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Amid Biosciences LLC

Fremont, California, US

Amid Biosciences develops and manufactures products to simplify and improve life science research. Our mission is to help biomedical researchers save time and cost to accomplish their research needs.

Amid Biosciences was founded by scientists with extensive research experience at academic institutions and industry. Some of our past research have been published in journals such as Science, Molecular Cell, Genes & Development, Angewandte Chemie, and Journal of the American Chemical Society.

Our core competencies include enzyme development (DNA and RNA polymerases), protein expression and purification with emphasis on bacterial systems, and cloning reagents. We specifically focus on protein expression tools for academic, industrial and government entities.

We take pride in offering high quality products at competitive prices. We perform quality control on each batch of products to ensure that they meet... Show more »

Amid Biosciences develops and manufactures products to simplify and improve life science research. Our mission is to help biomedical researchers save time and cost to accomplish their research needs.

Amid Biosciences was founded by scientists with extensive research experience at academic institutions and industry. Some of our past research have been published in journals such as Science, Molecular Cell, Genes & Development, Angewandte Chemie, and Journal of the American Chemical Society.

Our core competencies include enzyme development (DNA and RNA polymerases), protein expression and purification with emphasis on bacterial systems, and cloning reagents. We specifically focus on protein expression tools for academic, industrial and government entities.

We take pride in offering high quality products at competitive prices. We perform quality control on each batch of products to ensure that they meet industry performance specifications.

Selected Publications

  • Toulokhonov, I., et al. Allosteric control of RNA polymerase by a site that contacts nascent RNA hairpins. Science. 2001 Apr 27;292(5517):730-3.
  • Toulokhonov, I., et al. A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Mol Cell. 2007 Aug 3;27(3):406-19.
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Amid Biosciences LLC has not listed any services.

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Protein Purification
Price on request

Amid Biosciences’ team uses industry standard diverse chromatographic methods to purify recombinant proteins.

We employ known protocols or perform process development when the protocols are not available.

Some of our most popular protein purification approaches are:

• Affinity chromatography (his tag, GST, Strep, MBP,... Show more »

Amid Biosciences’ team uses industry standard diverse chromatographic methods to purify recombinant proteins.

We employ known protocols or perform process development when the protocols are not available.

Some of our most popular protein purification approaches are:

• Affinity chromatography (his tag, GST, Strep, MBP, Protein A/G/L)
• Ion-exchange chromatography
• Gel filtration / Size-exclusion chromatography

« Show less
Beckman Coulter Inc. Avanti Floor Centrifuge J-25
Yeast
E. coli
Bacteria
Pichia pastoris
AKTA FPLC
Affinity chromotography
Affinity Tag Cleavage
ion-exchange chromotography
gel filtration chromotography
bacterial based protein expression
Protein Expression Optimization
Price on request

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at... Show more »

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project. Expression services can be requested independently or in conjunction with our purification services.

Bacterial expression

Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli is one of the most widely used hosts for the expression of recombinant proteins. Amid Biosciences offers recombinant protein expression in a variety of commercially available E. coli expression strains. Amid Biosciences has the capacity to quickly screen soluble expression conditions for multiple expression constructs.

Our service offerings include:

  • Gene cloning into proprietary or commercial expression vectors for customized protein expression and
    purification.
  • Expression optimization with inducer, temperature and time course.
  • Growth monitoring for consistency batch to batch.
  • Rapid scale up to 10 L in shake flasks.
  • Routinely increased total or soluble protein expression by more than 10-fold using proprietary techniques.
« Show less
Yeast
E. coli
Bacteria
Custom Competent Cell Production
Price on request

Competent cells are necessary tools for molecular cloning, construction of DNA libraries, recombinant protein expression, and mutagenesis in every molecular biology laboratory.
Currently, Amid Biosciences offers AB 5-alpha, BL21(DE3), DH10B, phage display TG1 competent cells at economical prices. Both high efficiency... Show more »

Competent cells are necessary tools for molecular cloning, construction of DNA libraries, recombinant protein expression, and mutagenesis in every molecular biology laboratory.
Currently, Amid Biosciences offers AB 5-alpha, BL21(DE3), DH10B, phage display TG1 competent cells at economical prices. Both high efficiency chemically competent and electrocompetent cells are available.
Average 2-3 week turn-around.
Guaranteed performance.

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Recombinant Protein Expression
Price on request

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at... Show more »

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project. Expression services can be requested independently or in conjunction with our purification services.

Bacterial expression

Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli is one of the most widely used hosts for the expression of recombinant proteins. Amid Biosciences offers recombinant protein expression in a variety of commercially available E. coli expression strains. Amid Biosciences has the capacity to quickly screen soluble expression conditions for multiple expression constructs.

