1346427153000

Affina Biotechnologies, Inc.

Elmsford, New York, US

Affina Biotechnologies is dedicated to excellence in helping our customers develop measurements of activities, kinetics and affinities of interactions and concentrations of biomolecules. We offer a unique mix of label-free and label-dependent assay technologies supported by scientific and technical expertise and a wide range of instrumentation including:

Biacore 3000, Pioneer Sensiq, Victor 3V multilabel, HTRF-FRET, Delphia, Lancer, ThermoFisher Multiscan, ACTA FPLC

All clients get a full preliminary consultation on their project, project design and continuous communications. We provide weekly updates and final reports that detail the work performed, provide a summary of the results, analysis of the results, and comments for future plans if appropriate.

Deliverables include: weekly updates, experiments performed at 5 concentrations, K on, K off, and large Kd, full report with description with experiment details, report analysis, and conclusion

Affina Biotechnologies, Inc. has not listed any services.

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ELISA
Enzyme-linked immunosorbent assay
Starting at $350.00 per plate

Capabilities:
- Kit-based (provided by user), custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization/sonication of cell/tissue lysates
- Transfection/Transduction of non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze... Show more »

Capabilities:
- Kit-based (provided by user), custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization/sonication of cell/tissue lysates
- Transfection/Transduction of non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze fluorescent/chemiluminescent/chromogenic/radioactive substrates

Equipment:
- Multi-channel pipets
- Automatic pipets
- Plate washers

Number of samples processed in a single experiment: In duplicate, 96

Typical turn-around time: 5 days

Troubleshooting protocol: test negative and positive controls, select optimal dilutions for spikes, optimize time and temperature of incubation (all if necessary)

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Surface Plasmon Resonance (SPR)
Price on request

Demonstrate binding for your molecules of interest.
Measure kinetics (kon, koff), affinity (KD), stoichiometry of interaction
Screen antibodies
Analyze detailed kinetics and affinity of antibodies for their antigens
Bin epitopes
Perform standard-free measurement of concentration
Small Molecule Binding Experience
Turnaround time: 1-3 weeks

Demonstrate binding for your molecules of interest.
Measure kinetics (kon, koff), affinity (KD), stoichiometry of interaction
Screen antibodies
Analyze detailed kinetics and affinity of antibodies for their antigens
Bin epitopes
Perform standard-free measurement of concentration
Small Molecule Binding Experience
Turnaround time: 1-3 weeks

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Biacore T-200
Drug discovery
Biacore
surface plasmon resonance (SPR)
Pioneer Sensiq
SPR
Biacore
Protein Expression Optimization
Price on request

Protein expression and purification. Purification of fully functional proteins supported by our expertise and instrumentation in assay development.

Expression systems:
- E.coli
- insect cells
- in vitro expression

Protein expression and purification. Purification of fully functional proteins supported by our expertise and instrumentation in assay development.

Expression systems:
- E.coli
- insect cells
- in vitro expression

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Protein Purification
Price on request

Protein Purification Capability:
AffinaBio has extensive experience in producing proteins for drug discovery and academic research to be used in exploratory research, drug screens, assays, biomarkers, structural work, and animal studies. We have specific skills in designing proteins strategies for use in biochemical and... Show more »

Protein Purification Capability:
AffinaBio has extensive experience in producing proteins for drug discovery and academic research to be used in exploratory research, drug screens, assays, biomarkers, structural work, and animal studies. We have specific skills in designing proteins strategies for use in biochemical and biophysical characterization and binding studies (SPR, ELISA, FP, HTRF).

AffinaBio designs, develops, and generates structural and functional target recombinant proteins from several groups and across all major therapeutic areas. We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues.

AffinaBio develops and generates recombinant antibodies at the mg and the gram level to be used for in vitro and in vivo studies, at low endotoxin levels.

Support:
Our protein purification support includes:
• Preparative and Analytical chromatography systems, centrifuges, incubators, plate readers
A large selection of chromatography columns, media, and reagents for all aspects of protein validation, detoxification, concentration, chemical and physical modification, and conjugation, etc.

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FRET
Fluorescence resonance energy transfer
Price on request
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Fluorescence polarization
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Monoclonal Antibody (mAb) Production
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Time resolved fluorescence
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Western Blot
Price on request

Turnaround time: 3-4 weeks

Capabilities:
- Homogenization of cell, tissue lysates
- Sonication of cell, tissue lysates
- Fractionate lysates prior to gel loading
- Transfection/Transduction of non-mammalian cells
- In-house cell lines and tissue banks
- Protein concentration determination
- Strip/reprobe membranes
-... Show more »

Turnaround time: 3-4 weeks

Capabilities:
- Homogenization of cell, tissue lysates
- Sonication of cell, tissue lysates
- Fractionate lysates prior to gel loading
- Transfection/Transduction of non-mammalian cells
- In-house cell lines and tissue banks
- Protein concentration determination
- Strip/reprobe membranes
- Commonly used antibodies available
- Optimize protocols for particular antigen/target

Visualization:
- Chromogenic substrates on imager
- Ponceau S staining

Equipment:
- Novex®
- Semi-dry transfers

Number of samples in a single run: 10

We do not have:
- Commonly used antibodies

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Imaging & Spectroscopy
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Enzyme Assays
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Biology
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Experimental Design
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Protein Expression
Price on request

We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues. We are expert at optimizing expression levels modifying tags, time and conditions of expression.

We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues. We are expert at optimizing expression levels modifying tags, time and conditions of expression.

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Project Management, Consulting, & Support Services
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Drug Discovery
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Microscopy
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Liquid Chromatography
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Pharmacology & Toxicology
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Spectroscopy
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Protein Quantification
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Optical Microscopy
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Fluorescence spectroscopy
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Protein-Protein Interaction Analysis
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Analytical Chemistry Services
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Immunoprecipitation
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Affinity Tag Purification
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FRET/BRET
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Drug Discovery & Development
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Biosensor Development
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Large Scale Protein Production
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Cell-Free Protein Expression
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Small Molecule Bioanalysis
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Bioanalysis
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In vivo Protein Binding Assays
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Assay Development Consulting
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Kinase Assays
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Protein Purification and Quantification
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Immunoassay Services
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Assay Development
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Drug Target Validation
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Biochemical Assays
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Process Consulting
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Purification Services
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Lead Identification and Validation
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Immunoassays
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Antibody Services
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Protein Services
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Protein Expression Visualization
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Chemistry and Materials
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Chromatography
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Biomolecular Interaction Analysis
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Medical Devices & Diagnostics
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Fluorescence-Based Microscopy
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Biochemistry & Molecular Biology
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December 8, 2015

Positive review received for Surface Plasmon Resonance (SPR):

"Alex and his team were very communicative and provided a comprehensive report of the experiments tried. We produced some positive data (in my view) and I would continue the investigation, but plan to pivot the project in another direction (in part as a result of these data, but also further from thinking & reading). Much appreciated! I would reuse their services in the future."

December 3, 2013

Positive review received for ELISA:

"Thanks Alex!"

December 3, 2013

Positive review received for ELISA:

"Quick service, great communication"

October 1, 2013

Positive review received for ELISA:

"Very responsive and proactive for validating antibodies for ELISA"

Missing Avatar
2013-12-04 16:02:06 -0500

We contacted Alex to perform several antibody validations for our Validation Service. He provided great data within a good timeframe, and was responsive and detailed in his communication. I was most impressed that, even after the projects were completed, he responded to my questions promptly and thoroughly. I had a great experience with Alex and Affina Biotechnologies.

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