1352856160859 a2336ed1c2cc707a7d464a3906810725

Animal Models Core

Chapel Hill, North Carolina, US

The UNC Animal Models Core Facility provides the full range of mouse genetic modification services, reagents and related technologies. The Core's services are available to both UNC and Non-UNC clients.

Transgenic (pronuclear injection) animals can be generated in C57BL/6, FVB or other strains. Services for creating transgenic mice include transgene design, construct generation, isolation of the transgene fragment, pronuclear microinjection, founder genotyping and breeding.

The core also offers the full suite of services for BAC (bacterial artificial chromosome) recombineering and microinjection of BAC transgenes.

Gene targeting services are available in ES cells from C57BL/6, 129P2/Ola or BALB/c strains. Other strains available upon request. Gene targeting services include design and cloning of targeting vectors, creating gene-targeted ES cells, blastocyst microinjection of the ES cells to create... Show more »

The UNC Animal Models Core Facility provides the full range of mouse genetic modification services, reagents and related technologies. The Core's services are available to both UNC and Non-UNC clients.

Transgenic (pronuclear injection) animals can be generated in C57BL/6, FVB or other strains. Services for creating transgenic mice include transgene design, construct generation, isolation of the transgene fragment, pronuclear microinjection, founder genotyping and breeding.

The core also offers the full suite of services for BAC (bacterial artificial chromosome) recombineering and microinjection of BAC transgenes.

Gene targeting services are available in ES cells from C57BL/6, 129P2/Ola or BALB/c strains. Other strains available upon request. Gene targeting services include design and cloning of targeting vectors, creating gene-targeted ES cells, blastocyst microinjection of the ES cells to create transmitting chimeras and chimera breeding for germline transmission.

New in 2013: we now offer genome modification services using CRISPR/Cas nucleases. We can produce and validated the nucleases, then use them to generate knockout or knock-in mice, rats or cell lines.

The core can also provide Southern blot validation and blastocyst microinjection of ES cells from EUCOMM, KOMP or other consortia.

Additional services include Cre- or Flp-mediated excision in ES cells or in vivo, chromosome counting, PCR screening and Southern blotting.

Rat transgenic or knockou/knock-in model production is also available.

« Show less

Animal Models Core has not listed any services.

Recombineering
Recombination-Mediated Genetic Engineering
Price on request
Request a quote for more information about this service.
Transgenic Animal Model Development
Price on request

CRISPR/Cas nucleases can be designed to modify the genome, facilitating production of knockout or knock-in mice, rats or cell lines. Mouse and rat models can be produced in as little as 4 months! Contact us for a custom project quote.

CRISPR/Cas nucleases can be designed to modify the genome, facilitating production of knockout or knock-in mice, rats or cell lines. Mouse and rat models can be produced in as little as 4 months! Contact us for a custom project quote.

« Show less
Knockout Mouse Development
Price on request

We offer a full range of services for production of knockout, knockin or conditional knockout/knockin animals.

Knockout, conditional knockout and knockin mice can be custom designed to meet your project goals. Our full-service model production package includes design, targeting vector construction, ES cell electroporation, ES... Show more »

We offer a full range of services for production of knockout, knockin or conditional knockout/knockin animals.

Knockout, conditional knockout and knockin mice can be custom designed to meet your project goals. Our full-service model production package includes design, targeting vector construction, ES cell electroporation, ES cell screening, Southern blot validation, blastocyst injection and chimera breeding. Our service offerings are flexible to perform any parts of the process to meet individual client needs.

Knockout or knockin mice and rats can also be produced by microinjection of CRISPR/Cas engineered nucleases. We offer nuclease production and validation services in addition to microinjection. Contact us for details and pricing.

« Show less
Genetically Engineered Mice
Price on request

We offer a full range of services for production of knockout, knockin, transgenic and BAC transgenic animals.

Knockout, conditional knockout and knockin mice can be custom designed to meet your project goals. Our full-service model production package includes design, targeting vector construction, ES cell electroporation, ES... Show more »

We offer a full range of services for production of knockout, knockin, transgenic and BAC transgenic animals.

Knockout, conditional knockout and knockin mice can be custom designed to meet your project goals. Our full-service model production package includes design, targeting vector construction, ES cell electroporation, ES cell screening, Southern blot validation, blastocyst injection and chimera breeding. Our service offerings are flexible to perform any parts of the process to meet individual client needs.

Pronuclear microinjection services are available for production of transgenic or BAC transgenic mice. Services are available in multiple strains. We guarantee production of a minimum of 3 founders. Founder breeding and line characterization services are available. Pronuclear injection is also available for transgenic or knockout rat production.

We can also produce mutant mice or rats using CRISPR/Cas. Contact us for details.

« Show less
BAC Transgenic Mouse Production
Price on request

We offer pronuclear microinjection of bacterial artificial chromosomes. Injection available in C57BL/6, FVB or hybrid strains. Custom strains available upon request. At least 3 founders guaranteed! We offer a full suite of BAC recombineering services, BAC transgene validation and purification for microinjection. We handle the founder genotyping and offer founder breeding if required.

We offer pronuclear microinjection of bacterial artificial chromosomes. Injection available in C57BL/6, FVB or hybrid strains. Custom strains available upon request. At least 3 founders guaranteed! We offer a full suite of BAC recombineering services, BAC transgene validation and purification for microinjection. We handle the founder genotyping and offer founder breeding if required.

