The NeuroDiscovery Advanced Tissue Resource Center (ATRC) provides state-of-the-art molecular pathology and neuropathology resources.
Current resources include Laser capture microdissection, DNA/RNA/ncRNA quality/expression analysis and library preparations, Luminex FlexMap 3D multiplex bead cytometry, Digital and real-time PCR, Histopathology services, IHC and photomicroscopy.
The ATRC operates the latest generation Arcturus Veritas and Arcturus PixCell IIe Laser Capture Microdissection System (with fluorescence illumination).
Laser Capture Microscopy — One of the consistent characteristics of neurodegenerative diseases is that they affect specific and stereotyped parts of the nervous system. Laser Capture Microscopy (LCM) enables researchers to precisely isolate material from these regions at the microscopic level. Investigators are able to isolate DNA, mtDNA, mRNA, miRNA and protein from precise populations of neurons. Proteins can then be used directly in experiments, while DNA and RNA samples can be expanded through appropriate amplification techniques.
DNA/miRNA Quality Analysis — A major problem in the use of banked tissue is the variability of recovery and quality of RNA. Experienced users of the various local brain banks report an approximate yield of less than 50% of the material they need. While the underlying biologic issues that explain this poor yield are complicated, it is extremely important to assess nucleic acid quality of a specimen before investing significant time and other resources in subsequent analysis. To provide this 'quality control', the ATRC has an Agilent 2100 Bioanalyzer for unbiased assessment of RNA/DNA quality of tissue samples.miRNA-sized small RNAs can also be visualized with the Agilent bioanalyzer. We have studied the relationship between total RNA stability and miRNA stability using a combination of Agilent bioanalysis, rtPCR and the FlexMiR assay. As such, we are able to reliably assess the quality of miRNA samples prior to expression analyses.
We have recently built upon the ATRC’s expertise by creating a miRNA core facility that allows investigators to examine the central nervous system miRNA profile and determine the relevance of miRNA species to AD and other neurodegenerative diseases. Leading edge technology and expert scientific support dedicated to the neurodegenerative diseases provides ATRC investigators with ready access to this emerging and increasingly relevant area of research.
miRNA services include tissue and LCM-derived miRNA collections and amplifications, and Human/Rodent miRNA expression analyses based on the Luminex FlexMiR assay. We run the FlexMAP 3D instrument, and can perform any multiplex assay designed for said platform.
We also have expertise in creating unique multiplexed Luminex assays from scratch. Specify the antibody pairs and we can develop your assay.
Single and multiplex qPCR services, assay and primer design, full housekeeper and control human brain cDNA collection
Full histopathology core with IHC, Cryotomy, FFPE, anatomical pathology human and animal models
Bench biochemical and biomolecular resource for tissue to library protocols
We are a full molecular pathology core resource specializing in human neural systems but competent in many human and animal models.
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