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Animal Health Diagnostic Center

Ithaca, NY, US

The Animal Health Diagnostic Center (AHDC) at Cornell University offers a wide array of testing capabilities to detect markers of health and disease in animals. It is one of the most comprehensive veterinary diagnostic laboratories in the country, whose faculty provide subject matter expertise and oversight of more than 900 individual assays, performed by 200 highly trained technical staff.

The AHDC services include large and small-scale testing for academic and industry research and development. Our sophisticated and comprehensive diagnostic capabilities include anatomic pathology (necropsy and histopathology services), clinical pathology (hematology, hemostasis, cytology, endocrinology), nutritional and toxicology testing. Infectious disease sections span all relevant disciplines including bacteriology, mycology, parasitology, and virology with a wide range of culture capabilities and available cell lines. The... Show more »

The Animal Health Diagnostic Center (AHDC) at Cornell University offers a wide array of testing capabilities to detect markers of health and disease in animals. It is one of the most comprehensive veterinary diagnostic laboratories in the country, whose faculty provide subject matter expertise and oversight of more than 900 individual assays, performed by 200 highly trained technical staff.

The AHDC services include large and small-scale testing for academic and industry research and development. Our sophisticated and comprehensive diagnostic capabilities include anatomic pathology (necropsy and histopathology services), clinical pathology (hematology, hemostasis, cytology, endocrinology), nutritional and toxicology testing. Infectious disease sections span all relevant disciplines including bacteriology, mycology, parasitology, and virology with a wide range of culture capabilities and available cell lines. The molecular diagnostics laboratory has high-throughput capacity for identifying defined and novel pathogenic and non-pathogenic organisms, mutation detection for genetic disease, and whole genome sequencing of bacterial isolates.

The AHDC excels in assay customization and integrated diagnostic support, with flexibility to meet the needs of clients engaged in basic and applied research and development.

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Animal Health Diagnostic Center has not listed any services.

Animal Molecular Diagnostic Testing
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Clinical Endocrinology Tests
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Comparative Endocrinology Laboratory

Our laboratory offers research testing and immunoassay services, which include assay set up, development, and validation for a variety of hormones. The laboratory has an extensive array of hormones that have been assayed over the years, using immunoassay technologies (i.e. RIA, CIA and... Show more »

Comparative Endocrinology Laboratory

Our laboratory offers research testing and immunoassay services, which include assay set up, development, and validation for a variety of hormones. The laboratory has an extensive array of hormones that have been assayed over the years, using immunoassay technologies (i.e. RIA, CIA and ELISA) on samples from many species. Our immunoassay service and expertise supports research at universities, non-profit foundations and institutes, and pharmaceutical and life science industries.

The Quality Assurance system, initially established within the Endocrinology Lab, used Good Laboratory Practices (CFR 21 Part 58) as guidelines to maintain quality and ensure data integrity. The laboratory currently has adapted their system to follow the QA program established within the AHDC, necessary for accreditation with AAVLD. The laboratory voluntarily participates in the Society for Comparative Endocrinology and Veterinary Laboratory Association proficiency testing programs.

Please contact the Endocrinology Laboratory manager (607) 253-3593 or director (607) 253-3796 to review the specifics of your testing needs.

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Goat
Dog
Horse
Cat
Biology
serum
plasma
ELISA
Hyperthyroidism
Hypothyroidism
Exotic species
Equine cushing's
Equine metabolic syndrome
Reproductive conditions
Immulites
Gamma counters
Bio Tek Synergy Microplate reader
Chemiluminescent Immunoassay
Radioimmunoassay
Camel
Cattle
Coagulation Profile
Price on request

Comparative Coagulation Laboratory

A veterinary referral laboratory offering comprehensive hemostasis testing for animal species. We provide customized assays, flexible report formats, and pre-submission consultation.

• Preclinical testing for pharmaceutical and contract research organizations
• Hemostasis assays for... Show more »

Comparative Coagulation Laboratory

A veterinary referral laboratory offering comprehensive hemostasis testing for animal species. We provide customized assays, flexible report formats, and pre-submission consultation.

• Preclinical testing for pharmaceutical and contract research organizations
• Hemostasis assays for biomedical and veterinary researchers
• GLP and non-GLP testing
• Test evaluation and optimization
• Comprehensive test menu including:
o Procoagulant factors: Coagulation factor activity assays: FII through XII, Factor XIII
o Hemostatic proteins: Fibrinogen, Von Willebrand factor
o Anticoagulants: antithrombin, protein C, heparin and low molecular weight heparin, direct anti-Xa inhibitor assays
o Fibrinolysis:, Plasminogen, antiplasmin, PAI-1, D-dimer, microplate fibrinolysis screen
o Thrombin generation assays: Thrombinoscope
o Thrombelastographic assays: Plasma coagulation and fibrinolysis
o Platelet flow cytometry

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Rabbit
Sheep
Goat
Mouse
Pig
Rat
Dog
Horse
Cat
Birds
Exotic species
Wildlife species
Anticoagulants
Procoagulant proteins
Fibrinolytic proteins
Platelets
Bleeding disorders
Thrombotic disease
Platelet disorders
Coagulation Factors
Non-human primates
Veterinary Microbiology & Parasitology
Price on request

The Animal Health Diagnostic Center is unique among AAVLD accredited lab in having parasitology as a stand-alone section with full-service veterinary parasite diagnostic capabilities. Overseen by a board certified parasitologist, the section offers classical, serological and molecular services for detection/identification of... Show more »