Our service offerings include:

  • Gene cloning into proprietary or commercial expression vectors for customized protein expression and purification.
  • Expression optimization with inducer, temperature and time course.
  • Growth monitoring for consistency batch to batch.
  • Rapid scale up to 10 L in shake flasks.
  • Routinely increased total or soluble protein expression by more than 10-fold using proprietary techniques.
« Show less
TEV Protease
Yeast
E. coli
Bacteria
Affinity purification
AKTA FPLC
Affinity chromotography
SDS-PAGE
Recombinant protein production
Expression optimization
ion-exchange chromatography
gel-filtration
Toxic Protein Expression
Protein Solubility Enhancement
Affinity Tag Cleavage
Tight Control
non-T7 promoters
Protein Refolding
bacterial based recombinant protein production
Bacterial Protein Expression
Price on request

Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli is one of the most widely used hosts for the expression of recombinant proteins. Amid... Show more »

Bacterial expression is a rapid and economical system for high level production of recombinant proteins. Amid Biosciences offers custom bacterial expression, providing consultation and optimization of services for each project. E. coli is one of the most widely used hosts for the expression of recombinant proteins. Amid Biosciences offers recombinant protein expression in a variety of commercially available E. coli expression strains. Amid Biosciences has the capacity to quickly screen soluble expression conditions for multiple expression constructs.

Our service offerings include:

  • Gene cloning into proprietary or commercial expression vectors for customized protein expression and
    purification.
  • Expression optimization with inducer, temperature and time course.
  • Growth monitoring for consistency batch to batch.
  • Rapid scale up to 10 L in shake flasks.
  • Routinely increased total or soluble protein expression by more than 10-fold using proprietary techniques.
« Show less
E. coli
AKTA FPLC
Affinity chromotography
Affinity Tag Cleavage
Protein Services
Price on request

AMID BIOSCIENCES provides protein services from gene synthesis to protein expression, refolding and purification.

Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of... Show more »

AMID BIOSCIENCES provides protein services from gene synthesis to protein expression, refolding and purification.

Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project.

Expression services can be requested independently or in conjunction with Amid Biosciences purification services.

« Show less
E. coli
Bacteria
Protein Refolding
Price on request
Request a quote for more information about this service.
E. coli
Protein Expression
Price on request

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at... Show more »

Amid Biosciences offers comprehensive support in protein expression and purification for the biotech industry -- starting a project where YOU want to start. Amid Biosciences has a team of experienced and highly skilled protein expression scientists. We offer recombinant protein expression in E. coli. Projects can be initiated at any point from sequence information through delivery of transformation-ready expression plasmid. Our knowledgeable staff will provide consultation services to aid in the development of milestone driven quotations that ensure client satisfaction during the project. Clients are provided real-time project updates to guide the project’s progression and receive presentation quality technical reports at the conclusion of the project. Expression services can be requested independently or in conjunction with our purification services.

« Show less
E. coli
Bacteria
Biology
bacterial based protein expression
Mixture
Competent Cell Production
Price on request

Competent cells are necessary tools for molecular cloning, construction of DNA libraries, recombinant protein expression, and mutagenesis in every molecular biology laboratory.
Currently, Amid Biosciences offers AB 5-alpha, BL21(DE3), DH10B, phage display TG1 competent cells at economical prices. Both high efficiency chemically competent and electrocompetent cells are available.

Competent cells are necessary tools for molecular cloning, construction of DNA libraries, recombinant protein expression, and mutagenesis in every molecular biology laboratory.
Currently, Amid Biosciences offers AB 5-alpha, BL21(DE3), DH10B, phage display TG1 competent cells at economical prices. Both high efficiency chemically competent and electrocompetent cells are available.

« Show less
Molecular Cloning
Price on request

Amid Biosciences provides a high-quality and user-friendly Molecular Cloning service. Since our expression vectors are optimized for high level expression, the gene of interest can be cloned into one of our vectors and may include tag fusion for rapid purification and assay detection. Removal of the tagging can be performed by... Show more »

Amid Biosciences provides a high-quality and user-friendly Molecular Cloning service. Since our expression vectors are optimized for high level expression, the gene of interest can be cloned into one of our vectors and may include tag fusion for rapid purification and assay detection. Removal of the tagging can be performed by cleavage with a specific protease.

The basic procedure for gene cloning:

  • Amplify the gene of interest from a cDNA library, genomic DNA, cell line, hybridoma or a BAC clone by PCR.
  • Clone the PCR product into a cloning vector.
  • Sub-clone the gene into our expression vector.
  • The cloned gene will be sequenced in both orientations to ensure the correct frameshift.
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Custom Gene Cloning
Price on request

Amid Biosciences provides a high-quality and user-friendly Gene Cloning service. Since our expression vectors are optimized for high level expression, the gene of interest can be cloned into one of our vectors and may include tag fusion for rapid purification and assay detection. Removal of the tagging can be performed by cleavage... Show more »

Amid Biosciences provides a high-quality and user-friendly Gene Cloning service. Since our expression vectors are optimized for high level expression, the gene of interest can be cloned into one of our vectors and may include tag fusion for rapid purification and assay detection. Removal of the tagging can be performed by cleavage with a specific protease.

