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Angio BioCore (ABC)

Indianapolis, Indiana, US

The Angio BioCore (ABC) is a state-of-the-art facility that has been established through the Indiana University Melvin and Bren Simon Cancer Center, a National Cancer Institute-designated cancer center, to conduct validated and highly reproducible in vitro and in vivo angiogenesis, endothelial, hematopoietic and multi-parametric flow cytometry assays and their role in normal and patient-related hematologic and cardiovascular disorders.

In addition to possessing the in vitro and in vivo assays that define the endothelial progenitor outgrowth cells that possess in vivo vessel forming ability, the ABC recently stringently defined a population of pro-angiogenic and anti-angiogenic circulating hematopoietic stem and progenitor cells (CHSPCs) that has been shown to regulate angiogenesis. Discovery of these novel CHSPC subsets demonstrates the uniqueness and strength of the approach by the ABC that requires both... Show more »

The Angio BioCore (ABC) is a state-of-the-art facility that has been established through the Indiana University Melvin and Bren Simon Cancer Center, a National Cancer Institute-designated cancer center, to conduct validated and highly reproducible in vitro and in vivo angiogenesis, endothelial, hematopoietic and multi-parametric flow cytometry assays and their role in normal and patient-related hematologic and cardiovascular disorders.

In addition to possessing the in vitro and in vivo assays that define the endothelial progenitor outgrowth cells that possess in vivo vessel forming ability, the ABC recently stringently defined a population of pro-angiogenic and anti-angiogenic circulating hematopoietic stem and progenitor cells (CHSPCs) that has been shown to regulate angiogenesis. Discovery of these novel CHSPC subsets demonstrates the uniqueness and strength of the approach by the ABC that requires both phenotypic and functional data to validate specific circulating cells that participate in new blood vessel formation.

The specific assays offered by the ABC function as quantitative analytical tools, potential biomarkers of several hematopoietic diseases, and as experimental platforms for understanding the basic mechanisms of angiogenesis and the interplay between the endothelial and hematopoietic systems. For example, not only are CHSPCs critical for normal and abnormal angiogenesis, but we and others have reported that certain endothelial cells are critical for CHSPC expansion ex vivo and that endothelial cells promote CHSPC engraftment post-ablation.

The ABC serves to directly perform all of the assays required to analyze research samples, and as a consultation, education, and new assay development site for scientists within and outside the IU School of Medicine and Indiana University.

Furthermore, the ABC is one of only five nationally recognized Core Centers of Excellence in Molecular Hematology by the National Institutes of Health and is a certified core of the Indiana Clinical and Translational Sciences Institute (CTSI).

ABC has access to various mouse models through another Core facility here at Indiana University. We are also happy to order any cell lines.


Lab staff:
Jamie Case, PhD.; Emily Sims, BS; Matthew Repass, BS; Julie Mund, MS.

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Biomarker Analysis
Price on request
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Flow cytometry
Cell Proliferation Assays
Price on request

Proliferation assays require cells and compounds from the researchers ordering the experiment. Expected data varies with which assay is performed.

Turnaround time: ~2 weeks

Proliferation assays require cells and compounds from the researchers ordering the experiment. Expected data varies with which assay is performed.

Turnaround time: ~2 weeks

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Cryopreserved EOC cell line
Starting at $182.00 per sample

1x6 EOCs derived from either peripheral blood or human umbilical cord blood (p4-p5).
$340 USD per Sample

Expansion of EOC colonies to passage 2 and cryopreservation of cells.
$182.00 USD per Sample

Quantitation of EOCs from human blood:
Plating, maintenance, and quantitation of 3 wells for EOCs
Isolation of MNCs is a... Show more »

1x6 EOCs derived from either peripheral blood or human umbilical cord blood (p4-p5).
$340 USD per Sample

Expansion of EOC colonies to passage 2 and cryopreservation of cells.
$182.00 USD per Sample

Quantitation of EOCs from human blood:
Plating, maintenance, and quantitation of 3 wells for EOCs
Isolation of MNCs is a separate charge
$300.00 USD per Sample

Turnaround time: ~2-4 weeks

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Cryopreserved HMVEC cell line
Starting at $450.00 per sample

1e6 cells.

1e6 cells.

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Cryopreserved HUVEC cell line
Starting at $225.00 per sample

1e6 cells.

