1352856160859 a2336ed1c2cc707a7d464a3906810725

Animal Models Core

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Chapel Hill, North Carolina, US

About Animal Models Core

The UNC Animal Models Core Facility provides the full range of mouse genetic modification services, reagents and related technologies. The Core's services are available to both UNC and Non-UNC clients.

Transgenic (pronuclear injection) animals can be generated in C57BL/6, FVB or other strains. Services... Show more »

The UNC Animal Models Core Facility provides the full range of mouse genetic modification services, reagents and related technologies. The Core's services are available to both UNC and Non-UNC clients.

Transgenic (pronuclear injection) animals can be generated in C57BL/6, FVB or other strains. Services for creating transgenic mice include transgene design, construct generation, isolation of the transgene fragment, pronuclear microinjection, founder genotyping and breeding.

The core also offers the full suite of services for BAC (bacterial artificial chromosome) recombineering and microinjection of BAC transgenes.

Gene targeting services are available in ES cells from C57BL/6, 129P2/Ola or BALB/c strains. Other strains available upon request. Gene targeting services include design and cloning of targeting vectors, creating gene-targeted ES cells, blastocyst microinjection of the ES cells to create transmitting chimeras and chimera breeding for germline transmission.

New in 2013: we now offer genome modification services using CRISPR/Cas nucleases. We can produce and validated the nucleases, then use them to generate knockout or knock-in mice, rats or cell lines.

The core can also provide Southern blot validation and blastocyst microinjection of ES cells from EUCOMM, KOMP or other consortia.

Additional services include Cre- or Flp-mediated excision in ES cells or in vivo, chromosome counting, PCR screening and Southern blotting.

Rat transgenic or knockou/knock-in model production is also available.

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Our Services (33)


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Recombineering

Recombination-Mediated Genetic Engineering
Price on request
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Transgenic Animal Model Development

Price on request

CRISPR/Cas nucleases can be designed to modify the genome, facilitating production of knockout or knock-in mice, rats or cell lines. Mouse and rat models can be produced in as little as 4 months! Contact us for a custom project quote.

CRISPR/Cas nucleases can be designed to modify the genome, facilitating production of knockout or knock-in mice, rats or cell lines. Mouse and rat models can be produced in as little as 4 months! Contact us for a custom project quote.

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Knockout Mouse Development

Price on request

We offer a full range of services for production of knockout, knockin or conditional knockout/knockin animals.

Knockout, conditional knockout and knockin mice can be custom designed to meet your project goals. Our full-service model production package includes design, targeting vector construction, ES cell electroporation, ES... Show more »

We offer a full range of services for production of knockout, knockin or conditional knockout/knockin animals.

Knockout, conditional knockout and knockin mice can be custom designed to meet your project goals. Our full-service model production package includes design, targeting vector construction, ES cell electroporation, ES cell screening, Southern blot validation, blastocyst injection and chimera breeding. Our service offerings are flexible to perform any parts of the process to meet individual client needs.

Knockout or knockin mice and rats can also be produced by microinjection of CRISPR/Cas engineered nucleases. We offer nuclease production and validation services in addition to microinjection. Contact us for details and pricing.

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Genetically Engineered Mice

Price on request

We offer a full range of services for production of knockout, knockin, transgenic and BAC transgenic animals.

Knockout, conditional knockout and knockin mice can be custom designed to meet your project goals. Our full-service model production package includes design, targeting vector construction, ES cell electroporation, ES... Show more »

We offer a full range of services for production of knockout, knockin, transgenic and BAC transgenic animals.

Knockout, conditional knockout and knockin mice can be custom designed to meet your project goals. Our full-service model production package includes design, targeting vector construction, ES cell electroporation, ES cell screening, Southern blot validation, blastocyst injection and chimera breeding. Our service offerings are flexible to perform any parts of the process to meet individual client needs.

Pronuclear microinjection services are available for production of transgenic or BAC transgenic mice. Services are available in multiple strains. We guarantee production of a minimum of 3 founders. Founder breeding and line characterization services are available. Pronuclear injection is also available for transgenic or knockout rat production.

We can also produce mutant mice or rats using CRISPR/Cas. Contact us for details.

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BAC Transgenic Mouse Production

Price on request

We offer pronuclear microinjection of bacterial artificial chromosomes. Injection available in C57BL/6, FVB or hybrid strains. Custom strains available upon request. At least 3 founders guaranteed! We offer a full suite of BAC recombineering services, BAC transgene validation and purification for microinjection. We handle the founder genotyping and offer founder breeding if required.

We offer pronuclear microinjection of bacterial artificial chromosomes. Injection available in C57BL/6, FVB or hybrid strains. Custom strains available upon request. At least 3 founders guaranteed! We offer a full suite of BAC recombineering services, BAC transgene validation and purification for microinjection. We handle the founder genotyping and offer founder breeding if required.

