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Affina Biotechnologies, Inc.

ic 100% Positive
ic 23 Orders Completed
Elmsford, New York, US

About Affina Biotechnologies, Inc.

Affina Biotechnologies is dedicated to excellence in helping our customers develop measurements of activities, kinetics and affinities of interactions and concentrations of biomolecules. We offer a unique mix of label-free and label-dependent assay technologies supported by scientific and technical expertise... Show more »

Affina Biotechnologies is dedicated to excellence in helping our customers develop measurements of activities, kinetics and affinities of interactions and concentrations of biomolecules. We offer a unique mix of label-free and label-dependent assay technologies supported by scientific and technical expertise and a wide range of instrumentation including:

Biacore 3000, Pioneer Sensiq, Victor 3V multilabel, HTRF-FRET, Delphia, Lancer, ThermoFisher Multiscan, ACTA FPLC

All clients get a full preliminary consultation on their project, project design and continuous communications. We provide weekly updates and final reports that detail the work performed, provide a summary of the results, analysis of the results, and comments for future plans if appropriate.

Deliverables include: weekly updates, experiments performed at 5 concentrations, K on, K off, and large Kd, full report with description with experiment details, report analysis, and conclusion

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Our Services (52)


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ELISA

Enzyme-linked immunosorbent assay
Starting at $350.00 per plate

Capabilities:
- Kit-based (provided by user), custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization/sonication of cell/tissue lysates
- Transfection/Transduction of non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze... Show more »

Capabilities:
- Kit-based (provided by user), custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization/sonication of cell/tissue lysates
- Transfection/Transduction of non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze fluorescent/chemiluminescent/chromogenic/radioactive substrates

Equipment:
- Multi-channel pipets
- Automatic pipets
- Plate washers

Number of samples processed in a single experiment: In duplicate, 96

Typical turn-around time: 5 days

Troubleshooting protocol: test negative and positive controls, select optimal dilutions for spikes, optimize time and temperature of incubation (all if necessary)

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Surface Plasmon Resonance (SPR)

Price on request

Demonstrate binding for your molecules of interest.
Measure kinetics (kon, koff), affinity (KD), stoichiometry of interaction
Screen antibodies
Analyze detailed kinetics and affinity of antibodies for their antigens
Bin epitopes
Perform standard-free measurement of concentration
Small Molecule Binding Experience
Turnaround time: 1-3 weeks

Demonstrate binding for your molecules of interest.
Measure kinetics (kon, koff), affinity (KD), stoichiometry of interaction
Screen antibodies
Analyze detailed kinetics and affinity of antibodies for their antigens
Bin epitopes
Perform standard-free measurement of concentration
Small Molecule Binding Experience
Turnaround time: 1-3 weeks

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Biacore T-200 Pioneer Sensiq Surface plasmon resonance (SPR) Drug Discovery Biacore

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Protein Expression Screening and Optimization

Price on request

Protein expression and purification. Purification of fully functional proteins supported by our expertise and instrumentation in assay development.

Expression systems:
- E.coli
- insect cells
- in vitro expression

Protein expression and purification. Purification of fully functional proteins supported by our expertise and instrumentation in assay development.

Expression systems:
- E.coli
- insect cells
- in vitro expression

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Protein Purification

Price on request

Protein Purification Capability:
AffinaBio has extensive experience in producing proteins for drug discovery and academic research to be used in exploratory research, drug screens, assays, biomarkers, structural work, and animal studies. We have specific skills in designing proteins strategies for use in biochemical and... Show more »

Protein Purification Capability:
AffinaBio has extensive experience in producing proteins for drug discovery and academic research to be used in exploratory research, drug screens, assays, biomarkers, structural work, and animal studies. We have specific skills in designing proteins strategies for use in biochemical and biophysical characterization and binding studies (SPR, ELISA, FP, HTRF).

AffinaBio designs, develops, and generates structural and functional target recombinant proteins from several groups and across all major therapeutic areas. We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues.

AffinaBio develops and generates recombinant antibodies at the mg and the gram level to be used for in vitro and in vivo studies, at low endotoxin levels.

