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Amid Biosciences LLC

11 Orders Completed
Fremont, California, US

About Amid Biosciences LLC

Amid Biosciences develops and manufactures products to simplify and improve life science research. Our mission is to help biomedical researchers save time and cost to accomplish their research needs.

Amid Biosciences was founded by scientists with extensive research experience at academic institutions and... Show more »

Amid Biosciences develops and manufactures products to simplify and improve life science research. Our mission is to help biomedical researchers save time and cost to accomplish their research needs.

Amid Biosciences was founded by scientists with extensive research experience at academic institutions and industry. Some of our past research have been published in journals such as Science, Molecular Cell, Genes & Development, Angewandte Chemie, and Journal of the American Chemical Society.

Our core competencies include enzyme development (DNA and RNA polymerases), protein expression and purification with emphasis on bacterial systems, and cloning reagents. We specifically focus on protein expression tools for academic, industrial and government entities.

We take pride in offering high quality products at competitive prices. We perform quality control on each batch of products to ensure that they meet industry performance specifications.

Selected Publications

  • Toulokhonov, I., et al. Allosteric control of RNA polymerase by a site that contacts nascent RNA hairpins. Science. 2001 Apr 27;292(5517):730-3.
  • Toulokhonov, I., et al. A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Mol Cell. 2007 Aug 3;27(3):406-19.
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Diversity Certificates

Minority owned business Small business

Our Services (27)


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Protein Purification

Price on request

Amid Biosciences’ team uses industry standard diverse chromatographic methods to purify recombinant proteins.

We employ known protocols or perform process development when the protocols are not available.

Some of our most popular protein purification approaches are:

• Affinity chromatography (his tag, GST, Strep, MBP,... Show more »

Amid Biosciences’ team uses industry standard diverse chromatographic methods to purify recombinant proteins.

We employ known protocols or perform process development when the protocols are not available.

Some of our most popular protein purification approaches are:

• Affinity chromatography (his tag, GST, Strep, MBP, Protein A/G/L)
• Ion-exchange chromatography
• Gel filtration / Size-exclusion chromatography

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Beckman Coulter Inc. Avanti Floor Centrifuge J-25 AKTA FPLC Affinity chromatography gel filtration chromatography ion exchange chromatography Yeast Bacteria Pichia pastoris E. coli Show 9 more tags Show less

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Protein Expression Screening and Optimization

Price on request

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at... Show more »

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project. Expression services can be requested independently or in conjunction with our purification services.

Bacterial expression

Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli is one of the most widely used hosts for the expression of recombinant proteins. Amid Biosciences offers recombinant protein expression in a variety of commercially available E. coli expression strains. Amid Biosciences has the capacity to quickly screen soluble expression conditions for multiple expression constructs.

Our service offerings include:

  • Gene cloning into proprietary or commercial expression vectors for customized protein expression and
    purification.
  • Expression optimization with inducer, temperature and time course.
  • Growth monitoring for consistency batch to batch.
  • Rapid scale up to 10 L in shake flasks.
  • Routinely increased total or soluble protein expression by more than 10-fold using proprietary techniques.
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Yeast Bacteria E. coli

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Protein Expression in Yeast

Price on request

Amid Biosciences offers recombinant protein expression in yeast ( Saccharomyces cerevisiae, Pichia pastoris).

Amid Biosciences offers recombinant protein expression in yeast ( Saccharomyces cerevisiae, Pichia pastoris).

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Pichia pastoris Saccharomyces cerevisiae

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Custom Competent Cell Production

Price on request

Competent cells are necessary tools for molecular cloning, construction of DNA libraries, recombinant protein expression, and mutagenesis in every molecular biology laboratory.
Currently, Amid Biosciences offers AB 5-alpha, BL21(DE3), DH10B, phage display TG1 competent cells at economical prices. Both high efficiency... Show more »

Competent cells are necessary tools for molecular cloning, construction of DNA libraries, recombinant protein expression, and mutagenesis in every molecular biology laboratory.
Currently, Amid Biosciences offers AB 5-alpha, BL21(DE3), DH10B, phage display TG1 competent cells at economical prices. Both high efficiency chemically competent and electrocompetent cells are available.
Average 2-3 week turn-around.
Guaranteed performance.

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Recombinant Protein Expression

Price on request

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at... Show more »

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project. Expression services can be requested independently or in conjunction with our purification services.

