1389324893793 59c52691750c179e34cc162d6ccb96e0

ADS Biosystems Inc.

ic 7.7/10 Overall Rating 80% Positive
ic 14 Orders Completed
San Diego, California, US

About ADS Biosystems Inc.

ADS Biosystems Inc. has a very experienced and highly skilled team and provides a wide range of services for academia and industry customers. We have a number of repeat customers, such as Celgene, Genentech, Sanofi Europe, UCSD, Intercept, and Third Rock Ventures, just to name a few. Our featured services... Show more »

ADS Biosystems Inc. has a very experienced and highly skilled team and provides a wide range of services for academia and industry customers. We have a number of repeat customers, such as Celgene, Genentech, Sanofi Europe, UCSD, Intercept, and Third Rock Ventures, just to name a few. Our featured services include, but not limited to, antigen-based affinity antibody purification, in vitro kinase assays, Alpha Screen, and custom ELISA kit formation and testing . We specialize in cell-based assays, such as human M1 macrophage Caspase 1 activation, and many other luciferase-based assays in cells, such as FXR based luciferase assays in cells. Our expertise is on human CD34+ hematopoietic stem cells and differentiation into human T cells. We specialize in cell-based assays, such as luciferase assays, in a variety of primary cells, cell lines and tissue cultures. We also provide molecular biology services such as real time RT-PCR, quantitative Western Blot and ELISA, and human cell mutation tests; cell biology services such as stable cell lines with designated reporters, cell differentiation studies with brown and white adipocytes, human adult stem cells, and cochlear organ culture for hearing studies; in vivo mouse model studies for obesity and diabetes. We are capable of testing drug candidates for hearing loss studies in our chemically deafened cochlear cultures, and for obesity and diabetes in vitro with adipocytes and in vivo with mouse models.

Sample shipment instructions:
Tissues and cells on dry ice

Services:
- Custom genomic deletion and mutation tests of human cells and cell lines
- Gene expression profiling and real-time RT PCR (Taqman assays) of human tissues and cell lines
-Protein expression with quantitative Western blot analyses and ELISA
- Primary cell culture and cochlear organ culture
- Custom stable cell lines from human and murine adult stem cells
- Custom testing for brown and white fat differentiation in primary human cultures
- Custom lentiviral-mediated human stable cell lines with different reporters such as luciferases
- Custom shRNA gene knockdown in human and mouse stable cell lines
- In vivo custom studies of the effects of high fat diet on commercial and/or custom genetic mouse
models for obesity and diabetes and in vivo rodent glucose and insulin tolerance tests

Assay development**:
- Custom biochemical and cell-based assay development; routine human and mouse cell-based assays
with custom reporters and endogenous gene expression such as Northern blot analyses, real time RT
PCR and protein level detection with quantitative Western blot assays and ELISA; quantitative assays
for variations in endogenous signal transduction pathways; specialty assay development for human
brown and white adipocyte differentiation in obesity and diabetes, and cochlear organ culture in hearing loss and restoration.

**We are happy to buy commercially available animal models, obesity/diabetes models, cochlea hearing mouse model, tumor models, breast cancer models. We also have access to the following cell lines: cancer cell lines, lung, breast, other routine cell lines like leukemia and lymphoma, fibroblast, mostly human. Our main focus is primary cells, and we can create genetically modified models, mouse fibroblast, etc.

Preclinical drug discovery and development:
- Custom testing of drug candidates for efficacy in human and mouse cell lines, human adult stem cells and cochlear cultures with designated reporters, endogenous gene expression and effects on endogenous signal transduction pathways; in vivo testing of drug candidates in rodents.

Instrumentation:
- Li-cor Odyssey: detects fluorescence, quantifies the signal, very sensitive up to several magnitudes, used to quantify individual bands to compare to loading control

References:
- Wang Z, Shah OJ, Hunter T (2012). The transcriptional coactivators p/CIP and SRC-1 control insulin resistance through IRS1 in obesity models. PLoS One.2012;7(7):e36961. Epub 2012 Jul 31.
- Wang Z, Qi C, Krones A, Woodring P, Zhu X, Reddy JK, Evans RM, Rosenfeld MG, and Hunter T. (2006). In vivo roles of the p160 transcriptional coactivators p/CIP and SRC-1 in energy balance. Cell Metabolism 3:111-122.
- Shah OJ, Wang Z, Hunter T. (2004). Inappropriate activation of the TSC/Rheb/mTOR/S6K cassette induces IRS1/2 depletion, insulin resistance, and cell survival deficiencies. Current Biology 14:1650
- Lu Z, Ghosh S, Wang Z, Hunter T. (2003). Downregulation of caveolin-1 function by EGF leads to the loss of E-cadherin, increased transcriptional activity of beta-catenin, and enhanced tumor cell invasion. Cancer Cell. 4:499
- Wang Z, Rose DW, Hermanson O, Liu F, Herman T, Wu W, Szeto D, Gleiberman A, Krones A, Pratt K, Rosenfeld R, Glass CK, Rosenfeld MG. (2000). Regulation of somatic growth by the p160 coactivator p/CIP. Proc Natl Acad Sci U S A. 97:13549

