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Advanced Microscopy/Cytometry Facility

49 Orders Completed
Berkeley, California, US

About Advanced Microscopy/Cytometry Facility

The Advanced Microscopy Facility at the Berkeley Lab is at the core of the Life Sciences Division and provides state of the art facility for fluorescence microscopy, live cell imaging and flow cytometry. Instruments in the facility include two Zeiss LSM710 confocal laser scanning microscopes with live cell... Show more »

The Advanced Microscopy Facility at the Berkeley Lab is at the core of the Life Sciences Division and provides state of the art facility for fluorescence microscopy, live cell imaging and flow cytometry. Instruments in the facility include two Zeiss LSM710 confocal laser scanning microscopes with live cell incubation chamber, and a DeltaVision OMX super-resolution light microscopy system. For flow, the facility provides a Becton-Dickinson Aria sorter and Becton-Dickinson FACS Calibur flow cytometer.

Local Science Exchange users, in the San Francisco Bay Area, may have direct access to the machines upon proper training and review of experiment and samples to be used at the facility.

Full, unassisted, access to FACS Aria and FACS Cailibur will be granted upon training and evaluation from our Cytometry specialist.

Full access to Microscopes will be granted after training and evaluation from our Microscopy specialist.

Slides can be processed at our facility on a per hour basis. Slides need to be prepared following a specific protocol that is sent to users. It is recommended that all users should transfer their acquired data onto an external drive or USB stick after their session has ended.

Our facility deletes all files recorded a month after they have been created. Users are notified before data is deleted so that they can transfer their files if they have not done so before then.

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Our Services (14)


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Flow Cytometry

Starting at $30.00 per hour

Becton Dickinson FACSCalibur (just flow)
Unassisted use $30.00/hour
Operator assisted use $150.00/hour
Becton Dickinson FACSAria (Sorting)
Unassisted use $50.00/hour
Operator assisted use $170.00/hour

Becton Dickinson FACSCalibur (just flow)
Unassisted use $30.00/hour
Operator assisted use $150.00/hour
Becton Dickinson FACSAria (Sorting)
Unassisted use $50.00/hour
Operator assisted use $170.00/hour

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BD FACSAria Cell Sorting System Becton Dickinson FACSCalibur

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Flow Cytometry Data Acquisition

Starting at $150.00 per hour

Becton Dickinson FACSCalibur
2-Laser, 488nm, 645nm
4-color FITC, PE, PerCP, APC

Becton Dickinson FACSCalibur
2-Laser, 488nm, 645nm
4-color FITC, PE, PerCP, APC

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Becton Dickinson FACSCalibur 488nm laser

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TIRF

Total Internal Reflection Microscopy
Starting at $30.00 per hour

The GE OMX super-resolution light microscope is a state of the art instrument capable of resolving fluorescent structures to within 100 nanometers of each other. The system is capable of Structured Illumination (SIM), Total Internal Reflection Fluorescence (TIRF) and PALM-STORM fluorescence imaging. With these techniques... Show more »

The GE OMX super-resolution light microscope is a state of the art instrument capable of resolving fluorescent structures to within 100 nanometers of each other. The system is capable of Structured Illumination (SIM), Total Internal Reflection Fluorescence (TIRF) and PALM-STORM fluorescence imaging. With these techniques resolutions of 100-150 nanometers can be achieved using fluorescent probes. The system is outfitted with four lasers supplying four discreet laser lines for excitation (405nm, 488nm, 561nm, 635nm). It is equipped with a computer controlled stage for precise positioning. The system comes with three very high NA objective lenses for high resolution imaging. The system is computer controlled using API software derived from Delta Vision microscope systems. An integrated workstation provides software algorithms for image deconvolution and reconstruction. Serviced described for 3D SIM in details applies to other modality (i.e. TIRF and PALM-STORM)

Unassisted Use - $30.00/hour
Operator Assisted Use - $150.00/hour

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GE DeltaVision OMX Imaging System

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Confocal Microscopy

Starting at $30.00 per hour

Zeiss LSM710 - 405nm, 458nm, 488nm, 514nm, 561nm, 633nm
Slides and multiwell plate processing

The Zeiss LSM710 is a top of the line laser scanning confocal microscope incorporating a Zeiss Observer Z1 inverted microscope body. The microscope uses a fully automated stage, objective selector, filter cube and autofocus. The... Show more »

Zeiss LSM710 - 405nm, 458nm, 488nm, 514nm, 561nm, 633nm
Slides and multiwell plate processing

