The Angio BioCore (ABC) is a state-of-the-art facility that has been established through the Indiana University Melvin and Bren Simon Cancer Center, a National Cancer Institute-designated cancer center, to conduct validated and highly reproducible in vitro and in vivo angiogenesis, endothelial, hematopoietic and multi-parametric flow cytometry assays and their role in normal and patient-related hematologic and cardiovascular disorders.
In addition to possessing the in vitro and in vivo assays that define the endothelial progenitor outgrowth cells that possess in vivo vessel forming ability, the ABC recently stringently defined a population of pro-angiogenic and anti-angiogenic circulating hematopoietic stem and progenitor cells (CHSPCs) that has been shown to regulate angiogenesis. Discovery of these novel CHSPC subsets demonstrates the uniqueness and strength of the approach by the ABC that requires both... Show more »
Angio BioCore (ABC) has not listed any services.
Proliferation assays require cells and compounds from the researchers ordering the experiment. Expected data varies with which assay is performed.
Turnaround time: ~2 weeks
1x6 EOCs derived from either peripheral blood or human umbilical cord blood (p4-p5).
$340 USD per Sample
Expansion of EOC colonies to passage 2 and cryopreservation of cells.
$182.00 USD per Sample
Quantitation of EOCs from human blood:
Plating, maintenance, and quantitation of 3 wells for EOCs
Isolation of MNCs is a... Show more »
1e6 human unbilical cord blood CD34+ cells.
Tested for sterility, purity (>95%), and functionality (CFU-GEMMs).
Assessment of Endothelial Cell Apoptosis via TUNEL:
TUNEL assessment of endothelial cell apoptosis.
Up to 6 conditions in triplicate, including controls.
Does not include cost of cell line.
$900.00 USD per Sample
Turnaround time: ~2 weeks
Endothelial Cell Migration Assays:
Haptotaxis in transwells for up to 8 conditions (including controls) in triplicate.
Does not include cost of cell line.
$665.00 USD per Sample
Turnaround time: ~2 weeks
Staining of cell line with endothelial antigen panel to confirm phenotype, including controls
Does not include usage charge from Flow Cytometry Core
Turnaround time: ~2 weeks
Endothelial cell isolation from human umbilical veins:
Isolation of endothelial cells from human umbilical veins, expansion to passage 1 and phenotypic confirmation by multi-parametric flow cytometry.
$700.00 USD per Sample
Turnaround time: ~2 weeks
Preparation including underlaying of up to 100mls of blood, washes and cell count. $157 USD per Sample
Preparation including underlaying of up to 20mls of blood, washes and cell count $82 USD per Sample
Preparation includes processing of up to 48mls of blood in CPT tubes supplied by the user, washes and cell count $100 USD per Sample
7-Color Multi-Parametric Flow Cytometric Analysis of Human Blood MNCs:
Staining of MNCs with 7 antigen panel (CD14, CD16, CD31, CD34, CD45, AC133, LIVEDEAD), including necessary FMO controls and viability markers, and brief analysis in FlowJo.
Requires a blood sample or cell line from the researcher ordering the... Show more »
Plasma Separation and Freeze Down:
Preparation includes centrifuging and freeze down of up to 8 vials of plasma.
$12 USD per Sample
MNC Separation and Freeze Down:
Preparation includes isolation of MNCs and freeze down of up to 5 vials of MNCs
$82.00 USD per Sample
Plating, culture and quantitation of CFU-GEMMs (in triplicate)
Isolation of MNCs is a separate charge
Turnaround time: ~2 weeks
Up to 10 conditions in triplicate assayed for endothelial cell tube formation.
Does not include cost of cell line.
A tube-forming assay requires cells either provided from the researcher or purchased through the Angio BioCore and compounds from the researchers ordering the experiment. Expected data varies with which assay is... Show more »
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