Angio BioCore (ABC)
100% Positive
3 Orders Completed
Indianapolis, Indiana, US
The Angio BioCore (ABC) is a state-of-the-art facility that has been established through the Indiana University Melvin and Bren Simon Cancer Center, a National Cancer Institute-designated cancer center, to conduct validated and highly reproducible in vitro and in vivo angiogenesis, endothelial, hematopoietic and multi-parametric flow cytometry assays and their role in normal and patient-related hematologic and cardiovascular disorders.
In addition to possessing the in vitro and in vivo assays that define the endothelial progenitor outgrowth cells that possess in vivo vessel forming ability, the ABC recently stringently defined a population of pro-angiogenic and anti-angiogenic circulating hematopoietic stem and progenitor cells (CHSPCs) that has been shown to regulate angiogenesis. Discovery of these novel CHSPC subsets demonstrates the uniqueness and strength of the approach by the ABC that requires both phenotypic and functional data to validate specific circulating cells that participate in new blood vessel formation.
The specific assays offered by the ABC function as quantitative analytical tools, potential biomarkers of several hematopoietic diseases, and as experimental platforms for understanding the basic mechanisms of angiogenesis and the interplay between the endothelial and hematopoietic systems. For example, not only are CHSPCs critical for normal and abnormal angiogenesis, but we and others have reported that certain endothelial cells are critical for CHSPC expansion ex vivo and that endothelial cells promote CHSPC engraftment post-ablation.
The ABC serves to directly perform all of the assays required to analyze research samples, and as a consultation, education, and new assay development site for scientists within and outside the IU School of Medicine and Indiana University.
Furthermore, the ABC is one of only five nationally recognized Core Centers of Excellence in Molecular Hematology by the National Institutes of Health and is a certified core of the Indiana Clinical and Translational Sciences Institute (CTSI).
ABC has access to various mouse models through another Core facility here at Indiana University. We are also happy to order any cell lines.
Lab staff:
Jamie Case, PhD.; Emily Sims, BS; Matthew Repass, BS; Julie Mund, MS.
1x6 EOCs derived from either peripheral blood or human umbilical cord blood (p4-p5).
$340 USD per Sample
Expansion of EOC colonies to passage 2 and cryopreservation of cells.
$182.00 USD per Sample
Quantitation of EOCs from human blood:
Plating, maintenance, and quantitation of 3 wells for EOCs
Isolation of MNCs is a separate charge
$300.00 USD per Sample
Turnaround time: ~2-4 weeks
1e6 human unbilical cord blood CD34+ cells.
Tested for sterility, purity (>95%), and functionality (CFU-GEMMs).
Staining of cell line with endothelial antigen panel to confirm phenotype, including controls
Does not include usage charge from Flow Cytometry Core
Turnaround time: ~2 weeks
Endothelial cell isolation from human umbilical veins:
Isolation of endothelial cells from human umbilical veins, expansion to passage 1 and phenotypic confirmation by multi-parametric flow cytometry.
$700.00 USD per Sample
Turnaround time: ~2 weeks
Preparation including underlaying of up to 100mls of blood, washes and cell count. $157 USD per Sample
Preparation including underlaying of up to 20mls of blood, washes and cell count $82 USD per Sample
Preparation includes processing of up to 48mls of blood in CPT tubes supplied by the user, washes and cell count $100 USD per Sample
7-Color Multi-Parametric Flow Cytometric Analysis of Human Blood MNCs:
Staining of MNCs with 7 antigen panel (CD14, CD16, CD31, CD34, CD45, AC133, LIVEDEAD), including necessary FMO controls and viability markers, and brief analysis in FlowJo.
Requires a blood sample or cell line from the researcher ordering the experiment.
Deliverables:
Up to 8-Color Analysis of Human Blood MNCs, Bone Marrow MNCs or Cell Lines:
Can design any panel for the specific PI's interests.
Staining of MNCs with 8 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
Does not include usage charge from Flow Cytometry Facility.
Isolation of cells is a separate charge.
$350.00 USD per Sample
Up to 8-Color Analysis of Preclinical (murine/rat/canine/monkey) Blood MNCs, Bone Marrow MNCs or Cell Lines:
Can design any panel for the specific PI's interests.
Staining of MNCs with 8 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
Does not include usage charge from Flow Cytometry Facility.
Isolation of cells is a separate charge.
Price on request.
Up to 8-Color Cell Sorting of Preclinical (murine/rat/canine/monkey) or Human Blood MNCs, Bone Marrow MNCs or Cell Lines:
Can design any panel for the specific PI's interests.
Staining of MNCs with 8 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo and set up on sorter of desired populations.
Does not include usage charge from Flow Cytometry Facility.
Isolation of MNCs is a separate charge.
Price on request.
Retrospective analysis of FCS3.0 files:
Detailed/retrospective flow cytometry analysis utilizing FloJo
-Data mining/analysis/re-analysis of previously acquired list mode data (FCS3.0 files) of EPC or CHSPC phenotyping for newly identified cell sub-populations of interest.
$100.00 USD per Hour
Protocol design and development:
Project specific consulting for investigators interested in the implementation of flow cytometry utilizing EPCs and circulating hematopoietic stem and progenitor cells (CHSPCs) as biomarkers in pre-clinical and clinical phase applications.
$100.00 USD per Hour
Deliverables:
Turnaround time: ~2 weeks
Plasma Separation and Freeze Down:
Preparation includes centrifuging and freeze down of up to 8 vials of plasma.
$12 USD per Sample
MNC Separation and Freeze Down:
Preparation includes isolation of MNCs and freeze down of up to 5 vials of MNCs
$82.00 USD per Sample
Plating, culture and quantitation of CFU-GEMMs (in triplicate)
Isolation of MNCs is a separate charge
Turnaround time: ~2 weeks
"Great communication. Pleasure to work with again."
"Great experience. Responsive. THX"
Angio BioCore (ABC) has not received any endorsements.