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Aldevron - Protein

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Madison, Wisconsin, US

About Aldevron - Protein

Divison of: Aldevron

Aldevron is global leader in biological products and contract manufacturing services, specializing in plasmid DNA manufacturing, protein services and antibody development.

Our protein production capabilities range from parallel screening of several constructs through process development optimization, to... Show more »

Aldevron is global leader in biological products and contract manufacturing services, specializing in plasmid DNA manufacturing, protein services and antibody development.

Our protein production capabilities range from parallel screening of several constructs through process development optimization, to "scale-up" manufacturing. With experience producing thousands of proteins, we identify potential risks and engineer a solution to meet your required specifications. Responsiveness, technical excellence, and strong project management form the foundation of every strong partnership. It’s our goal to embody those qualities and earn your trust every step of the way.

We develop strategies in E. coli, insect and mammalian expression systems to produce proteins specific for your application. Our team can execute both technical transfer of your protocol or develop a customized expression and purification process.

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Our Services (30)


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Cas9 Nuclease

Price on request

Our Cas9 nucleases are highly concentrated, low in endotoxin, and extremely (>95%) pure. These enzymes are manufactured to rigid quality specifications, providing consistent and scalable results from research through preclinical and clinical applications. Aldevron Cas9 nucleases are designed for multiple delivery methods,... Show more »

Our Cas9 nucleases are highly concentrated, low in endotoxin, and extremely (>95%) pure. These enzymes are manufactured to rigid quality specifications, providing consistent and scalable results from research through preclinical and clinical applications. Aldevron Cas9 nucleases are designed for multiple delivery methods, including electroporation, transfection, microinjection, and whole animal applications.

These are the nucleases available from Aldevron:
(Pricing as of November 2018.)

SpyFi™ Nuclease Cas9 (9214-0.25MG) - $600.00 - 0.25 mg
SpyFI Cas9 Nuclease (9214-5MG) - $8980.00 - 5 mg
sNLS-SpCas9-sNLS Nuclease (9212-0.25MG) - $365.00 - 0.25mg
sNLS-SpCas9-sNLS Nuclease (9212-5MG) - $6250.00 – 5mg
His-sNLS-myc-SpCas9-HA-nucNLS Nuclease (9204-0.25MG) - $365.00 – 0.25mg
His-sNLS-myc-SpCas9-HA-nucNLS Nuclease (9204-5MG) - $6250.00 – 5mg
sNLS-AsCpf1-sNLS Nuclease (9213-0.25MG) - $365.00 – 0.25mg
sNLS-AsCpf1-sNLS Nuclease (9213-0.5MG) - $720.00 – 0.50mg
sNLS-AsCpf1-sNLS Nuclease (9213-5MG) - $6850.00 – 5mg

Need to order in bulk?
If you'd like to order Cas9 products in bulk, or need more information about our Cas9 products, please contact us.

In addition to the existing formats, Aldevron Cas9 nucleases can be customized to suit your specific needs. As your custom development and manufacturing partner for Cas9 and related nucleases, we offer a range of options:
• Process development and production starting from a gene sequence.
• Process development and production starting with your expression plasmid or cell paste.
• Custom production starting from your manufacturing processes.

Key Features:
• 95% Purity, Low Endotoxin,
• High Concentration – 10 mg/mL
• Variety of NLS locations, His and/or Myc tags
• Available in bulk – mg to g scale

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Oncology Genetic Disease Genetic myopathies Cas9 Crispr System Cas9 Crispr Cas9 Knockout CRISPR Cas9 CRISPR Cas9 Gene Editing Technology gram milligram Show 10 more tags Show less

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Bacterial Protein Expression

Starting at $1,100.00 per unit

Aldevron provides screening, expression, and purification services to isolate your target protein. All common expression strains of E. coli are available; we will work with you to select the optimal strain for each project.

Bacterial Expression Screening: (Starting at $2556 per target)
• Test recombinant protein expression in... Show more »

Aldevron provides screening, expression, and purification services to isolate your target protein. All common expression strains of E. coli are available; we will work with you to select the optimal strain for each project.

Bacterial Expression Screening: (Starting at $2556 per target)
• Test recombinant protein expression in one or two strains under six (6) conditions.
• Transform pDNA into bacterial cells, incubate, harvest cell paste.
• Analyze soluble and insoluble fractions by SDS-PAGE.

