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Advanced Imaging Core (AIC)

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Chicago, Illinois, US

About Advanced Imaging Core (AIC)

The Center’s Advanced Imaging Core (AIC) features state-of-the-art core technologies available on a fee for service basis.

Our Services (6)


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Light Sheet Microscopy

Price on request

The Digital Scanned Laser Light Microscope (DSLM) is an advanced live-imaging fluorescence technology recently developed by Dr. Stelzer’s group at EMBL, Germany.

This new microscope combines very important features including high imaging speed, low photo-load on the specimen, excellent signal-to-noise ratio and multi-view... Show more »

The Digital Scanned Laser Light Microscope (DSLM) is an advanced live-imaging fluorescence technology recently developed by Dr. Stelzer’s group at EMBL, Germany.

This new microscope combines very important features including high imaging speed, low photo-load on the specimen, excellent signal-to-noise ratio and multi-view imaging capability. The latter feature refers to the capability of imaging a specimen from multiple directions, which is crucial in examining large specimens with high light scattering or light absorption. The system also incorporates live speed structured (spatially modulated) illumination further increasing the spatial resolution of resulting images.

The main distinguishing features of the DSLM are the following:
- Illumination and detection functions are separated between two dedicated objectives.

  • To decrease photo-toxicity and increase scanning speed, the illuminated field is limited to a narrow, several microns thick, “light sheet”.

In traditional microscopy, the whole volume of the sample is illuminated by moving the laser beam in both x and y directions, which is performed multiple times for z-stack collection. By contrast, in the DSLM, only thin light sheets that are being imaged are illuminated one at a time by moving the laser beam along just one direction. This way, the photo-toxicity is decreased dramatically and the speed of the imaging, not limited by the speed of the scanning laser, is increased a hundred fold and more. Live imaging becomes possible with higher brightness and contrast and over considerably longer periods of time.

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Fluorescence-Based Microscopy

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Live Cell Imaging

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Imaging & Spectroscopy

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Optical Microscopy

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Microscopy

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