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Animal Models Shared Resource (AMSR) - Zebrafish

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Washington, District of Columbia, US

About Animal Models Shared Resource (AMSR) - Zebrafish

We provide an extensive range of services to enable investigators to utilize this powerful animal model. Zebrafish core personnel offer consultation for experimental design and analysis, as well as training at all levels in the use of zebrafish as a model system. The zebrafish core houses wild-type, mutant and... Show more »

We provide an extensive range of services to enable investigators to utilize this powerful animal model. Zebrafish core personnel offer consultation for experimental design and analysis, as well as training at all levels in the use of zebrafish as a model system. The zebrafish core houses wild-type, mutant and transgenic fish lines, and provides live embryos and larvae for toxicity/small compound screening, behavioral testing, xenografting and teaching purposes. Staged embryos and larvae, fixed for in situ hybridization and immunohistochemical analyses, as well as DNA, RNA or proteins are available. A wide range of specialized services are provided by the (AMSR) including: Morpholino oligonucleotide (MO) injection to knock down specific genes of interest; RNA or cDNA injection to over-express wild-type, mutant and chimeric genes; generation of transgenic zebrafish; generation of mutant fish by targeted CRISPR-Cas9 gene knockout; cell transplantation; xenograft assays; in situ hybridization, immunohistochemistry, and histochemistry; imaging; and teratogenicity and toxicity assays for small molecule screening.

Please note: As a not-for-profit institution (Georgetown University) we follow the CRISPR genome editing guidelines as dictated by the Broad Institute, MIT, and Harvard to made CRISPR technology broadly available to the research community. Our zebrafish work requires only a single plasmid containing the Cas9 gene as provided by Addgene, a non-profit plasmid repository that distributes the plasmid through the generosity of the Zhang lab. It is only our intentions to provide CRISPR genome editing services to other academic and not-for-profit institutions.

Relevant publications:

  • Caitrín M. Coffey, Patricia A. Solleveld, Joyce Fang, Antonia K. Roberts, Sung-Kook Hong, Igor B. Dawid, Caroline E. Laverriere and Eric Glasgow. Novel Oxytocin Gene Expression in the Hindbrain is Induced by Alcohol Exposure: Transgenic Zebrafish Enable Visualization of Sensitive Neurons. PLoSOne 2013;8(1):e53991. doi: 10.1371/journal.pone.0053991. Epub 2013 Jan 14. PMID: 23342055 PMCID: PMC3544674

  • Olga Catalina-Rodriguez, Vamsi K. Kolukula, York Tomita, Anju Preet, Ferdinando Palmieri, Anton Wellstein, Stephen Byers, Amato J. Giaccia, Eric Glasgow and Maria Laura Avantaggiati. The mitochondrial citrate transporter, CIC, is essential for mitochondrial homeostasis. Oncotarget. 2012 Oct;3(10):1220-35. PMID: 23100451

  • Adhikari, S., Karmahapatra, S.K., Karve, T.M., Bandopadhya, S., Woodrick, J., Manthena, P.V., Glasgow, E., Byers, S., Saha, T., Uren, A. Characterization of magnesium requirement of human 5’-tyrosyl DNA phosphodiesterase mediated reaction. BMC Res Notes. 2012 Mar9;5:134. PMID: 22405347 PMCID: PMC3315744

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Our Services (23)


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In vivo Teratogenicity Testing

Price on request

Environmental samples or small molecules are screened using 96-well format for toxicity (by scoring for death) or teratogenicity based on altered morphology. In addition, transgenic reporter lines can be screened for effects on specific molecules or organ systems. Phenotypes are scored and documented by photomicroscopy.
Toxicity: $200 per 96 well plate
Teratogenicity: $300 per 96 well plate

Environmental samples or small molecules are screened using 96-well format for toxicity (by scoring for death) or teratogenicity based on altered morphology. In addition, transgenic reporter lines can be screened for effects on specific molecules or organ systems. Phenotypes are scored and documented by photomicroscopy.
Toxicity: $200 per 96 well plate
Teratogenicity: $300 per 96 well plate

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Zebrafish

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Zebrafish Genome Editing

Starting at $300.00 per unit

Generation of mutant zebrafish lines by targeted gene editing
The CRISPR-Cas9 system is used for generation of targeted genome modification in zebrafish. Single target Cas9 endonuclease or paired target Cas9 nickase strategies are both utilized to generate knockout and transgenic animals. 20-mer targets are designed on-line and... Show more »

Generation of mutant zebrafish lines by targeted gene editing
The CRISPR-Cas9 system is used for generation of targeted genome modification in zebrafish. Single target Cas9 endonuclease or paired target Cas9 nickase strategies are both utilized to generate knockout and transgenic animals. 20-mer targets are designed on-line and confirmed to be unique in the zebrafish genome. Injection ready T7-generated zebrafish codon-optimized cas9 mRNA and guide RNA are produced in vitro and microinjected in single-cell zebrafish embryos. Injected embryos are screened for successful gene targeting and larvae are raised and bred until the confirmation of a successful heritable genome modification. $300/gRNA (injection ready), confirmed mutant generation price upon request.