Patents:
- Thies S, Farouz F, Wang Z, Jacques B, and Lu J. Small molecule compounds to treat hearing loss. International publication number: WO2013/126805A2
- Wong L, Xu S, Ding J. Biomarker Assays for detecting or measuring inhibition of TOR kinase activity. Patent application number: 20120028972
- Wisniewski M, Kwong W, Mohsenian F, Ding J. Nucleic acid preparation composition and methods. International publication number: WO 2010/115016 A2

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Our Services (20)


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Custom Cell-Based Assay Development

Starting at $100.00 per hour

Custom cell-based assay development:
- Stable cell line with designated reporters
- Human stem cell assays
- Brown and white fat cell-based assays
- we offer luciferase assays as part of cell-based assay services, not stand alone service


Turnaround time: 1-3 weeks


Custom cell-based assay development:
- Stable cell line with designated reporters
- Human stem cell assays
- Brown and white fat cell-based assays
- we offer luciferase assays as part of cell-based assay services, not stand alone service


Turnaround time: 1-3 weeks


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In vitro Hearing Loss Model

Starting at $100.00 per hour
  • Cochlear organ culture
  • Chemically-induced hearing loss model in vitro
  • Drug candidates testing in cochlear organ culture
    _______

Turnaround time: 4 weeks


*Price is $100/hour plus materials and reagents

  • Cochlear organ culture
  • Chemically-induced hearing loss model in vitro
  • Drug candidates testing in cochlear organ culture
    _______

Turnaround time: 4 weeks


*Price is $100/hour plus materials and reagents

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ELISA

Enzyme-linked immunosorbent assay
Price on request
Request a quote for more information about this service.

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Elisa Kit Development

Price on request
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Custom Affinity Purification of Antibodies

Price on request

Antigen-based affinity antibody purification from anti serum

Custom built affinity column with your antigen, and purification of high affinity antibody for Western blot, ELISA, and immunostaining studies.

Antigen-based affinity antibody purification from anti serum

Custom built affinity column with your antigen, and purification of high affinity antibody for Western blot, ELISA, and immunostaining studies.

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Western Blot

Starting at $300.00 per gel

Determine effects of genetic variations, drugs, different culturing treatments on signal transduction pathways and protein levels.


Turnaround time: 1-2 weeks


Sample requirements:

  • If the experiment is started from cell lines or from our own lysate, we can guarantee a high quality of lysate. If the... Show more »

Determine effects of genetic variations, drugs, different culturing treatments on signal transduction pathways and protein levels.


Turnaround time: 1-2 weeks


Sample requirements:

  • If the experiment is started from cell lines or from our own lysate, we can guarantee a high quality of lysate. If the researcher is providing their own lysate, they must use protease inhibitors and phosphatase inhibitors when making the lysate.

  • Samples should be sent as tissue or pellet, and should be frozen quickly (1-2 seconds) in liquid nitrogen, then shipped on dry ice.


Deliverables:
- report with two parts: three kinds of color images (loading control, protein expression band, composite of the two), plus quantification of each individual band in each lane, as well as the ratio of the expression band to the loading control (not just the raw data)
- samples returned upon request (they are kept in -80oC or -20oC as appropriate, and can be sent back using a dry ice shipment)

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qRT-PCR

Quantitative reverse transcription polymerase chain reaction
Starting at $100.00 per sample

Service includes RNA isolation, cDNA production, and Taqman assays. Taqman primers and probe not included, but can be obtained from Life Technology.

Service includes RNA isolation, cDNA production, and Taqman assays. Taqman primers and probe not included, but can be obtained from Life Technology.

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Biology

Price on request
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Specialized Cell-Based Assays

Price on request
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Protein Quantification

Price on request
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Cell-Based Assays

Price on request
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In vitro Disease Models

Price on request
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Protein Expression Visualization

Price on request
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PCR

Price on request
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Cell Lines

Price on request
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Animal Models and Studies

Price on request
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Protein Purification

Price on request

Protein Purification and Quantification Services

Protein Purification and Quantification Services

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Assay Development

Price on request
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RT-PCR

Reverse transcription polymerase chain reaction
Price on request
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TaqMan Assays

TaqMan Gene Expression Assays
Price on request
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Western Protocol for Odyssey12.docx

  • Positive review for Western Blot:

    May 2018

    "ADS Biosytems was very responsive and had quick turn-around time for producing the results. The quality of the Western blots was excellent."

  • Positive review for ELISA:

    October 2016

    "Excellent work, professional and knowledgeable, would highly recommend!"

ADS Biosystems Inc. has not received any endorsements.