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Active Motif Inc.

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Carlsbad, California, US

About Active Motif Inc.

Active Motif is the industry leader in developing and delivering innovative tools to enable epigenetics and gene regulation research. We are committed to providing the highest quality products and superior service & support to serve the life science, clinical and pharmaceutical/drug discovery communities.... Show more »

Active Motif is the industry leader in developing and delivering innovative tools to enable epigenetics and gene regulation research. We are committed to providing the highest quality products and superior service & support to serve the life science, clinical and pharmaceutical/drug discovery communities. Whether you are an expert in the field of epigenetics or a researcher interested in integrating epigenetics research into your studies, Active Motif offers a comprehensive portfolio of epigenetics-related products and services and the support of our team of epigenetic experts to provide complete and innovative solutions to tackle your scientific inquiries.

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Our Services (51)


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Biochemical Reagents

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Next Generation Sequencing (NGS)

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Multiplex Gene Expression Analysis

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ChIP-qPCR

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Active Motif performs custom ChIP-qPCR assays to provide quantitative data on transcription factor binding and histone modification occupancy. Our Epigenetic Services team performs thousands of ChIP reactions every year. In order to make all of our experiments comparable and establish criteria for success, we have devised a unique... Show more »

Active Motif performs custom ChIP-qPCR assays to provide quantitative data on transcription factor binding and histone modification occupancy. Our Epigenetic Services team performs thousands of ChIP reactions every year. In order to make all of our experiments comparable and establish criteria for success, we have devised a unique normalization strategy. This normalization strategy, along with special attention dedicated to evaluating signal intensity and background levels, results in the highest quality ChIP data available.

The ChIP-qPCR Service includes:

Customers submit frozen tissues or cell pellets, then we:

  1. Prepare chromatin samples and sonicate.
  • Perform ChIP with a ChIP-qualified antibody.
  • Design and synthesize primers.
  • Perform qPCR reactions in triplicate for the customer-selected binding sites and positive & negative control regions.
  • Perform control qPCR reactions in triplicate using input DNA (unprecipitated genomic DNA).
  • Analyze the data and deliver it to the customer.
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ChIP-Seq

Chromatin Immunoprecipitation Sequencing
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Customers submit fixed cell pellets or frozen tissues, then we:

  1. Prepare chromatin samples and sonicate.
  • Perform ChIP with a ChIP-qualified antibody.
  • Construct ChIP-Seq libraries.
  • Perform Next-Gen sequencing.
  • Analyze & deliver the data.

Customers submit fixed cell pellets or frozen tissues, then we:

  1. Prepare chromatin samples and sonicate.
  • Perform ChIP with a ChIP-qualified antibody.
  • Construct ChIP-Seq libraries.
  • Perform Next-Gen sequencing.
  • Analyze & deliver the data.
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ATAC-Seq

Assay for Transposase-Accessible Chromatin with High Throughput Sequencing
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ATAC-Seq is based on transposase-mediated insertion of sequencing primers into open chromatin regions. This assay provides genome-wide profiles of open and accessible regions of chromatin that are indicative of active regulatory regions.

Why study Open Chromatin?

  • Gain mechanistic insight into gene regulation, cellular... Show more »

ATAC-Seq is based on transposase-mediated insertion of sequencing primers into open chromatin regions. This assay provides genome-wide profiles of open and accessible regions of chromatin that are indicative of active regulatory regions.

Why study Open Chromatin?

  • Gain mechanistic insight into gene regulation, cellular response to treatment or disease
  • Identify which transcription factors are driving cell fate, disease, or response
  • Primary tissues or cells such as pancreatic Beta cells
  • Limited patient samples
  • Stratify patients or sample groups based on open chromatin signatures

ATAC-Seq can be a good alternative to ChIP-Seq if it is unknown whether epigenetics plays a role in the response of your cell system, if it is unclear which histone modification is the most important to study using ChIP-Seq or when cell numbers are limited.

The ATAC-Seq assay includes;

  1. Cell preparation
  • Transposase reaction
  • Library amplification
  • Sequencing on an Illumina platform
  • Bioinformatic analysis

Sample Types

Active Motif’s services team is the only group routinely generating ATAC-Seq data from tissues. Active Motif will accept the following sample types for this service:

  1. Human and animal tissues (including xenografts and human biopsies)
  • Primary cells (including T and B cells)
  • FACS sorted cells
  • Most rare cell populations
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RNA Sequencing

Price on request

RNA-Seq is a powerful tool for measuring gene expression changes with many applications including:

  • Assessing response to treatment or disease.
  • Identification / characterization of complete transcriptomes.
  • Investigation of protein coding (mRNAs) and non-coding RNAs (ncRNAs).
  • Identification of novel transcript... Show more »

RNA-Seq is a powerful tool for measuring gene expression changes with many applications including:

  • Assessing response to treatment or disease.
  • Identification / characterization of complete transcriptomes.
  • Investigation of protein coding (mRNAs) and non-coding RNAs (ncRNAs).
  • Identification of novel transcript isoforms, variants, and alternative splicing events.