« Show less
Transgenic Rat Development
Price on request

We offer rat pronuclear injection services for production of transgenic or knockout rats.

We offer rat pronuclear injection services for production of transgenic or knockout rats.

« Show less
Pronuclear Injection
Price on request

We offer embryo microinjection for pruduction of transgenic animals or mutant strains using standard transgenes, BAC transgenes or engineered nucleases such as zinc finger nucleases and TALENs. Services available in mice and rats. Please inquire for details.

We offer embryo microinjection for pruduction of transgenic animals or mutant strains using standard transgenes, BAC transgenes or engineered nucleases such as zinc finger nucleases and TALENs. Services available in mice and rats. Please inquire for details.

« Show less
Embryonic Stem Cell (ESC) Culture
Price on request

We offer a full range of ES cell culture services including gene targeting, expansion, cryopreservation, chromosome analysis and preparation for blastocyst injection.

We offer a full range of ES cell culture services including gene targeting, expansion, cryopreservation, chromosome analysis and preparation for blastocyst injection.

« Show less
Embryonic Stem Cell Electroporation
Price on request

We offer a full range of ES cell electroporation and gene targeting services using a variety of ES cell strains.

We offer a full range of ES cell electroporation and gene targeting services using a variety of ES cell strains.

« Show less
Embryonic Stem Cell Gene Targeting
Price on request

We offer a full range of services for gene targeting for production of knockout or knockin mutations in ES cells and other cell lines. Services can be performed using vectors we generate or client-provided vectors.

We offer a full range of services for gene targeting for production of knockout or knockin mutations in ES cells and other cell lines. Services can be performed using vectors we generate or client-provided vectors.

« Show less
Embryonic Stem Cell Screening
Price on request

We offer a full range of ES cell screening services including long range PCR and Southern blotting. Assay design and development options available.

We offer a full range of ES cell screening services including long range PCR and Southern blotting. Assay design and development options available.

« Show less
Blastocyst Injection
Price on request

We offer blastocyst injection in C57BL/6 and C57BL/6-albino blastocysts for chimera production. Chimera breeding and Flpe-mediated selectable marker removal also available.

We can validate correct gene targeting of your KOMP/EUCOMM or other ES cells by Southern Blot. Service includes expansion and cryopreservation of the... Show more »

We offer blastocyst injection in C57BL/6 and C57BL/6-albino blastocysts for chimera production. Chimera breeding and Flpe-mediated selectable marker removal also available.

We can validate correct gene targeting of your KOMP/EUCOMM or other ES cells by Southern Blot. Service includes expansion and cryopreservation of the clones, allele validation by Southern blot and blastocyst injection of up to 3 clones for chimera production. Chimera breeding and Flpe-mediated marker removal also available.

« Show less
Embryonic Stem Cell Generation
Price on request

We offer derivation of mouse embryonic stem cell lines from customer-supplied mouse strains.

We offer derivation of mouse embryonic stem cell lines from customer-supplied mouse strains.

« Show less
Intracytoplasmic Sperm Injection
Price on request

Line recovery by ICSI using client-provided sperm samples.

Line recovery by ICSI using client-provided sperm samples.

« Show less
Animal Embryo and Sperm Cryopreservation
Price on request

We offer cryopreservation of mouse sperm or embryos. Cryorecovery by embryo transfer, IVF and ICSI is also available.

We offer cryopreservation of mouse sperm or embryos. Cryorecovery by embryo transfer, IVF and ICSI is also available.

« Show less
Rodent Rederivation
Price on request

We offer mouse strain rederivation by embryo transfer, IVF or ICSI. Animals will be transferred to client's institution from our restricted flora high barrier facility.

We offer mouse strain rederivation by embryo transfer, IVF or ICSI. Animals will be transferred to client's institution from our restricted flora high barrier facility.

« Show less
Mouse
Molecular Cloning
Price on request

We offer traditional plasmid cloning as well as recombineering-based services for generation of transgene constructs, BAC constructs and gene targeting vectors.

We offer traditional plasmid cloning as well as recombineering-based services for generation of transgene constructs, BAC constructs and gene targeting vectors.

« Show less
Rodent in vitro Fertilization
Price on request

IVF services available for rederivation, speed expansion or cryorecovery.

IVF services available for rederivation, speed expansion or cryorecovery.

« Show less
Transgenic Genotyping
Price on request
Request a quote for more information about this service.
Animal Husbandry
Price on request

We offer mouse breeding and genotyping services.

We offer mouse breeding and genotyping services.

« Show less
Animal Models and Studies
Price on request
Request a quote for more information about this service.
Animal Model Housing and Maintenance
Price on request
Request a quote for more information about this service.
Animal Biospecimens
Price on request
Request a quote for more information about this service.
Transgenic Mouse Services
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.
Cells and Tissues
Price on request
Request a quote for more information about this service.
Biospecimens
Price on request
Request a quote for more information about this service.
Mammalian Cell Culture
Price on request
Request a quote for more information about this service.
Genetic Engineering
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.
DNA Services
Price on request
Request a quote for more information about this service.
Genotyping Services
Price on request
Request a quote for more information about this service.

Animal Models Core has not received any ratings.

Be the first to endorse Animal Models Core

Endorse this lab