Tocilizumab (Actemra): IL-6R binding and cell based IL-6 neutralisation bioassays
Ustekinumab (Stelara): IL-12 binding and cell based IL-12 neutralisation bioassay

Show less
Flow cytometry assay development Potency Assay Cytotoxicity FACS Cell-based assays Cytokine analysis assay optimization bioassay Method Development ligand binding assays Biosimilarity analysis In vitro bioassays functional assays Show 14 more tags Show less

ic

Real-Time Cytotoxicity Assay

Price on request
Request a quote for more information about this service.
XCELLigence

ic

Immuno-oncology Assays

Price on request

Hailed as one of the most exciting and innovative approaches to fighting cancer, Immuno-oncology is a means of using the body's own immune system to overcome disease. Whether CAR-T, cytokines or checkpoint inhibitors, Antibody Analytics is equipped to support your development needs. We have a comprehensive portfolio of assays and... Show more »

Hailed as one of the most exciting and innovative approaches to fighting cancer, Immuno-oncology is a means of using the body's own immune system to overcome disease. Whether CAR-T, cytokines or checkpoint inhibitors, Antibody Analytics is equipped to support your development needs. We have a comprehensive portfolio of assays and services that can be tailored to suit your requirements.

Our services include a diverse range of assay formats:

Exhausted CD4+T cell assays
Assessment of Activation of Innate cells
Human Mixed Lymphocyte Reaction (MLR)
Human DC, Macrophage and Co-culture assays
T-cell killing assays
ADCC, ADCP, Trogocytosis
Antigen binding

http://www.antibodyanalytics.com/immuno-oncology

Show less
Immunology Oncology Immuno-Oncology Inflammation Breast cancer Flow cytometry Cell-based assays Lymphocyte Proliferation Assay monoclonal bioassay ligand binding assays Monocyte activation testing functional assays Cell Proliferation Assays Biology Drug Discovery antibody PD-L1 bioassay Show 19 more tags Show less

ic

Cell-Based Assays

Price on request

Binding and Cell Based Potency Assays

The potency of a therapeutic biologic is most commonly determined using a cell based bioassay and is frequently supported by a complimentary binding assay that is based upon the primary mechanism of action (MOA) of the therapeutic. Such methods play a critical role at all stages of... Show more »

Binding and Cell Based Potency Assays

The potency of a therapeutic biologic is most commonly determined using a cell based bioassay and is frequently supported by a complimentary binding assay that is based upon the primary mechanism of action (MOA) of the therapeutic. Such methods play a critical role at all stages of product development and are essential tools that are employed during clone selection, process development and optimisation and will ultimately feature as the most critical of the lot release assays.

The importance of getting the method correct cannot be overstated and considerably attention should be applied during development to ensuring that robust methods are built in line with best practices, provide consistent, accurate and precise results over time and are stability indicating. With a comprehensive knowledge of the bioassay requirements across clinical phases and therapeutics classes, Antibody Analytics offers a range of best-in-class binding and cell based assays to characterise biosimilars using QuBas Bioassay Software and can apply our expertise to develop high quality potency methods for novel therapeutics.

Show less

ic

Cell Signaling Assays

Price on request
Request a quote for more information about this service.

ic

T Cell Activation and Proliferation Assays

Price on request

Antibody Analytics’ has extensive expertise in assessing the impact of therapeutics on T cell activation. Isolated T cells or PBMCs can be activated with antibodies (CD3/CD28) in the presence of small molecules or biologics and activation is measured in the form of cell surface markers, cytokine release and cell proliferation.... Show more »

Antibody Analytics’ has extensive expertise in assessing the impact of therapeutics on T cell activation. Isolated T cells or PBMCs can be activated with antibodies (CD3/CD28) in the presence of small molecules or biologics and activation is measured in the form of cell surface markers, cytokine release and cell proliferation. Furthermore, we are in the process of developing artificial antigen presenting cells, in the form of beads that carry on their surface antibodies (e.g. anti-CD3/anti-CD28) and recombinant proteins (e.g. PD-L1) that can be utilised for assessment of biologics that target co-stimulatory and co-inhibitory pathways.

