Each nanoparticle formulation is unique, and each instrument or technique has it's optimal range and certain limitations. We chose particular technique for each nanoparticle sample taking into account what is the nature of that sample and what information that method can give us, and perform rigorous method validation and optional cross-validation using other techniques.
Nanoparticle Tracking Analysis Nanosight
Nanosight LM10-HS (Malvern) provides accurate nanoparticles size and concentration measurement in the range 30-1000nm. Instrument requires only 0.5 mL of the sample with concentration range 10^7 to 10^9 particles/mL. This technique is most widely used in exosome analysis. Unlike DLS (dynamic light scattering) Nanosight is capable of resolving sub-populations of particles with close size. Turn around for orders under 20 samples - 3-5 business days.
Laser diffraction analysis of size and size distribution of the particles in solution in the range 100nm-900um.
Potentiometric titration for determination of water content in solutions and dry powders.
By Zeta-sizer Nano ZS (Malvern) to evaluate particles size and size distribution
200Kv transmission electron microscopy to cross-validate other techniques or aquire images
Zetasizer Nano ZS (Malvern) requires minimum of 0.5 mL of sample in electrolyte (PBS, NaCl solutions fit), analysis turnaround time - 5 business days. Instrument report will be provided.
Method development, qualification and validation. Method development to include LOD, LOQ, Linearity, Accuracy, Precision.
From 1 nm to 1000 um: we use laser diffraction (X-100 by Microtrac and Spraytec by Malvern) to analyze dry powder particels, nasal sprays, aerosols, inhalers and solutions of microparticles and DLS (Zetasizer Nano ZS by Malvern), NTA (Nanosight by Malvern), TRPS (qNano by Izon) for analysis of nanoparticles. Electron microscope by TEM/SEM is offered to confirm achieved data.
Exosomes and extracellular vesicles (EV) play important roles in myriad of biological processes and are widely studied. Biological matrix, isolation and purification steps, heterogeneity of particles’ size make characterization of exosomes considerably complex for most techniques available on the market. Over the past few years Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) technology by Izon were found to be the most suitable and reliable techniques for analysis of variety of nanoparticles, and found to be exceptionally suitable for exosomes characterization.
NTA sinultaneously identifies and tracks each individual particle moving under Brownian motion and relates movement trajectory to particle size. It results in exceptional capability to resolve and count particles of different size in the mixture.
TRPS is an impedance-based system and is the only technique that can deliver three fundamental parameters of nanoparticles:
A voltage is applied across a pore that is filled with electrolyte, resulting in an ionic current. As particles cross the pore they briefly increase electrical resistance, creating a resistive pulse, which is precisely proportional to particle volume. TRPS represents a rapid, convenient, accurate and reproducible method for nanoparticle analysis.
Both Nanosight and qNano are excellent complimentary tools to study and characterize exosomes. AlphaNanoTech operates with Nanosight NS200-HS (Malvern) and qNano (IZON). Cross-validation by Dynamic Light Scattering (DLS) and SEM/TEM imaging is available upon request.
AlphaNanoTech provides top level nanoparticle characterization services with following optional cross-validation by Tunable Resistive Pulse Sensing (TRPS) qNano technology by Izon, Nanoparticle tracking analysis by Malvern Nanosight, and SEM/TEM imaging.
Exosomes and Extracellular vesicles
Exosomes and microvesicles play important role in transmission of biological signals between cells. Pathophysiological role of exosomes is beginning to be realized in cancer, neurodegenerative diseases, infectious diseases and other conditions. They are also attracting significant interest as drug delivery and transfection vehicles.
Nanoparticles as drug delivery systems gained a lot of attention in past few decades. There is number of FDA approved nanomedicines on the market for treatment of cancer, infectious diseases, cardiac/vascular disorders, viral infections, and others. Nanoparticles are also used in vaccines, cosmetics, for imaging nad theranostics.
Aggregation state of viral particles or adjuvant, as well as their count is crucial to understand and optimize vaccine's biological activity and evaluate long term stability.
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