The basic procedure for gene cloning:

  • Amplify the gene of interest from a cDNA library, genomic DNA, cell line, hybridoma or a BAC clone by PCR.
  • Clone the PCR product into a cloning vector.
  • Sub-clone the gene into our expression vector.
  • The cloned gene will be sequenced in both orientations to ensure the correct frameshift.
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DNA Ligase from T4-infected Escherichia coli
PCR
Cloning
Ligation Independent Cloning
Seamless Cloning
Gibson Cloning
Restriction Enzymes Based Cloning
Restriction Enzymes
High-Fidelity DNA polymerase
Assay Development
Price on request

Custom Assay Development for Nucleic Acid Amplification Enzymes

Custom Assay Development for Nucleic Acid Amplification Enzymes

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PCR
Colorimetric
Fluorescence
bacterial infections
viruses
PCR thermocycler
isothermal amplification
LAMP
DNA polymerase
RNA polymerase
Chemical Crosslinking/Bioconjugation
Price on request

Protein labeling and modifications include conjugation of fluorescent dyes, crosslinking moieties, linkers, biotinylation and etc.

Chemical linking of various molecules to agarose beads or surfaces (plates, glass slides) for affinity chromatography or ELISA. Common conjugation procedures include coupling of proteins, peptides,... Show more »

Protein labeling and modifications include conjugation of fluorescent dyes, crosslinking moieties, linkers, biotinylation and etc.

Chemical linking of various molecules to agarose beads or surfaces (plates, glass slides) for affinity chromatography or ELISA. Common conjugation procedures include coupling of proteins, peptides, antibodies, small molecules, such as biotin or fluorophores, oligosaccharides, nucleic acids to agarose beads and other surfaces.

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Biotin
Biotinylation
crosslinking
chemical modification
fusion
protein quantification
fluorophores
crosslinking reagents
affinity tag
linkers
non-natural amino acids
Site-Directed Mutagenesis
Price on request
Request a quote for more information about this service.
Point Mutations
Amino Acid Substitution
Deletions
Insertions
Protein Purification and Quantification
Price on request

Amid Biosciences’ team uses industry standard diverse chromatographic methods to purify recombinant proteins.

We employ known protocols or perform process development when the protocols are not available.

Some of our most popular protein purification approaches are:

• Affinity chromatography (his tag, GST, Strep, MBP,... Show more »

Amid Biosciences’ team uses industry standard diverse chromatographic methods to purify recombinant proteins.

We employ known protocols or perform process development when the protocols are not available.

Some of our most popular protein purification approaches are:

• Affinity chromatography (his tag, GST, Strep, MBP, Protein A/G/L)
• Ion-exchange chromatography
• Gel filtration / Size-exclusion chromatography

« Show less
Antibody Services
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.
DNA Services
Price on request
Request a quote for more information about this service.
DNA Extraction and Purification
Price on request
Request a quote for more information about this service.
Mutagenesis
Price on request
Request a quote for more information about this service.
Protein Conjugation
Price on request
Request a quote for more information about this service.
Antibody Modification
Price on request
Request a quote for more information about this service.
Antibody Purification
Price on request
Request a quote for more information about this service.
Antibody Conjugation
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Experimental Design
Price on request
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Project and Process Management
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.
Synthetic Chemistry
Price on request
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Chemistry and Materials
Price on request
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2018-05-29 18:31:54 -0400

Net Promoter Score of 10 received for Custom Competent Cell Production.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-12-10 06:56:42 -0500

Net Promoter Score of 10 received for Site-Directed Mutagenesis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-12-05 16:50:57 -0500

Net Promoter Score of 9 received for Bacterial Protein Expression.

2017-12-05 16:50:28 -0500

Net Promoter Score of 9 received for Bacterial Protein Expression.

July 14, 2017

Positive review received for Bacterial Protein Expression:

"I was having trouble performing a DE3 lysogenization into a bacterial strain that lacked T7 RNA polymerase for protein production. I asked Science Exchange for help in finding someone who could do the work. They sent me several options and I went with Amid Biosciences because they had the most simple proposal, it was inexpensive, and quoted the quickest turnaround out of any of the other companies. Without going into all of the details Amid was very reasonable priced, delivered on time, were extremely communicative on the progress of the work, and wrote up a extremely detailed report once the work was finished. I would highly recommend working with Amid Biosciences for any of you bacterial/protein expression needs. Bridge Hunter- Sanofi-Genzyme"

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