Bacterial expression

Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli is one of the most widely used hosts for the expression of recombinant proteins. Amid Biosciences offers recombinant protein expression in a variety of commercially available E. coli expression strains. Amid Biosciences has the capacity to quickly screen soluble expression conditions for multiple expression constructs.

Our service offerings include:

  • Gene cloning into proprietary or commercial expression vectors for customized protein expression and purification.
  • Expression optimization with inducer, temperature and time course.
  • Growth monitoring for consistency batch to batch.
  • Rapid scale up to 10 L in shake flasks.
  • Routinely increased total or soluble protein expression by more than 10-fold using proprietary techniques.
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AKTA FPLC TEV Protease Affinity chromatography SDS-PAGE Toxic Protein Expression Protein Solubility Enhancement Tight Control non-T7 promoters bacterial based recombinant protein production gel filtration chromatography Yeast Bacteria E. coli Show 13 more tags Show less

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Bacterial Protein Expression

Price on request

Amid Biosciences offers protein expression and production in E. coli and Bacillus subtilis. Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli... Show more »

Amid Biosciences offers protein expression and production in E. coli and Bacillus subtilis. Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli and Bacillus subtillis are the most widely used hosts for the expression and production of recombinant proteins. Amid Biosciences offers recombinant protein expression in a variety of commercially available E. coli expression strains. Amid Biosciences has the capacity to quickly screen soluble expression conditions for multiple expression constructs. Our service offerings include: - Gene cloning into proprietary or commercial expression vectors for customized protein expression and purification. - Expression optimization with inducer, temperature and time course. - Growth monitoring for consistency batch to batch. - Rapid scale up to 10 L in shake flasks. - Routinely increased total or soluble protein expression by more than 10-fold using proprietary techniques.

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AKTA FPLC Bacillus subtillis Affinity chromatography E. coli

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Protein Services

Price on request

AMID BIOSCIENCES provides protein services from gene synthesis to protein expression, refolding and purification.

Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of... Show more »

AMID BIOSCIENCES provides protein services from gene synthesis to protein expression, refolding and purification.

Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project.

Expression services can be requested independently or in conjunction with Amid Biosciences purification services.

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E. coli

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Protein Refolding

Price on request
Request a quote for more information about this service.
E. coli

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Protein Production

Price on request

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at... Show more »

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project. Expression services can be requested independently or in conjunction with our purification services.

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Biology Bacteria E. coli

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Competent Cell Production

Price on request

Competent cells are necessary tools for molecular cloning, construction of DNA libraries, recombinant protein expression, and mutagenesis in every molecular biology laboratory.
Currently, Amid Biosciences offers AB 5-alpha, BL21(DE3), DH10B, phage display TG1 competent cells at economical prices. Both high efficiency chemically competent and electrocompetent cells are available.

Competent cells are necessary tools for molecular cloning, construction of DNA libraries, recombinant protein expression, and mutagenesis in every molecular biology laboratory.
Currently, Amid Biosciences offers AB 5-alpha, BL21(DE3), DH10B, phage display TG1 competent cells at economical prices. Both high efficiency chemically competent and electrocompetent cells are available.

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Molecular Cloning

Price on request

Amid Biosciences provides a high-quality and user-friendly Molecular Cloning service. Since our expression vectors are optimized for high level expression, the gene of interest can be cloned into one of our vectors and may include tag fusion for rapid purification and assay detection. Removal of the tagging can be performed by... Show more »

Amid Biosciences provides a high-quality and user-friendly Molecular Cloning service. Since our expression vectors are optimized for high level expression, the gene of interest can be cloned into one of our vectors and may include tag fusion for rapid purification and assay detection. Removal of the tagging can be performed by cleavage with a specific protease.

The basic procedure for gene cloning:

  • Amplify the gene of interest from a cDNA library, genomic DNA, cell line, hybridoma or a BAC clone by PCR.
  • Clone the PCR product into a cloning vector.
  • Sub-clone the gene into our expression vector.
  • The cloned gene will be sequenced in both orientations to ensure the correct frameshift.
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Custom Gene Cloning

Price on request

Amid Biosciences provides a high-quality and user-friendly Gene Cloning service. Since our expression vectors are optimized for high level expression, the gene of interest can be cloned into one of our vectors and may include tag fusion for rapid purification and assay detection. Removal of the tagging can be performed by cleavage... Show more »

Amid Biosciences provides a high-quality and user-friendly Gene Cloning service. Since our expression vectors are optimized for high level expression, the gene of interest can be cloned into one of our vectors and may include tag fusion for rapid purification and assay detection. Removal of the tagging can be performed by cleavage with a specific protease.