The Zeiss LSM710 is a top of the line laser scanning confocal microscope incorporating a Zeiss Observer Z1 inverted microscope body. The microscope uses a fully automated stage, objective selector, filter cube and autofocus. The system is outfitted with four lasers supplying six discreet laser lines for excitation (405nm, 458nm, 488nm, 514nm, 561nm, 633nm). The system uses three Photo-Multiplier-Tubes as detecters incorporating one 32-element spectral detector. The optical collection uses advanced spectral scattering elements to define wavelength selection for optimized multicolor imaging. The system is computer controlled using Zeiss ZEN software controlling all aspects of image excitation and acquisition. The system is capable of single track multicolor and multi-track single/multi-color imaging. The confocal pinhole is computer-controlled helping to optimize pinhole to NA of objective and emission wavelength producing crisp section images. With installed high NA water and oil immersion objective lenses the system is capable of producing high definition 3-dimensional images of cellular organelles and structures. Available modes include Z-stack, FRAP, Time course, Tiles, Regions, Color Deconvolution. LSM710#1 also incorporates an integrated live cell chamber providing a 37C, 5% CO2 humidified environment for extended live cell imaging.
LSM710#2 is exactly the same but does not include a live cell chamber.

Unassisted Use - $30.00/hour
Operator Assisted Use - $150.00/hour

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Zeiss LSM 710 confocal microscope

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3D Structured Illumination Microscopy

Starting at $30.00 per hour

GE DeltaVision OMX Imaging System
3D SIM - TIRF - 405nm, 488nm, 561nm, 635nm

The DeltaVision OMX super-resolution light microscopy system is a system that surpasses previous resolution limits and allows you to image beyond the surface of the coverslip with multiple probes. The system’s structured illumination technology images... Show more »

GE DeltaVision OMX Imaging System
3D SIM - TIRF - 405nm, 488nm, 561nm, 635nm

The DeltaVision OMX super-resolution light microscopy system is a system that surpasses previous resolution limits and allows you to image beyond the surface of the coverslip with multiple probes. The system’s structured illumination technology images more of the biology and resolves features invisible with traditional microscopy. Using the DeltaVision OMX, you can image from five to twenty microns into cells and tissues.

The GE OMX super-resolution light microscope is a state of the art instrument capable of resolving fluorescent structures to within 100 nanometers of each other. The system is capable of Structured Illumination (SIM), Total Internal Reflection Fluorescence (TIRF) and PALM-STORM fluorescence imaging. With these techniques resolutions of 100-150 nanometers can be achieved using fluorescent probes. The system is outfitted with four lasers supplying four discreet laser lines for excitation (405nm, 488nm, 561nm, 635nm). It is equipped with a computer controlled stage for precise positioning. The system comes with three very high NA objective lenses for high resolution imaging. The system is computer controlled using API software derived from Delta Vision microscope systems. An integrated workstation provides software algorithms for image deconvolution and reconstruction.

Unassisted Use - $30.00/hour
Operator Assisted Use - $150.00/hour

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GE DeltaVision OMX Imaging System

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Photoactivated Localization Microscopy

Starting at $30.00 per hour

The GE OMX super-resolution light microscope is a state of the art instrument capable of resolving fluorescent structures to within 100 nanometers of each other. The system is capable of Structured Illumination (SIM), Total Internal Reflection Fluorescence (TIRF) and PALM-STORM fluorescence imaging. With these techniques... Show more »

The GE OMX super-resolution light microscope is a state of the art instrument capable of resolving fluorescent structures to within 100 nanometers of each other. The system is capable of Structured Illumination (SIM), Total Internal Reflection Fluorescence (TIRF) and PALM-STORM fluorescence imaging. With these techniques resolutions of 100-150 nanometers can be achieved using fluorescent probes. The system is outfitted with four lasers supplying four discreet laser lines for excitation (405nm, 488nm, 561nm, 635nm). It is equipped with a computer controlled stage for precise positioning. The system comes with three very high NA objective lenses for high resolution imaging. The system is computer controlled using API software derived from Delta Vision microscope systems. An integrated workstation provides software algorithms for image deconvolution and reconstruction. Serviced described for 3D SIM in details applies to other modality (i.e. TIRF and PALM-STORM)

Unassisted Use - $30.00/hour
Operator Assisted Use - $150.00/hour

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GE DeltaVision OMX Imaging System

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Microscopy

Price on request
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Biology

Price on request
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Fluorescence-Based Microscopy

Price on request
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Super Resolution Microscopy

Price on request
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Cytometry

Price on request
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Optical Microscopy

Price on request
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Cells and Tissues

Price on request
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Imaging & Spectroscopy

Price on request
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David Acevedo

Current Director of the Advanced Microscopy Facility

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  • Positive review for Confocal Microscopy:

    March 2017

    "Great facility for microscopy experts to perform commercial work supporting their research objectives."

Advanced Microscopy/Cytometry Facility has not received any endorsements.