1e6 cells.

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Cryopreserved Human CD34+ Cells
Starting at $750.00 per sample

1e6 human unbilical cord blood CD34+ cells.
Tested for sterility, purity (>95%), and functionality (CFU-GEMMs).

1e6 human unbilical cord blood CD34+ cells.
Tested for sterility, purity (>95%), and functionality (CFU-GEMMs).

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Cryopreserved PMVEC cell line
Starting at $525.00 per sample

1e6 cells.

1e6 cells.

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Apoptosis Assays
Starting at $900.00 per sample

Assessment of Endothelial Cell Apoptosis via TUNEL:
TUNEL assessment of endothelial cell apoptosis.
Up to 6 conditions in triplicate, including controls.
Does not include cost of cell line.
$900.00 USD per Sample

Turnaround time: ~2 weeks

Assessment of Endothelial Cell Apoptosis via TUNEL:
TUNEL assessment of endothelial cell apoptosis.
Up to 6 conditions in triplicate, including controls.
Does not include cost of cell line.
$900.00 USD per Sample

Turnaround time: ~2 weeks

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Cell Migration Assays
Starting at $665.00 per sample

Endothelial Cell Migration Assays:
Haptotaxis in transwells for up to 8 conditions (including controls) in triplicate.
Does not include cost of cell line.
$665.00 USD per Sample

Turnaround time: ~2 weeks

Endothelial Cell Migration Assays:
Haptotaxis in transwells for up to 8 conditions (including controls) in triplicate.
Does not include cost of cell line.
$665.00 USD per Sample

Turnaround time: ~2 weeks

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Cellular Phenotype Confirmation
Starting at $182.00 per sample

Staining of cell line with endothelial antigen panel to confirm phenotype, including controls
Does not include usage charge from Flow Cytometry Core

Turnaround time: ~2 weeks

Staining of cell line with endothelial antigen panel to confirm phenotype, including controls
Does not include usage charge from Flow Cytometry Core

Turnaround time: ~2 weeks

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Endothelial cell isolation
Starting at $700.00 per sample

Endothelial cell isolation from human umbilical veins:
Isolation of endothelial cells from human umbilical veins, expansion to passage 1 and phenotypic confirmation by multi-parametric flow cytometry.
$700.00 USD per Sample

Turnaround time: ~2 weeks

Endothelial cell isolation from human umbilical veins:
Isolation of endothelial cells from human umbilical veins, expansion to passage 1 and phenotypic confirmation by multi-parametric flow cytometry.
$700.00 USD per Sample

Turnaround time: ~2 weeks

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PBMC Isolation
Peripheral Blood Mononuclear Cell Isolation
Starting at $82.00 per sample

Preparation including underlaying of up to 100mls of blood, washes and cell count. $157 USD per Sample

Preparation including underlaying of up to 20mls of blood, washes and cell count $82 USD per Sample

Preparation includes processing of up to 48mls of blood in CPT tubes supplied by the user, washes and cell count $100 USD per Sample

Preparation including underlaying of up to 100mls of blood, washes and cell count. $157 USD per Sample

Preparation including underlaying of up to 20mls of blood, washes and cell count $82 USD per Sample

Preparation includes processing of up to 48mls of blood in CPT tubes supplied by the user, washes and cell count $100 USD per Sample

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Flow Cytometry
Starting at $250.00 per sample

7-Color Multi-Parametric Flow Cytometric Analysis of Human Blood MNCs:
Staining of MNCs with 7 antigen panel (CD14, CD16, CD31, CD34, CD45, AC133, LIVEDEAD), including necessary FMO controls and viability markers, and brief analysis in FlowJo.
Requires a blood sample or cell line from the researcher ordering the... Show more »

7-Color Multi-Parametric Flow Cytometric Analysis of Human Blood MNCs:
Staining of MNCs with 7 antigen panel (CD14, CD16, CD31, CD34, CD45, AC133, LIVEDEAD), including necessary FMO controls and viability markers, and brief analysis in FlowJo.
Requires a blood sample or cell line from the researcher ordering the experiment.
Deliverables:
- percentage data in an Excel sheet
- flow files
Does not include cost of data collection in Flow Cytometry Core.
Isolation of MNCs is a separate charge.
$250.00 USD per Sample


Up to 8-Color Analysis of Human Blood MNCs, Bone Marrow MNCs or Cell Lines:
Can design any panel for the specific PI's interests.
Staining of MNCs with 8 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
Does not include usage charge from Flow Cytometry Facility.
Isolation of cells is a separate charge.
$350.00 USD per Sample


Up to 8-Color Analysis of Preclinical (murine/rat/canine/monkey) Blood MNCs, Bone Marrow MNCs or Cell Lines:
Can design any panel for the specific PI's interests.
Staining of MNCs with 8 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
Does not include usage charge from Flow Cytometry Facility.
Isolation of cells is a separate charge.
Price on request.