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Transgenic Rat Development

Price on request

We offer rat pronuclear injection services for production of transgenic or knockout rats.

We offer rat pronuclear injection services for production of transgenic or knockout rats.

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Pronuclear Injection

Price on request

We offer embryo microinjection for pruduction of transgenic animals or mutant strains using standard transgenes, BAC transgenes or engineered nucleases such as zinc finger nucleases and TALENs. Services available in mice and rats. Please inquire for details.

We offer embryo microinjection for pruduction of transgenic animals or mutant strains using standard transgenes, BAC transgenes or engineered nucleases such as zinc finger nucleases and TALENs. Services available in mice and rats. Please inquire for details.

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Embryonic Stem Cell (ESC) Culture

Price on request

We offer a full range of ES cell culture services including gene targeting, expansion, cryopreservation, chromosome analysis and preparation for blastocyst injection.

We offer a full range of ES cell culture services including gene targeting, expansion, cryopreservation, chromosome analysis and preparation for blastocyst injection.

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Embryonic Stem Cell Electroporation

Price on request

We offer a full range of ES cell electroporation and gene targeting services using a variety of ES cell strains.

We offer a full range of ES cell electroporation and gene targeting services using a variety of ES cell strains.

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Embryonic Stem Cell Gene Targeting

Price on request

We offer a full range of services for gene targeting for production of knockout or knockin mutations in ES cells and other cell lines. Services can be performed using vectors we generate or client-provided vectors.

We offer a full range of services for gene targeting for production of knockout or knockin mutations in ES cells and other cell lines. Services can be performed using vectors we generate or client-provided vectors.

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Embryonic Stem Cell Screening

Price on request

We offer a full range of ES cell screening services including long range PCR and Southern blotting. Assay design and development options available.

We offer a full range of ES cell screening services including long range PCR and Southern blotting. Assay design and development options available.

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Blastocyst Injection

Price on request

We offer blastocyst injection in C57BL/6 and C57BL/6-albino blastocysts for chimera production. Chimera breeding and Flpe-mediated selectable marker removal also available.

We can validate correct gene targeting of your KOMP/EUCOMM or other ES cells by Southern Blot. Service includes expansion and cryopreservation of the... Show more »

We offer blastocyst injection in C57BL/6 and C57BL/6-albino blastocysts for chimera production. Chimera breeding and Flpe-mediated selectable marker removal also available.

We can validate correct gene targeting of your KOMP/EUCOMM or other ES cells by Southern Blot. Service includes expansion and cryopreservation of the clones, allele validation by Southern blot and blastocyst injection of up to 3 clones for chimera production. Chimera breeding and Flpe-mediated marker removal also available.

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Embryonic Stem Cell Generation

Price on request

We offer derivation of mouse embryonic stem cell lines from customer-supplied mouse strains.

We offer derivation of mouse embryonic stem cell lines from customer-supplied mouse strains.

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Intracytoplasmic Sperm Injection

Price on request

Line recovery by ICSI using client-provided sperm samples.

Line recovery by ICSI using client-provided sperm samples.

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Animal Embryo and Sperm Cryopreservation

Price on request

We offer cryopreservation of mouse sperm or embryos. Cryorecovery by embryo transfer, IVF and ICSI is also available.

We offer cryopreservation of mouse sperm or embryos. Cryorecovery by embryo transfer, IVF and ICSI is also available.

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Rodent Rederivation

Price on request

We offer mouse strain rederivation by embryo transfer, IVF or ICSI. Animals will be transferred to client's institution from our restricted flora high barrier facility.

We offer mouse strain rederivation by embryo transfer, IVF or ICSI. Animals will be transferred to client's institution from our restricted flora high barrier facility.

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Molecular Cloning

Price on request

We offer traditional plasmid cloning as well as recombineering-based services for generation of transgene constructs, BAC constructs and gene targeting vectors.

We offer traditional plasmid cloning as well as recombineering-based services for generation of transgene constructs, BAC constructs and gene targeting vectors.

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Rodent in vitro Fertilization

Price on request

IVF services available for rederivation, speed expansion or cryorecovery.

IVF services available for rederivation, speed expansion or cryorecovery.

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Transgenic Genotyping

Price on request
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Animal Husbandry

Price on request

We offer mouse breeding and genotyping services.

We offer mouse breeding and genotyping services.

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Biospecimens

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Genotyping and Gene Expression Assays

Price on request
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Cells and Tissues

Price on request
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Animal Model Housing and Maintenance

Price on request
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Animal Biospecimens

Price on request
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Animal Models and Studies

Price on request
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Transgenic Mouse Services

Price on request
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Biochemistry & Molecular Biology

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Biology

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Mammalian Cell Culture

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Genetic Engineering

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DNA Services

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Nucleic Acid Services

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Dale Cowley

Director

Animal Models Core has not received any reviews.

Animal Models Core has not received any endorsements.