Affina Biotechnologies, Inc.

Capabilities:
- Kit-based (provided by user), custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization/sonication of cell/tissue lysates
- Transfection/Transduction of non-mammalian cells
- Optimization of protocols for commercial kits
- Analyze serum, plasma, cells, tissues
- Analyze fluorescent/chemiluminescent/chromogenic/radioactive substrates

Equipment:
- Multi-channel pipets
- Automatic pipets
- Plate washers

Number of samples processed in a single experiment: In duplicate, 96

Typical turn-around time: 5 days

Troubleshooting protocol: test negative and positive controls, select optimal dilutions for spikes, optimize time and temperature of incubation (all if necessary)

Demonstrate binding for your molecules of interest.
Measure kinetics (kon, koff), affinity (KD), stoichiometry of interaction
Screen antibodies
Analyze detailed kinetics and affinity of antibodies for their antigens
Bin epitopes
Perform standard-free measurement of concentration
Small Molecule Binding Experience
Turnaround time: 1-3 weeks

Protein expression and purification. Purification of fully functional proteins supported by our expertise and instrumentation in assay development.

Expression systems:
- E.coli
- insect cells
- in vitro expression

Protein Purification Capability:
AffinaBio has extensive experience in producing proteins for drug discovery and academic research to be used in exploratory research, drug screens, assays, biomarkers, structural work, and animal studies. We have specific skills in designing proteins strategies for use in biochemical and biophysical characterization and binding studies (SPR, ELISA, FP, HTRF).

AffinaBio designs, develops, and generates structural and functional target recombinant proteins from several groups and across all major therapeutic areas. We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues.

AffinaBio develops and generates recombinant antibodies at the mg and the gram level to be used for in vitro and in vivo studies, at low endotoxin levels.

Support:
Our protein purification support includes:
• Preparative and Analytical chromatography systems, centrifuges, incubators, plate readers
A large selection of chromatography columns, media, and reagents for all aspects of protein validation, detoxification, concentration, chemical and physical modification, and conjugation, etc.

Turnaround time: 3-4 weeks

Capabilities:
- Homogenization of cell, tissue lysates
- Sonication of cell, tissue lysates
- Fractionate lysates prior to gel loading
- Transfection/Transduction of non-mammalian cells
- In-house cell lines and tissue banks
- Protein concentration determination
- Strip/reprobe membranes
- Commonly used antibodies available
- Optimize protocols for particular antigen/target

Visualization:
- Chromogenic substrates on imager
- Ponceau S staining

Equipment:
- Novex®
- Semi-dry transfers

Number of samples in a single run: 10

We do not have:
- Commonly used antibodies

We generate target recombinant proteins from bacterial, insect, yeast, and mammalian sources, as well as isolating or extracting native proteins from animal tissues. We are expert at optimizing expression levels modifying tags, time and conditions of expression.