Support:
Our protein purification support includes:
• Preparative and Analytical chromatography systems, centrifuges, incubators, plate readers
A large selection of chromatography columns, media, and reagents for all aspects of protein validation, detoxification, concentration, chemical and physical modification, and conjugation, etc.

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FRET

Fluorescence resonance energy transfer
Price on request
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Fluorescence Polarization

Price on request
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Monoclonal Antibody (mAb) Production

Price on request
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Time Resolved Fluorescence

Price on request
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Western Blot

Price on request

Turnaround time: 3-4 weeks

Capabilities:
- Homogenization of cell, tissue lysates
- Sonication of cell, tissue lysates
- Fractionate lysates prior to gel loading
- Transfection/Transduction of non-mammalian cells
- In-house cell lines and tissue banks
- Protein concentration determination
- Strip/reprobe membranes
-... Show more »

Turnaround time: 3-4 weeks

Capabilities:
- Homogenization of cell, tissue lysates
- Sonication of cell, tissue lysates
- Fractionate lysates prior to gel loading
- Transfection/Transduction of non-mammalian cells
- In-house cell lines and tissue banks
- Protein concentration determination
- Strip/reprobe membranes
- Commonly used antibodies available
- Optimize protocols for particular antigen/target

Visualization:
- Chromogenic substrates on imager
- Ponceau S staining

Equipment:
- Novex®
- Semi-dry transfers

Number of samples in a single run: 10

We do not have:
- Commonly used antibodies

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Imaging & Spectroscopy

Price on request
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Enzyme Assays

Price on request
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Biology

Price on request
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Protein Production

Price on request

We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues. We are expert at optimizing expression levels modifying tags, time and conditions of expression.

We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues. We are expert at optimizing expression levels modifying tags, time and conditions of expression.

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Project Management

Price on request
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Drug Discovery

Price on request
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Microscopy

Price on request
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Liquid Chromatography (LC)

Price on request
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Pharmacology & Toxicology

Price on request
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Spectroscopy

Price on request
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Protein Quantification

Price on request
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Optical Microscopy

Price on request
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Fluorescence Spectroscopy

Price on request
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Protein-Protein Interaction Analysis

Price on request
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Analytical Chemistry Services

Price on request
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Immunoprecipitation

Price on request
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Affinity Tag Purification

Price on request
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FRET/BRET

Price on request
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Drug Discovery & Development

Price on request
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Biosensor Development

Price on request
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Large Scale Protein Production

Price on request
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Cell-Free Protein Expression

Price on request
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Small Molecule Bioanalysis

Price on request
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Bioanalysis

Price on request
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In vivo Protein Binding Assays

Price on request
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Assay Development Consulting

Price on request
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Kinase Assays

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Assay Development

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Drug Target Validation

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Biochemical Assays

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Process Consulting

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Separation/Purification Services

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Lead Identification and Validation

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Immunoassays

Price on request
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Antibody Services

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Protein Services

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Protein Expression Visualization

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Chemistry and Materials

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Chromatography

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Biomolecular Interaction Analysis

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Medical Devices & Diagnostics

Price on request
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Fluorescence-Based Microscopy

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Biochemistry & Molecular Biology

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Alexander Vinitsky

President, CSO
  • Positive review for Surface Plasmon Resonance (SPR):

    December 2015

    "Alex and his team were very communicative and provided a comprehensive report of the experiments tried. We produced some positive data (in my view) and I would continue the investigation, but plan to pivot the project in another direction (in part as a result of these data, but also further from thinking & reading). Much appreciated! I would reuse their services in the future."

  • Positive review for ELISA:

    December 2013

    "Thanks Alex!"

  • Positive review for ELISA:

    December 2013

    "Quick service, great communication"

  • Positive review for ELISA:

    October 2013

    "Very responsive and proactive for validating antibodies for ELISA"

  • Missing Avatar

    Science Exchange Validation ServiceDecember 2013

    We contacted Alex to perform several antibody validations for our Validation Service. He provided great data within a good timeframe, and was responsive and detailed in his communication. I was most impressed that, even after the projects were completed, he responded to my questions promptly and thoroughly. I had a great experience with Alex and Affina Biotechnologies.

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