Up to 8-Color Cell Sorting of Preclinical (murine/rat/canine/monkey) or Human Blood MNCs, Bone Marrow MNCs or Cell Lines:
Can design any panel for the specific PI's interests.
Staining of MNCs with 8 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo and set up on sorter of desired populations.
Does not include usage charge from Flow Cytometry Facility.
Isolation of MNCs is a separate charge.
Price on request.


Retrospective analysis of FCS3.0 files:
Detailed/retrospective flow cytometry analysis utilizing FloJo
-Data mining/analysis/re-analysis of previously acquired list mode data (FCS3.0 files) of EPC or CHSPC phenotyping for newly identified cell sub-populations of interest.
$100.00 USD per Hour


Protocol design and development:
Project specific consulting for investigators interested in the implementation of flow cytometry utilizing EPCs and circulating hematopoietic stem and progenitor cells (CHSPCs) as biomarkers in pre-clinical and clinical phase applications.

$100.00 USD per Hour


Deliverables:
- percentage data in an Excel sheet

Turnaround time: ~2 weeks

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Plasma Storage
Starting at $12.00 per sample

Plasma Separation and Freeze Down:
Preparation includes centrifuging and freeze down of up to 8 vials of plasma.
$12 USD per Sample

MNC Separation and Freeze Down:
Preparation includes isolation of MNCs and freeze down of up to 5 vials of MNCs
$82.00 USD per Sample

Plasma Separation and Freeze Down:
Preparation includes centrifuging and freeze down of up to 8 vials of plasma.
$12 USD per Sample

MNC Separation and Freeze Down:
Preparation includes isolation of MNCs and freeze down of up to 5 vials of MNCs
$82.00 USD per Sample

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CFU-GEMM Cells
Myeloid Multipotential Progenitor Cells
Starting at $50.00 per sample

Plating, culture and quantitation of CFU-GEMMs (in triplicate)
Isolation of MNCs is a separate charge

Turnaround time: ~2 weeks

Plating, culture and quantitation of CFU-GEMMs (in triplicate)
Isolation of MNCs is a separate charge

Turnaround time: ~2 weeks

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Matrigel Plug Assay
Starting at $425.00 per sample

Up to 10 conditions in triplicate assayed for endothelial cell tube formation.
Does not include cost of cell line.

A tube-forming assay requires cells either provided from the researcher or purchased through the Angio BioCore and compounds from the researchers ordering the experiment. Expected data varies with which assay is... Show more »

Up to 10 conditions in triplicate assayed for endothelial cell tube formation.
Does not include cost of cell line.

A tube-forming assay requires cells either provided from the researcher or purchased through the Angio BioCore and compounds from the researchers ordering the experiment. Expected data varies with which assay is performed.

Deliverables:
- photos of your tubes
- quantitative data

Turnaround time: ~2 weeks

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Cryostorage
Price on request
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Cell Lines
Price on request
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Cell-Based Assays
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Angiogenesis & Endothelial Cell Assays
Price on request
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Biology
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Cells and Tissues
Price on request
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Biospecimens
Price on request
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Cell Invasion & Migration Assays
Price on request
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Primary Cell Culture
Price on request
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Cytometry
Price on request
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Biospecimen Management and Storage
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Pharmacology & Toxicology
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Bioanalysis
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Biomarkers
Price on request
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Cell Viability & Proliferation Assays
Price on request
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Cell Death Assays
Price on request
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Human Biospecimens
Price on request
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Human Whole Blood & PBMCs
Price on request
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October 8, 2013

Positive review received for Matrigel Plug Assay:

"Great communication. Pleasure to work with again."

September 25, 2013

Positive review received for Matrigel Plug Assay:

"Great experience. Responsive. THX"

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