Antibody Analytics has access to PBMCs and T cells from multiple donors that addresses donor to donor variability. We can provide several assay readouts including high content data such as multiplex cytokine analysis and multiparameter flow cytometry. Furthermore, we can provide analysis and interpretation of the effects of your molecule to allow you to make meaningful decisions for your development program.

http://www.antibodyanalytics.com/immuno-oncology/t-cell-activation-assays

Show less

ic

Cell-Based Screening Methods

Price on request

Understanding the exact mechanisms of a disease is vitally important. In order to better comprehend the pathology of the disease, many developers and researchers rely on in vitro methods which can often provide more insight than target-based screening alone. Phenotypic screening allows researchers to identify new chemical entities... Show more »

Understanding the exact mechanisms of a disease is vitally important. In order to better comprehend the pathology of the disease, many developers and researchers rely on in vitro methods which can often provide more insight than target-based screening alone. Phenotypic screening allows researchers to identify new chemical entities and biomarkers without prior knowledge, or bias, of the target(s) underlying the response.

Show less

ic

Functional T-cell Suppression Assays

Price on request

Immune checkpoint molecules such as PD-L1 on their surface to inhibit T cell activity, the generalized immune-inhibitory tumor microenvironment can also polarize T cells to a suppressive phenotype known as regulatory T cells, or Tregs. Tregs are CD4+ T cells characterized by the secretion of the regulatory cytokine IL-10, which is... Show more »

Immune checkpoint molecules such as PD-L1 on their surface to inhibit T cell activity, the generalized immune-inhibitory tumor microenvironment can also polarize T cells to a suppressive phenotype known as regulatory T cells, or Tregs. Tregs are CD4+ T cells characterized by the secretion of the regulatory cytokine IL-10, which is inhibitory to both macrophages and T cells, and can to suppress the activity of tumor-directed T cells.

Custom design, development and qualification of cytotoxicity assays to support biopharmaceutical developent

Show less

ic

Cell Death Assays

Price on request

Antibody Analytics provides reliable, robust and accurate analysis of effector function activity of a range of mAb and mAb-like drug candidates
Using innovative approaches to develop custom assays for novel therapeutics e.g. cell therapy, bi-specifics and immunology, together with beautifully pre-developed bioassays for high... Show more »

Antibody Analytics provides reliable, robust and accurate analysis of effector function activity of a range of mAb and mAb-like drug candidates
Using innovative approaches to develop custom assays for novel therapeutics e.g. cell therapy, bi-specifics and immunology, together with beautifully pre-developed bioassays for high throughput biosimilar candidate analysis, you can rest assured that we are the leaders in biological analysis.

Our revolutionary approach based upon proprietary platform technologies, using novel cell lines and methodologies, eliminates issues associated with traditional performance of effector function assays with unparalleled levels of performance and consistency.

Some of the assays we can support:

PBMC and NK based ADCC
Whole blood cytotoxicity
CDC
TDCC
Trogocytosis

Show less

ic

Specialized Cell-Based Assays

Price on request

Antibody Analytics have developed an extensive range of internal assay formats to characterise biologics to the highest standard and industry leading data analysis using QuBas Bioassay Software. Our platform methodologies can be readily adapted to characterise novel products delivering high quality methods whilst reducing cost and... Show more »

Antibody Analytics have developed an extensive range of internal assay formats to characterise biologics to the highest standard and industry leading data analysis using QuBas Bioassay Software. Our platform methodologies can be readily adapted to characterise novel products delivering high quality methods whilst reducing cost and timelines. Whether you are early in development and looking to elucidate a mechanism of action or at a later phase and wish to establish an improved lot release potency methodology, Antibody Analytics has the expertise to help you.

Our services include a diverse range of assay formats

Cell-based Binding Assays (Flow Cytometry & ELISA)
Cell Death & Apoptosis
Cell Proliferation & Viability
Cell Signalling
Cytokine Secretion/Inhibition
Effector Function Assays (ADCC, CDC, ADCP & TDCC)
Ligand Binding assays
Reporter Gene Assays

Show less

ic

Functional & Cell Type Specific Assays

Price on request

Antibody Analytics have developed an extensive range of internal assay formats to characterise biologics to the highest standard and industry leading data analysis using QuBas Bioassay Software. Our platform methodologies can be readily adapted to characterise novel products delivering high quality methods whilst reducing cost and... Show more »

Antibody Analytics have developed an extensive range of internal assay formats to characterise biologics to the highest standard and industry leading data analysis using QuBas Bioassay Software. Our platform methodologies can be readily adapted to characterise novel products delivering high quality methods whilst reducing cost and timelines. Whether you are early in development and looking to elucidate a mechanism of action or at a later phase and wish to establish an improved lot release potency methodology, Antibody Analytics has the expertise to help you.