Please note: As a not-for-profit institution (Georgetown University) we follow the CRISPR genome editing guidelines as dictated by the Broad Institute, MIT, and Harvard to made CRISPR technology broadly available to the research community. Our zebrafish work requires only a single plasmid containing the Cas9 gene as provided by Addgene, a non-profit plasmid repository that distributes the plasmid through the generosity of the Zhang lab. It is only our intentions to provide CRISPR genome editing services to other academic and not-for-profit institutions.

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Whole Mount in situ Hybridization

Starting at $575.00 per unit

Whole-mount in situ hybridization
Whole-mount in situ hybridization of zebrafish embryos. Colorimetric or fluorescent probes can be synthesized. Please contact us to discuss experimental details.
$575. (per probe)

Whole-mount in situ hybridization
Whole-mount in situ hybridization of zebrafish embryos. Colorimetric or fluorescent probes can be synthesized. Please contact us to discuss experimental details.
$575. (per probe)

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Zebrafish Models

Starting at $80.00 per unit

Staged embryos and larvae, fixed for in situ hybridization and immunohistochemical analyses.
$80. (per clutch of 50)

Staged embryos and larvae, fixed for in situ hybridization and immunohistochemical analyses.
$80. (per clutch of 50)

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Genomic DNA Extraction

Starting at $55.00 per unit

Zebrafish genomic DNA
Genomic DNA isolated from an individual fish or a clutch of 50 embryos

Zebrafish genomic DNA
Genomic DNA isolated from an individual fish or a clutch of 50 embryos

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Immunohistochemistry (IHC)

Starting at $575.00 per unit

Whole-mount immunohistochemistry
Whole-mount in immunohistochemistry of zebrafish embryos. Colorimetric or fluorescent detection is available. Please contact us to discuss experimental details.
$575. (per antibody)

Whole-mount immunohistochemistry
Whole-mount in immunohistochemistry of zebrafish embryos. Colorimetric or fluorescent detection is available. Please contact us to discuss experimental details.
$575. (per antibody)

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In situ Hybridization (ISH)

In Situ Hybridization
Starting at $575.00 per unit

Whole-mount in situ hybridization
Whole-mount in situ hybridization of zebrafish embryos. Colorimetric or fluorescent probes can be synthesized. Please contact us to discuss experimental details.
$575. (per probe)

Whole-mount in situ hybridization
Whole-mount in situ hybridization of zebrafish embryos. Colorimetric or fluorescent probes can be synthesized. Please contact us to discuss experimental details.
$575. (per probe)

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RNA Extraction and Purification

Starting at $105.00 per unit

Zebrafish RNA
RNA extracted from staged zebrafish embryos
$105. (isolated from clutch of 50)

Zebrafish RNA
RNA extracted from staged zebrafish embryos
$105. (isolated from clutch of 50)

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RNA Injection of Zebrafish Embryos

Starting at $337.50 per unit

Zebrafish eggs are microinjected with RNA in order to over-express wild-type, mutant or chimeric genes. RNA injection is a standard technique for ubiquitously expressing a gene of interest in zebrafish. The level of over-expression (dose) can be controlled by adjusting the amount of injected RNA. Please contact us to discuss experimental details.
$337.5 (clutch of 50 injected embryos)

Zebrafish eggs are microinjected with RNA in order to over-express wild-type, mutant or chimeric genes. RNA injection is a standard technique for ubiquitously expressing a gene of interest in zebrafish. The level of over-expression (dose) can be controlled by adjusting the amount of injected RNA. Please contact us to discuss experimental details.
$337.5 (clutch of 50 injected embryos)

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Zebrafish Xenograft

Price on request

Zebrafish xenograft assays

Human tumor cell xenograft into zebrafish makes use of the immature immune system and transparency of embryos to enable direct observation of tumor cell behavior in live animals. Tumor cell invasion, migration and metastases can be measured with this assay making it an attractive platform for testing... Show more »

Zebrafish xenograft assays

Human tumor cell xenograft into zebrafish makes use of the immature immune system and transparency of embryos to enable direct observation of tumor cell behavior in live animals. Tumor cell invasion, migration and metastases can be measured with this assay making it an attractive platform for testing the effects of gene manipulation or drug treatment on cancer cell behavior. Please contact us for more details.

