Affina Biotechnologies is dedicated to excellence in helping our customers develop measurements of activities, kinetics and affinities of interactions and concentrations of biomolecules. We offer a unique mix of label-free and label-dependent assay technologies supported by scientific and technical expertise and a wide range of instrumentation including: Biacore 3000, Pioneer Sensiq, Victor 3V multilabel, HTRF-FRET, Delphia, Lancer, ThermoFisher Multiscan, ACTA FPLC All clients get a full preliminary consultation on their project, project design and continuous communications. We provide weekly updates and final reports that detail the work performed, provide a summary of the results, analysis of the results, and comments for future plans if appropriate. Deliverables include: weekly updates, experiments performed at 5 concentrations, K on, K off, and large Kd, full report with description with experiment details, report analysis, and conclusion
Number of samples processed in a single experiment: In duplicate, 96
Typical turn-around time: 5 days
Troubleshooting protocol: test negative and positive controls, select optimal dilutions for spikes, optimize time and temperature of incubation (all if necessary)
Demonstrate binding for your molecules of interest.
Measure kinetics (kon, koff), affinity (KD), stoichiometry of interaction
Analyze detailed kinetics and affinity of antibodies for their antigens
Perform standard-free measurement of concentration
Small Molecule Binding Experience
Turnaround time: 1-3 weeks
Protein expression and purification. Purification of fully functional proteins supported by our expertise and instrumentation in assay development.
Protein Purification Capability:
AffinaBio has extensive experience in producing proteins for drug discovery and academic research to be used in exploratory research, drug screens, assays, biomarkers, structural work, and animal studies. We have specific skills in designing proteins strategies for use in biochemical and biophysical characterization and binding studies (SPR, ELISA, FP, HTRF).
AffinaBio designs, develops, and generates structural and functional target recombinant proteins from several groups and across all major therapeutic areas. We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues.
AffinaBio develops and generates recombinant antibodies at the mg and the gram level to be used for in vitro and in vivo studies, at low endotoxin levels.
Our protein purification support includes:
• Preparative and Analytical chromatography systems, centrifuges, incubators, plate readers
A large selection of chromatography columns, media, and reagents for all aspects of protein validation, detoxification, concentration, chemical and physical modification, and conjugation, etc.
Turnaround time: 3-4 weeks
Number of samples in a single run: 10
We do not have:
We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues. We are expert at optimizing expression levels modifying tags, time and conditions of expression.
"Alex and his team were very communicative and provided a comprehensive report of the experiments tried. We produced some positive data (in my view) and I would continue the investigation, but plan to pivot the project in another direction (in part as a result of these data, but also further from thinking & reading). Much appreciated! I would reuse their services in the future."
"Quick service, great communication"
"Very responsive and proactive for validating antibodies for ELISA"
We contacted Alex to perform several antibody validations for our Validation Service. He provided great data within a good timeframe, and was responsive and detailed in his communication. I was most impressed that, even after the projects were completed, he responded to my questions promptly and thoroughly. I had a great experience with Alex and Affina Biotechnologies.