Bacterial Expression Scale-up:
• 1-240 L in shake flasks
• 5-440 L batch fermentation
• Use optimized conditions from expression screen, or standard methods.
• Transform E. coli, prepare culture, induce expression, harvest cell paste.
• Report results, deliver cell paste OR use paste as starting material for purification

Standard Quality Control:
• SDS PAGE
• A280
• RP-HPLC
• Additional QC available upon request, including Endotoxin, Western Blot, Mass Spec, ELISA/Activity Assays

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IPTG Fermentation gram milligram E. coli

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Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
Starting at $1,633.00 per unit

Aldevron provides screening, expression, and purification services to isolate your target protein. Standard cell lines include HEK293, CHO-S, HEK293-6E, and CHO-3E7 (EBNA). Additional cell lines (including adherent lines) are available upon request. Aldevron will work with you to select the optimal cell line for your... Show more »

Aldevron provides screening, expression, and purification services to isolate your target protein. Standard cell lines include HEK293, CHO-S, HEK293-6E, and CHO-3E7 (EBNA). Additional cell lines (including adherent lines) are available upon request. Aldevron will work with you to select the optimal cell line for your project.

Mammalian Expression Screening: (Starting at $1672 per target)
• Test recombinant protein expression in one or two cell lines under six (6) conditions.
• Transfect pDNA into mammalian cells, incubate, harvest conditioned media or cell paste (for intracellular proteins).
• Analyze soluble and insoluble fractions by SDS-PAGE.

Mammalian Expression Scale-up: (Starting at $1633 per target)
• 1-75L batch sizes
• Use optimized conditions from expression screen, or standard methods.
• Transfect HEK or CHO cells, prepare culture, induce expression, harvest conditioned media.
• Report results, deliver harvested media OR use media as starting material for purification

Standard Quality Control:
• SDS PAGE
• A280
• RP-HPLC
• Additional QC available upon request, including Endotoxin, Western Blot, Mass Spec, ELISA/Activity Assays

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PEI Transient expression Transfection gram milligram

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Baculovirus/Insect Cell Protein Expression

Starting at $1,250.00 per unit

Aldevron provides screening, expression, and purification services to isolate your target protein. Standard cell lines are Sf9 and Sf21, additional cell lines are available upon request. Aldevron will work with you to select the optimal cell line for your project.

Baculovirus Preparation: (Starting at $1400)
• Generate... Show more »

Aldevron provides screening, expression, and purification services to isolate your target protein. Standard cell lines are Sf9 and Sf21, additional cell lines are available upon request. Aldevron will work with you to select the optimal cell line for your project.

Baculovirus Preparation: (Starting at $1400)
• Generate high-titer baculovirus stock from existing cell line or recombinant transfer vector using standard materials and methods
• Determine viral titer
• Report results and use for expression services

Insect Expression Screening: (Starting at $2070 per target)
• Test baculovirus MOI and TOI in one or two cell lines under six (6) conditions.
• Prepare and infect 30-mL suspension cultures of insect cells (MOI of 0.1, 1 and 10) using standard materials and methods.
• Sample cultures at 48 and 72 hours post infection
• Analyze recombinant protein expression in soluble cell extract by anti-tag Western blot

Insect Expression Scale-up: (Starting at $1250 per target)
• 1-75L batch sizes
• Use optimized conditions from expression screen, or standard methods.
• Infect Sf9 or Sf21 cells, prepare culture, induce expression, harvest cell paste.
• Report results, deliver cell paste OR use paste as starting material for purification

Standard Quality Control:
• SDS PAGE
• A280
• RP-HPLC
• Additional QC available upon request, including Endotoxin, Western Blot, Mass Spec, ELISA/Activity Assays

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WAVE Bioreactor Insects gram milligram

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Protein Purification

Starting at $5,830.00 per run

Aldevron can manage your protein project from gene synthesis through protein characterization. Aldevron offers basic to complex purification and characterization services. IMAC is our standard for first-pass purification, with option to add IEX and/or SEC.

Protein Purification: (Starting at $5,830 per run)
• Purify up to 100... Show more »

Aldevron can manage your protein project from gene synthesis through protein characterization. Aldevron offers basic to complex purification and characterization services. IMAC is our standard for first-pass purification, with option to add IEX and/or SEC.