The basic procedure for gene cloning:

  • Amplify the gene of interest from a cDNA library, genomic DNA, cell line, hybridoma or a BAC clone by PCR.
  • Clone the PCR product into a cloning vector.
  • Sub-clone the gene into our expression vector.
  • The cloned gene will be sequenced in both orientations to ensure the correct frameshift.
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DNA Ligase from T4-infected Escherichia coli Restriction Enzymes High-Fidelity DNA polymerase PCR Cloning Ligation Independent Cloning Seamless Cloning Gibson Cloning Restriction Enzymes Based Cloning Show 9 more tags Show less

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Assay Development

Price on request

Custom Assay Development for Nucleic Acid Amplification Enzymes

Custom Assay Development for Nucleic Acid Amplification Enzymes

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PCR thermal cycler DNA polymerase RNA polymerase Infectious Disease PCR Colorimetric Fluorescence isothermal amplification LAMP Show 9 more tags Show less

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Bioconjugation/Chemical Crosslinking

Price on request

Protein labeling and modifications include conjugation of fluorescent dyes, crosslinking moieties, linkers, biotinylation and etc. Chemical linking of various molecules to agarose beads or surfaces (plates, glass slides) for affinity chromatography or ELISA. Common conjugation procedures include coupling of proteins, peptides,... Show more »

Protein labeling and modifications include conjugation of fluorescent dyes, crosslinking moieties, linkers, biotinylation and etc. Chemical linking of various molecules to agarose beads or surfaces (plates, glass slides) for affinity chromatography or ELISA. Common conjugation procedures include coupling of proteins, peptides, antibodies, small molecules, such as biotin or fluorophores, oligosaccharides, nucleic acids to agarose beads and other surfaces.

Fluorophore Labeling

1) Protein labeling with commercially available dyes derivatized with Succinimidyl-Esters,        Maleimides or other crosslinking mojeties
2) Excess dye removal using dialysis
3) Buffer exchange into PBS; all other formulations may require an additional fee per sample
4) Fluorophore to Protein ratios (F/P) determination using spectrophotometry

Biotinylation:

In vitro biotinylation via BirA biotin ligase: 
1) Buffer exchange Avi-tagged protein into biotinylation compatible buffer

2) Addition of BirA enzyme at appropriate ratios for biotinylation

3) Dialysis to remove free biotin 

4) Affinity purification to remove BirA

In vivo biotinylation via BirA:
1) Co-expression of BirA with Avi-tagged protein of interest in expression host system

2) Addition of D-biotin to expression culture

3) Purification of Avi-tagged protein 

 

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Biotin fluorophores crosslinking reagents affinity tag linkers non-natural amino acids Biotinylation crosslinking chemical modification fusion protein quantification Show 11 more tags Show less

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Site-Directed Mutagenesis

Price on request
Request a quote for more information about this service.
Point Mutations Amino Acid Substitution Deletions Insertions

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Antibody Services

Price on request
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Nucleic Acid Services

Price on request
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DNA Services

Price on request
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DNA Extraction and Purification

Price on request
Request a quote for more information about this service.

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Mutagenesis

Price on request
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Antibody Modification

Price on request
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Antibody Purification

Price on request
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Biochemistry & Molecular Biology

Price on request
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Project Management

Price on request
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Biology

Price on request
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Synthetic Chemistry

Price on request
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Chemistry and Materials

Price on request
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Innokenti Toulokhonov

Founder/ Scientist
  • Positive review for Bacterial Protein Expression:

    July 2017

    "I was having trouble performing a DE3 lysogenization into a bacterial strain that lacked T7 RNA polymerase for protein production. I asked Science Exchange for help in finding someone who could do the work. They sent me several options and I went with Amid Biosciences because they had the most simple proposal, it was inexpensive, and quoted the quickest turnaround out of any of the other companies. Without going into all of the details Amid was very reasonable priced, delivered on time, were extremely communicative on the progress of the work, and wrote up a extremely detailed report once the work was finished. I would highly recommend working with Amid Biosciences for any of you bacterial/protein expression needs. Bridge Hunter- Sanofi-Genzyme"

Amid Biosciences LLC has not received any endorsements.