Our services include a diverse range of assay formats

Cell-based Binding Assays (Flow Cytometry & ELISA)
Cell Death & Apoptosis
Cell Proliferation & Viability
Cell Signalling
Cytokine Secretion/Inhibition
Effector Function Assays (ADCC, CDC, ADCP & TDCC)
Ligand Binding assays
Reporter Gene Assays

Show less

ic

Cytotoxicity Assays

Price on request

With a comprehensive understanding of the MOAs of the biopharmaceutical coupled with world class approaches for in vitro characterisation, we are well placed to both assist you in defining appropriate investigatory approaches and perform the necessary testing. Our internal developmental focus is a range of investigatory bioassays... Show more »

With a comprehensive understanding of the MOAs of the biopharmaceutical coupled with world class approaches for in vitro characterisation, we are well placed to both assist you in defining appropriate investigatory approaches and perform the necessary testing. Our internal developmental focus is a range of investigatory bioassays that focus upon secondary and tertiary activities of these molecules and we are happy to discuss other molecules.

Show less

ic

Drug Discovery & Development

Price on request

Antibody Analytics can support drug developers from drug discovery, target identification and validation and can develop binding and functional analysis to support throughout your drug development.

Antibody Analytics can support drug developers from drug discovery, target identification and validation and can develop binding and functional analysis to support throughout your drug development.

Show less

ic

CD20 Assays

Price on request

Rituxan/Mabthera (rituximab) a chimeric human/murine monoclonal antibody targeted against the CD20 antigen expressed on the cell surface of B cells. Therapeutically used in the treatment of haematological cancers (leukaemias and lymphomas) and autoimmune dysfunction (rheumatoid arthritis), Rituximab is described on the list of... Show more »

Rituxan/Mabthera (rituximab) a chimeric human/murine monoclonal antibody targeted against the CD20 antigen expressed on the cell surface of B cells. Therapeutically used in the treatment of haematological cancers (leukaemias and lymphomas) and autoimmune dysfunction (rheumatoid arthritis), Rituximab is described on the list of Essential Medicines important for a basic health system by the World Health Organisation.

oth the exact function of CD20 and the exact mechanisms of action of Rituxan (Rituximab) are not fully understood, however both Complement Dependent Cytotoxicity (CDC) and Antibody Dependent Cellular Cytoxicity (ADCC) are important mechanisms of action. The Fab-related functions of Rituxan (Rituximab) also cause inhibition of proliferation and induction of apoptosis when applied as a homodimer to CD20+ cell lines.

Show less

ic

Cytokine Release Assay

Price on request

Investigatory bioassays provide solutions to sound hypotheses associated with a monoclonal antibody-based drug molecule. These exploratory assays aim to provide a deeper understanding of potential in vivo activities.

Investigatory bioassays provide solutions to sound hypotheses associated with a monoclonal antibody-based drug molecule. These exploratory assays aim to provide a deeper understanding of potential in vivo activities.

Show less

ic

Inflammation Assays

Price on request

Investigatory bioassays provide solutions to sound hypotheses associated with a monoclonal antibody-based drug molecule. These exploratory assays aim to provide a deeper understanding of potential in vivo activities.

Investigatory bioassays provide solutions to sound hypotheses associated with a monoclonal antibody-based drug molecule. These exploratory assays aim to provide a deeper understanding of potential in vivo activities.

