For more than 24 years, our team of dedicated scientists have provided high-quality gDNA BAC Libraries, DNA library screening, HMW gDNA preparation, and molecular biology cloning services to researchers around the world. We provide high-quality products and services at competitive prices.
Amplicon Express’ team of dedicated scientists have made over 2,650 custom BAC libraries and processed more than 57 million high-quality, large insert genomic DNA clones since 1996. We are committed to providing the highest quality BAC library resource on the market in a timely and cost-effective manner.
Our extensive experience extracting and cloning HMW gDNA fragments from thousands of different organisms, has enabled us to optimize DNA extraction, purification and ligation protocols to be successful where others have failed. We love working with marine organisms (fish, invertebrates, sponges, etc), plants with strong secondary/defense compounds, troublesome insects and other difficult critters!
BAC Libraries are an ideal tool for Metagenomic Libraries (each +100Kb insert is from a different organism).
Amplicon Express Custom BAC libraries have average insert sizes between 115 Kb and 180 Kb and are used by thousands of researchers in more than 65 countries around the world.
We pride ourselves on delivering individualized service and project design from the beginning of the quoting process to our one-on-one technical support years after the library has reached its destination.
Amplicon Express has made hundreds of Fosmid libraries from various organisms including metagenomic soil and deep seawater samples. Whether you need one library or many, arrayed clones or titered transfections our team of scientists will construct the fosmid library you need. We pride ourselves on individual service and project design, as well as technical guidance for using this powerful resource.
Additionally we can prepare NGS grade DNA and/or ready to run illumina NGS libraries from individual Fosmid clones, pools of Fosmid clones or unpicked pools of titered transfected clones.
We make DNA for maximum read lengths on PacBio RSII optimized for +20Kb SMRTcell protocol. Click to share details about your project and get a quote
See details on 18 Kb average first strand read length, using our NGS grade DNA (in collaboration with Pacific Biosciences R&D team).
Amplicon Express offers a variety of DNA isolation services and protocols to suit all your needs. Cost effective and highly optimized methods for obtaining High Quality DNA for all types of organisms and applications.
- High molecular weight (HMW) DNA Isolations for Megabase Sized DNA optimized for Optical Mapping or (BioNano IRYS platform).
- Modified CTAB extraction from plants: +20µg of ultra clean Next Generation Sequencing (NGS) grade DNA per extraction.
- Specializing in Metagenomic DNA extraction (i.e. soil, filtered marine water, hot spring, marine sponge communities).
- 96-well DNA extraction from BAC and Fosmid clones in ng to µg amounts suitable for all NGS applications.
- Genomic DNA extraction: suitable for all applications (NGS, PCR, DIGEST).
“Screening a genomic DNA BAC Library” is identifying a Region Of Interest (ROI) on a BAC clone insert or Fosmid clone insert. Researchers can then study the entire gene region surround the ROI, promoters, 5′ and 3′ UTR and tgenic inserts. Amplicon Express specializes in library screening services. You simply provide any known information, we design and optimize probes, build screening tools (Nylon Filters or Pools & Superpools) and screen the resource for you. We can screen a pre-existing genomic DNA libraries (BAC, PAC, TAC, Fosmid, Cosmid etc), or create a custom gDNA library for your project.
WGP is a powerful method for Whole Genome Physical Map construction developed in partnership with KeyGene. WGP is a powerful strategic technique we have applied to dozens of plant genome projects since announcing the technology at the 2009 Plant & Animal Genome Conference.
The following improvements have been made to the original WGP technology
- N50 contigs: From 560kb to 3.3Mb
- Number of contigs dramatically decreased
- Removal > 90% of problematic BACs
- Higher fraction of BACs placed in contigs with fewer singletons remaining
- Improved physical map (FPC assembly)
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