The Animal Health Diagnostic Center is unique among AAVLD accredited lab in having parasitology as a stand-alone section with full-service veterinary parasite diagnostic capabilities. Overseen by a board certified parasitologist, the section offers classical, serological and molecular services for detection/identification of parasites of veterinary importance and diagnosing parasitic infections in domestic and wild animals. This service is provided in an accurate, timely and cost efficient manner.
Host species tested include bovine, equine, caprine, ovine, canine, feline, equine, camelids, avian, lab animals, exotic, zoo animals, wild animals and birds, fishes, marine mammals.
Samples typically received for examination include feces, entire endo- and ectoparasites, necropsy samples, skin scrapings, blood, serum, plasma, urine, tracheal washes, duodenal aspirates, feed samples, and environmental samples such as soil and composted material.
Tests conducted to identify parasites or parasite stages in feces include:
• Qualitative or quantitative centrifugation concentration flotation techniques
- We employ a comprehensive evaluation strategy based on host species and age group of animal tested and consistently use two different floatation solutions of varied specific gravity (Sugar and Zinc Sulphate) to maximize the recovery of nematode, cestode, trematode and protozoan parasite stages.
• Antigen capture ELISA tests for Giardia sp. and Cryptosporidium sp.
- This procedure is done in parallel with double centrifugation floatation for accurate evaluation of disease status in the host, all in one price.
• Modified Baermann technique for recovery of nematode larvae
- This test is used to retrieve some species of lungworm larvae in domestic and pet animals. Also used to retrieve protostrongylid larvae from infected wild ungulates. This can be combined with molecular confirmation for accurate species identification.
• Nematode larvae cultures
- Used to determine the species of “strongyle” nematodes by culturing the eggs (which are indistinguishable between various strongyles) in the feces to obtain third stage larvae which can then be identified further.

Tests conducted to identify parasitic infections using serum/plasma include:
• Occult heartworm test
- This test is used for the detection of adult female Dirofilaria immitis antigen in canine and feline serum or plasma. This test is validated for use in dogs and cats only. Other animals may be tested by this method, but reactivity in other species is not clearly defined.
• Toxoplasma IgG ELISA
- The Toxoplasma gondii IgG ELISA tests serum for the presence of anti-Toxoplasma IgG antibody. This test is valid to test serum from a broad range of animal species, except for birds, marsupials and cold-blooded animals.
• Feline antibody heartworm test
- This test detects antibodies resulting from heartworm infections in cats that may include immature worms, adult male and female worms or single sex male or female infections. This test is validated for samples from domestic feline hosts only.

Other tests conducted include:
• Parasite identification
- Whole parasites, on histological slides, in a biopsy etc., are processed by morphological and/or molecular methods.
• Fecal Egg Count Reduction Test (FECRT)
- Done as herd evaluation to check for anthelmintic (anti-parasitic drug) resistance
• Tritrichomonas fetus InPouch culture test
- InPouch culture of preputial wash from bulls to detect Tritrichomonas fetus
- InPouch™ TF method is used for the diagnosis of Tritrichomonas foetus infection in cats
• Knott’s technique for microfilariae in blood
• KOH digestion of skin scrapings to retrieve ectoparasites
• Whirling Disease testing of fish

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Veterinary Pathology
Price on request

Our laboratory is equipped with state-of-the-art equipment and facilities including high-throughput automated extraction and liquid handling systems and a variety of platforms for PCR and next-generation sequencing. We operate within a Quality Assurance System that complies with the American Association of Veterinary Laboratory... Show more »

Our laboratory is equipped with state-of-the-art equipment and facilities including high-throughput automated extraction and liquid handling systems and a variety of platforms for PCR and next-generation sequencing. We operate within a Quality Assurance System that complies with the American Association of Veterinary Laboratory Diagnosticians. The lab also undergoes proficiency testing conducted by the National Veterinary Services Laboratory and the US Food and Drug Administration.

Services Provided:
• Pathogen load in animal specimens by qPCR using the ABI 7500 and OpenArray platforms
• Whole genome sequencing of bacterial isolates on the Illumina MiSeq platform
• Environmental surveillance for Salmonella using enrichment rtPCR
• Tick testing for up to 14 different pathogens by qPCR
• Digital PCR on the Biorad QX200 ddPCR system

Typical sample requirements:
• Feces weighed into 400mg aliquots in snap-cap microcentrifuge tubes and frozen at -80
• Respiratory swabs in sterile, sealed vials with several drops of saline added
• Ticks frozen or preserved in ethanol
• Contact us for other animal or environmental specimen types

Deliverables
• qPCR/rtPCR results will be provided in Excel spreadsheets with Ct values (and copy number
interpolations if requested)
• Bacterial WGS - fastq files and/or draft genomes provided by FTP or uploaded to NCBI

Recent publications highlighting our work:
• Wagner et al. Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age. PLoS One 2017 http://dx.plos.org/10.1371/journal.pone.0169072
• Goodman LB et al. High-throughput detection of respiratory pathogens in animal specimens by nanoscale PCR. Journal of Visualized Experiments. 2016 Nov 28;(117) http://bit.ly/2gRLsn0

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Applied Biosystems 7500 Fast Real-Time PCR system
Goat
Coronavirus
Dog
Horse
Bio-Rad QX200 droplet digital PCR
Cat
KingFisher FLEX
QuantStudio 12K Flex
qPCR
WGS
Lyme disease
Frozen Tissue
csf
feces
Influenza A
EDTA Whole Blood
Swab
Cryptosporidiosis
Leptospirosis
Salmonellosis
Applied Biosystems OpenArray
ddPCR
real-time PCR
tick
avian
Johne's disease (Mycobacterium avium ss. paratuberculosis)
Bovine respiratory disease complex
canine respiratory disease complex
feline respiratory disease complex
tick-borne diseases
Cattle
Clinical Research
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Clinical Laboratory Services
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Veterinary Laboratory Services
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Veterinary Research & Diagnostic Services
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Clinical Hematology
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