ACGT, Inc., is a DNA sequencing and molecular biology service company founded in 1993. Our laboratories are located in Wheeling, Illinois and Germantown, Maryland. We provide a suite of genetic and genomic services, including DNA sequencing by Sanger and Next Generation Sequencing. All services are offered at a research, GLP or clinical grade levels. Our facilities are accredited by the College of American Pathologists (CAP) according to the standards set forth in the Clinical Laboratory Improvement Amendments (CLIA). [![YOUTUBE VIDEO](http://img.youtube.com/vi/a-T_QhbXooM/0.jpg)](https://www.youtube.com/watch?v=a-T_QhbXooM)
Discounted Pricing From November 1, 2018 through December 21, 2018
|Sequencing on the Illumina HiSeq 4000||Millions of Reads/Flow Cell||** Read Length**|
|HiSeq 400 sequencing -Single Read||2500||50|
|HiSeq 4000 sequencing- Paired End Read||2500||2x50|
Quick Single Pass DNA Sequencing
Direct Colony Sequencing
Instead of purified plasmid DNA, just send us bacterial colonies re-suspended in a small volume of buffer and a sequencing primer (separately or in the same tube). Our skilled professionals will extract and amplify the plasmid DNA directly from the colony, and generate sequencing data with the same exceptional level of quality as our standard sequencing service.
Full Sequence Analysis
ACGT, Inc. offers a Full Sequence Analysis service to confirm the sequences of a clone or detection of mutations or deletions. Our service is comprehensive and results in double-strand sequence data of publication quality. Service consists of the primer walking sequencing method in double-strand or single-strand format. This service includes primer design and synthesis.
High Throughput Sequencing
ACGT, Inc. offers High throughput DNA sequencing in a fully automated lab. We are capable of handling thousands of clones per day using a barcode system to accurately inventory and process DNA samples upon receipt until delivery of final data. Capability to handle up to 3,500 samples per day with numerous ABI 3730XL analyzers. We accept all sample types for preparation of plasmid DNA template: ligation mixtures, transformation supernatants, bacterial cultures, colonies on agar plates, or frozen glycerol stocks.
Intact, high-quality genomic DNA (gDNA) is routinely extracted from blood, saliva, buccal swabs, soft tissues, or plants. We also perform total nuclei acid extractions where gDNA, plasmid DNA and RNA are concomitantly isolated from the same sample such as bacteria. For DNA extraction from plants, the Automated Sample Grinding System (AutoGen) is used to obtain consistent results. Extraction of gDNA from rodent tails or ears is part of our transgenic genotyping service.
DNA Extraction Service Description:
We offer a complete Genotyping by DNA Sequencing for screening alleles based on nucleotide sequence. Alleles in any organism are detected by PCR amplification of the target gene and DNA sequencing of the resulting PCR product. Dilution PCR analysis or bulk PCR product subcloning and screening followed by DNA sequencing is an additional service provided for reliable analysis of single point mutations in low abundance. DNA sequencing is one of the most definitive methods available for allele identification and genotyping.
Genotyping by DNA Sequencing Service Description:
ChIP-sequencing (ChIP-Seq) combines chromatin immunoprecipitation (ChIP) and massively parallel sequencing to identify those DNA sequences bound by transcription factors in vivo.
A very common form of ChIP-Seq experiment is the Histone Modification Analysis. Histone compaction of DNA is a well-known epigenetic regulator of gene expression. Post-translational modification of histones, such as histone deacetylation, is another common form of epigenetic control, and is known to be associated with gene silencing. Therefore, evaluation of histone coverage and histone modification status are valuable tools in understanding genome-wide regulation of gene expression.
We offer primarily Targeted Metagenomics services. Targeted metagenomics typically aim at smaller-scale goals, such as the 16S rRNA-based surveys for microbe identification, or acquiring sequence reads with specific protein functions, such as antibiotic resistance genes.
How it works: Total DNA is extracted from the sample, followed by any number of selected PCR reactions. The short PCR products (300 bp maximum size) are purified and are converted to Illumina libraries by the ligation of adapters. Multiplex barcoding is typically used to maximize sample processing efficiency. The libraries are analyzed using long paired-end reads to fully sequence each PCR product in the cluster. The reads are sorted, counted, and non-identical sequences are BLASTed against selected databases for sequence identification.
Epigenetic control is often mediated by methylation of cytosine to 5-methylcytosine (5-mC) in CpG islands. Methylation and/or hydroxymethylation of CpGs near promoters is associated with gene silencing, and has important consequences for gene expression by contributing to the re-modeling of chromatin via recruitment of methyl-CpG-binding domain (MBD) protein complexes and subsequent chromatin modifiers. Disease phenotypes have been shown to arise when these activities are perturbed, resulting in undesired gene expression.