Active Motif provides a comprehensive RNA-Seq service that includes everything from isolation of RNA to data analysis. Combine our worldwide leading ChIP-Seq services with RNA-Seq to gain an even greater understanding of your biological system.

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Reduced Representation Bisulfite Sequencing (RRBS)

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Reduced Representation Bisulfite Sequencing (RRBS) is a method that facilitates the identification of differentially methylated regions by providing DNA methylation data at single base pair resolution on greater than 4 million CpGs. More cost effective than Whole Genome Bisulfite Sequencing and with greater coverage than bead... Show more »

Reduced Representation Bisulfite Sequencing (RRBS) is a method that facilitates the identification of differentially methylated regions by providing DNA methylation data at single base pair resolution on greater than 4 million CpGs. More cost effective than Whole Genome Bisulfite Sequencing and with greater coverage than bead array based DNA methylation platforms, RRBS is an ideal platform to profile DNA methylation across samples.

Highlights:

  • Data on 3 to 5 million CpGs per sample.
  • Biologically relevant target regions including promoters and CpG islands.
  • Sequencing depth of > 30,000,000 reads.
  • Low input DNA requirement of 1 µg.
  • Reduced library bias resulting from random bar code incorporation.

Deliverables:

  • Raw data (FASTQ) output files.
  • Aligned data (BAM) files.
  • Visualization files
    – BAM files can be used for visualization inIGV (Integrated Genome Viewer)
  • Methylation table includes percent methylation value for each CpG.
  • Differential methylation analysis.
  • Figures & graphs
    – Includes coverage depth analysis, heatmaps & pie charts
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Custom Monoclonal Antibody (mAb) Production

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New technologies and high-throughput approaches have created exciting opportunities at the intersection of genomics and immunology. Using next-gen sequencing, Active Motif has developed a novel method to profile the complete antibody repertoire of an organism. We have also created new gene assembly methods to convert immune... Show more »

New technologies and high-throughput approaches have created exciting opportunities at the intersection of genomics and immunology. Using next-gen sequencing, Active Motif has developed a novel method to profile the complete antibody repertoire of an organism. We have also created new gene assembly methods to convert immune repertoire sequence information into large collections of full IgG antibodies that can be screened for function.

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Cell Line Generation

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Luminex Multiplex Assays

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Mass Spectrometry

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Rapid Immunoprecipitation Mass Spectrometry of Endogenous Proteins (RIME)

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RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) is a recently developed technique ideally suited for the identification of transcriptional co-factors and chromatin associated proteins.

The RIME Service includes:

  1. Nuclear isolation and sonication.
  • Immunoprecipitation.
  • Purification of proteins and tryptic digestion.
  • Mass spectrometry.
  • Data analysis.

RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) is a recently developed technique ideally suited for the identification of transcriptional co-factors and chromatin associated proteins.

The RIME Service includes:

  1. Nuclear isolation and sonication.
  • Immunoprecipitation.
  • Purification of proteins and tryptic digestion.
  • Mass spectrometry.
  • Data analysis.
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Antibody Services

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Mammalian Cell Culture

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DNA Extraction and Purification

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Biochemical Assays

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Cells and Tissues

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DNA Methylation Analysis

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Histone Modification Analysis

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Global Methylation Analysis

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Protein-Protein Interaction Analysis

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ChIP Data Analysis

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Pharmacology & Toxicology

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Protein-DNA Interaction Analysis

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DNA Sequencing

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Spectroscopy

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Targeted Gene Sequencing

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DNA Services

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Biomolecular Interaction Analysis

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Protein Services

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Chromatin Immunoprecipitation (ChIP) Assays

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Biochemical Assay Kits

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DNA Amplification

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ChIP-ChIP

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Chromosome Conformation Capture

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MicroRNA (miRNA) Expression Profiling

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Whole Genome Sequencing

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RNA Services

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RNA Profiling

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Whole Genome Amplification

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DNA Purification

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Targeted Sequencing

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Methyl Binding Domain (MBD)-Enriched Genome Sequencing

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Lysine Specific Demethylase-1 (LSD-1) Assays

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Biology

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Imaging & Spectroscopy

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Biochemistry & Molecular Biology

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Nucleic Acid Services

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Bioanalysis

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Epigenetic Studies

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