Show less

ic

Immune Cell Assays

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

T Cell Inhibition Assays

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

Custom Immune Cell Assays

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

B Cell Activation Assay

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

Bioanalytical Assay Development and Optimization

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

Bioanalytical Assay Validation

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

Phagocytosis Assay

Price on request

ADCP, which is commonly mediated by macrophages, neutrophils and mature dendritic cells, is a significant mechanism of action of therapeutic antibodies with signals transduced to the effector cell via Fc receptors. The role of human Fc receptors, FcγRI, FcRγRII and FcRγRIII, in phagocytosis is well established and complete... Show more »

ADCP, which is commonly mediated by macrophages, neutrophils and mature dendritic cells, is a significant mechanism of action of therapeutic antibodies with signals transduced to the effector cell via Fc receptors. The role of human Fc receptors, FcγRI, FcRγRII and FcRγRIII, in phagocytosis is well established and complete inhibition of phagocytosis in the presence of blocking antibodies, which prevent Fc receptor interactions with target bound IgG, has been demonstrated. A potentially potent mechanism of antibody therapy in cancer treatment is macrophage mediated phagocytosis, which involves the ingestion of opsonized target by macrophages; macrophages are known to be in abundance in tumour stroma.

Show less

ic

Natural Killer Cell Cytotoxicity Assay

Price on request

NK enriched ADCC bioassays

During the development of an in vitro biological assay to characterise the functional activity of a monoclonal antibody therapeutic several considerations should be taken into account:

Sensitivity of the assay to detect small changes in the biological function
Robustness of the method to day-to-day variability
Biological relevance of the approach

NK enriched ADCC bioassays

During the development of an in vitro biological assay to characterise the functional activity of a monoclonal antibody therapeutic several considerations should be taken into account:

Sensitivity of the assay to detect small changes in the biological function
Robustness of the method to day-to-day variability
Biological relevance of the approach

Show less

ic

Cell Invasion & Migration Assays

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

Bioanalytical Assays

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

Bioanalysis

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

Custom Immunoassay Development

Price on request

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Antibody Analytics designs and develops beautiful bioassays to support biosimilar and NBE development, including immuno-oncology, cell therapy and ADCs. Using industry leading know-how and state of the art processes, we have accelerated development for some of the leading biotech companies in the world.

Find out where our bioanalytical testing services can support you!

Show less

ic

PD-1 Assays

Programmed Cell Death Protein-1 Checkpoint Assays
Price on request

Cell-based immune-checkpoint assays
PD-1, PD-L1, CTLA-4, OX-40, CD-40, GITR, 4-1BB, TIGIT, LAG3

Cell-based immune-checkpoint assays
PD-1, PD-L1, CTLA-4, OX-40, CD-40, GITR, 4-1BB, TIGIT, LAG3

Show less

ic

Cell Signaling Analyses

Price on request

Cell Signaling Analyses Services

Cell Signaling Analyses Services

Show less

ic

Assay Development

Price on request
Request a quote for more information about this service.

ic

Biomolecular Interaction Analysis

Price on request

Biomolecular Interaction Analysis Services

Biomolecular Interaction Analysis Services

Show less

ic

Biochemistry & Molecular Biology

Price on request

Biochemistry & Molecular Biology Services

Biochemistry & Molecular Biology Services

Show less

ic

Pharmacology

Price on request

Pharmacology Services

Pharmacology Services

Show less

ic

Ligand Binding Assay (LBA) Bioanalysis

Price on request
Request a quote for more information about this service.

Not finding what you're looking for?

Get info on this provider's capabilities without requesting a quote.
Missing Avatar

Andrew Baron

Director of Operations
Missing Avatar

Terry Gray

Director - Strategic Projects
Missing Avatar

Jamie Hodgson

Director - Strategic Partnerships
Missing Avatar

Stuart Knowling

Technical Director - Biophysics
Missing Avatar

Justyna Rzepecka

Associate Director
Missing Avatar

Andrew Upsall

Managing Director
Missing Avatar

Nicola Woods

Scientist

FileType/PDF Created with Sketch. Translational Immunology.pdf

Click to Download
  • Positive review for ADCP Assay:

    April 2018

    "Antibody Analytics offers high-quality, custom assay development and testing services. It's hard to find experienced companies that will perform method development, particularly for specialized assays using primary cells, and they exceed expectations in their project engagement, trouble-shooting and results delivery. I highly recommend them for both custom and off the shelf assay needs."

Antibody Analytics has not received any endorsements.