Protein Purification: (Starting at $5,830 per run)
• Purify up to 100 mg per run by IMAC
• Purify up to 50 mg per run by IMAC-IEX or IMAC-SEC
• Purify up to 25 mg per run by IMAC-IEX-SEC
• Elute into storage buffer using standard materials and methods
• You will receive all protein generated in a production run

Custom Purification:
• Produce protein from >100g to multi-gram scale.
• Elute into storage buffer using standard materials and methods
• You will receive all protein generated in a production run

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Agilent 1100 AKTA FPLC Insects Affinity chromatography Large Scale Chromatography HPLC Preparative HPLC western blot Gradient chromatography HPLC-SEC SDS-PAGE LAL assay Bacterial Endotoxin Bacterial Endotoxin Testing LAL RP-HPLC gram milligram Bacteria E. coli Show 20 more tags Show less

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Therapeutic Monoclonal Antibody (mAb) Development

Starting at $5,830.00 per run

Starting from gene sequence, hybridoma cells, recombinant vectors or stable cell lines, Aldevron can customize a HEK 293 or CHO production process to meet your yield and quality requirements.

Capabilities Include:
• Antibody expression screening, scale-up, and purification.
• Purification development to support scale-up of... Show more »

Starting from gene sequence, hybridoma cells, recombinant vectors or stable cell lines, Aldevron can customize a HEK 293 or CHO production process to meet your yield and quality requirements.

Capabilities Include:
• Antibody expression screening, scale-up, and purification.
• Purification development to support scale-up of multi-gram antibody production
• Antibody sequencing and cloning
• Antigen, scFv, and Fc production available

Expression Screening: (Starting at $1782 per target)
• Test antibody expression in one or two strains under six (6) conditions.
• Transfect mammalian cells, incubate, harvest media.
• Analyze media by SDS-PAGE and A280
• Process 12-90 antibodies in parallel from 50ml to 2L scale

Expression Scale-up (Starting at $2500 per target):
• Use optimized conditions from expression screen, or standard methods.
• Scale-up production from shake flask to WAVE Bioreactor (up to 75 L total volume)
• Transfect HEK or CHO cells, prepare culture, induce expression, harvest conditioned media.
• Report results, deliver harvested media OR use media as starting material for purification

Antibody Purification: (Starting at $5,830 per run)
• Purify up to 200mg of recombinant antibody from conditioned medium by Protein A chromatography
• Formulate in PBS using standard materials and methods
• Determine final purity by analytical SEC (non-reducing conditions) and SDS PAGE (reducing and non-reducing conditions)
• Determine final yield and concentration by A280
• Process limited to 5 L of cell culture per purification (2 L if performed in parallel with other purifications)
• You will receive all antibody generated in a production run

Standard Quality Control:
• SDS PAGE
• A280
• RP-HPLC
• Analytical SEC
• Additional QC available upon request, including Endotoxin, Western Blot, Mass Spec, ELISA/Activity Assays

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AKTA FPLC Oncology Immuno-Oncology Protein A gram milligram Show 6 more tags Show less

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Protein Expression Screening and Optimization

Price on request
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Protein Services

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Protein Production

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Antibody Services

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Biology

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Biochemistry & Molecular Biology

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FPLC

Fast Performance liquid chromatography
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Chemistry and Materials

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Chemistry and Materials Services

Chemistry and Materials Services

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Analytical Chemistry Services

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Analytical Chemistry Services

Analytical Chemistry Services

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Separation/Purification Services

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Separation/Purification Services

Separation/Purification Services

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Chromatography

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Chromatography Services

Chromatography Services

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Liquid Chromatography (LC)

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Liquid Chromatography (LC) Services

Liquid Chromatography (LC) Services

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Protein Formulation

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Formulation Services

Price on request

Formulation Services

Formulation Services

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Antibody Purification

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Project Management

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Business Development, Marketing & Operations

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Business Development, Marketing & Operations Services

Business Development, Marketing & Operations Services

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Business Operations and Logistics Support

Price on request

Business Operations and Logistics Support Services

Business Operations and Logistics Support Services

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Antigen Expression and Purification

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Bioconjugation/Chemical Crosslinking

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Protein Modification

Price on request

Protein Modification Services

Protein Modification Services

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Imaging & Spectroscopy

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Protein Sequencing

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Protein Characterization

Price on request

Protein Characterization Services

Protein Characterization Services

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Justin Byers

Aldevron Client Relations Manager
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Allyson Hauser

Aldevron Director of Client Relations for Protein Services

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