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In vivo Toxicity Testing

Starting at $200.00 per unit

Environmental samples or small molecules are screened using 96-well format for toxicity (by scoring for death) or teratogenicity based on altered morphology. In addition, transgenic reporter lines can be screened for effects on specific molecules or organ systems. Phenotypes are scored and documented by photomicroscopy.
Toxicity: $200 per 96 well plate
Teratogenicity: $300 per 96 well plate

Environmental samples or small molecules are screened using 96-well format for toxicity (by scoring for death) or teratogenicity based on altered morphology. In addition, transgenic reporter lines can be screened for effects on specific molecules or organ systems. Phenotypes are scored and documented by photomicroscopy.
Toxicity: $200 per 96 well plate
Teratogenicity: $300 per 96 well plate

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Zebrafish

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Zebrafish Morpholino Injection

Price on request

Morpholino oligonucleotide (MO) injection is a standard technique for eliminating gene expression in zebrafish embryos. This technology provides a way of rapidly determining gene function in a vertebrate organism and can be accomplished in a matter of weeks. The AMSR can assist with MO experimental design, inject embryos, and... Show more »

Morpholino oligonucleotide (MO) injection is a standard technique for eliminating gene expression in zebrafish embryos. This technology provides a way of rapidly determining gene function in a vertebrate organism and can be accomplished in a matter of weeks. The AMSR can assist with MO experimental design, inject embryos, and analyze resulting phenotypes. Please contact us to discuss experimental details.

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Zebrafish Proteins

Starting at $125.00 per unit

Protein extracted from staged zebrafish embryos
$125. (isolated from clutch of 50)

Protein extracted from staged zebrafish embryos
$125. (isolated from clutch of 50)

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Zebrafish Research Consultation

Starting at $119.00 per hour

Consulting on all aspects of zebrafish research is provided, including the design of custom experimental protocols to fit specific requirements of individual investigators.
$119. /hr

Consulting on all aspects of zebrafish research is provided, including the design of custom experimental protocols to fit specific requirements of individual investigators.
$119. /hr

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Transgenic Zebrafish Development

Starting at $337.50 per unit

Zebrafish eggs are microinjected with DNA constructs in order to generate transgenic fish. Please contact us to discuss experimental details.
$337.5 (clutch of 50 injected embryos)

The injected fish are raised to adulthood and founders are identified by the presence of a fluorescent marker or genotyping progeny. $660. (clutch... Show more »

Zebrafish eggs are microinjected with DNA constructs in order to generate transgenic fish. Please contact us to discuss experimental details.
$337.5 (clutch of 50 injected embryos)

The injected fish are raised to adulthood and founders are identified by the presence of a fluorescent marker or genotyping progeny. $660. (clutch of 50 injected embryos)
$997.5 for identified transgenic fish (minimum 2 lines)

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Oncology Animal Models

Price on request
Request a quote for more information about this service.

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Fish, Reptile, and Amphibian Models

Price on request
Request a quote for more information about this service.

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Protein Expression Visualization

Price on request
Request a quote for more information about this service.

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Transgenic Animal Model Development

Price on request
Request a quote for more information about this service.

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Zebrafish Transgenic Services

Price on request
Request a quote for more information about this service.

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Xenograft Models

Price on request
Request a quote for more information about this service.

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Animal Models of Disease

Price on request
Request a quote for more information about this service.

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Drug Discovery & Development

Price on request

Drug Discovery & Development Services

Drug Discovery & Development Services

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Eric Glasgow

Director
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Matthew Swift

Manager
  • Positive review for Zebrafish Genome Editing:

    February 2017

    "I would like to work with Animal Models Shared Resource (AMSR) through Science Exchange again. They did the best on research, serve us, and answering all of our questions. Thank you, Dr. Eric and Ms. Lauren. Wendy Prabowo Aquatic Genomics and Breeding Lab"

  • Positive review for Zebrafish Genome Editing:

    December 2016

    "Always with clear explanations and helpful. . I truly recommend the service. ."

  • Positive review for Zebrafish Morpholino Injection:

    November 2016

    "very good standard of work."

  • Positive review for Zebrafish Morpholino Injection:

    May 2016

    "excellent service and very reasonably priced. An extremely valuable collaborator."

  • Positive review for Transgenic Zebrafish Development:

    November 2015

    "We are very satisfied with the work done for us and are planning on using them for another project."

  • Positive review for Zebrafish Morpholino Injection:

    January 2015

    "Outstanding service - provided key scientific insights, great flexibility and incredibly quick results."

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    Brian WalcottOctober 2016

    100% positive experience with this lab. Their expertise in the zebrafish model is obvious and the experiments were conducted timely and as described. Images/deliverables were of the highest quality. Hope to work with again in the future.

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    Adrian ListonDecember 2014

    I found this laboratory through Science Exchange and the commissioned project has been carried out extremely fast and to an outstanding scientific level. I would highly recommend this service provider to other researchers.

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