DNA methylation status can be evaluated in two basic ways:
High quality plasmid DNA from the Mini to Giga scale is available for many applications including DNA sequencing, transfections, and DNA-mediated antibody production. Resin based methods are used to prepare highly pure plasmid DNA preparations with an option of making these endotoxin-free. Comparable services are available for BAC, lambda, and cosmid DNA.
Extraction of highly pure and intact RNA (total, mRNA, sRNA and miRNA) from a variety of samples including soft tissues (fresh and preserved), blood, saliva, cell cultures, plants, and forensics samples is available whether it from a few samples to large-scale project. Both liquid and resin based purification systems are used to extract the RNA species of interest. Concomitant extraction of genomic DNA and plasmid DNA is possible. The purified RNA is suitable for many downstream applications including cDNA synthesis including library construction, Northern blots and qPCR.
Complete sequencing of transcriptomes has become an important tool in the analysis of gene expression, alternative splice sites, allele specific expression and the discovery and analysis of rare or novel transcripts. Unlike the microarray approach which analyzes the known transcripts contained on the array, with the NGS approach the entire RNA population contained in the sample is sequenced. This provides an unprecedented detail of the transcriptome organization and structure.
We create a random mutagenesis library of any desired complexity to any gene of your interest.
We offer Single Nucleotide Polymorphism (SNP) genotyping for large and small scale projects including SNP discovery, validation, and screening. Accurate and reproducible results are more accessible for projects of any size with the use of an established platform and optimized. SNPs from any organism can be genotyped.
SNP Genotyping Service Description:
We offer a complete Fragment Analysis of Short Tandem Repeats (STR) or microsatellite (MS) genotyping service. Use of multiple ABI 3730XL or 3730 Genetic Analyzers allows us to screen hundreds of samples per day. STR Genotyping is available for most organisms.
STR Genotyping Service Description:
We provide shotgun sequencing services for a variety of samples including BAC, PAC, or cosmid DNA for genomic scale projects.
You will receive assembled and based-called DNA sequences, publication-ready DNA sequences, electropherograms of each run, and all of the subclones, plasmid DNA, and internal primers generated for the project.
Based on PCR primer design, any type of mutation, such as deletions, insertions, or substitutions, can be introduced into your genes in a single or multiple sites. Any desired changes in amino acids, or deletions and insertions of restriction sites can be made into the genes of your interest.
We offer whole genome sequencing on NextSeq 500 platform, with PE150 reads. Sequencing costs for a bacterial genome are in the $200 range for 100X coverage; for human and similar size genomes, it is $5,200 for 35X coverage.
cDNA Cloning Service Description:
We offer a rapid, reliable semi-quantitative and quantitative PCR (qPCR) analysis of gene expression and gene copy number determination. Detection of targets with Taqman™ probes with the 7900HT Sequence Detection System (Applied Biosystems Inc.) or SYBR green is available for large or small scale projects alike. The instrument platform has high-throughput capabilities and can analyze over 1,500 samples on a daily basis.
The full service option includes RNA or DNA extraction, probe design and optimization and complete data analysis including normalization of samples with internal controls. Relative and absolute analysis of gene expression by qPCR is available for all known genes in any organism.
"They were fast, professional and helpful in all ways."
"Great service. Love the prompt responses and the explanations. My only wish is that the assembled FASTA files were complementary. Nevertheless, I would recommend this company g for the quick turn around, prompt and clear communication and a slightly competitive cost for WGS...not so much the cost for analysis."
"ACGT uses well-established kits and standard protocols. They have been very helpful in trying to trouble-shoot problems during the protocols. Their experience in NGS is reliable."
"When I first accepted a quote for whole genome sequencing and analysis including SSR mining, I did so based on their estimated turn-around time of 2-3 weeks. Four weeks after ACGT, Inc. had checked the quality of the DNA sample that I had sent, I checked in to see where things stood with my order, expecting that my data would be ready. A representative from ACGT, Inc. responded that their sequencing machine was currently down and they had not been able to proceed. I finally received my sequencing data and analysis on 7/22/15, nearly 4 weeks later than their three week estimate. Upon inspection of the analysis their team provided, I found that the paired-end reads had not been assembled prior to mining for SSRs, which led to major redundancy issues. Using the same search parameters as ACGT, Inc., I was able to find approximately 37,000 SSRs as opposed to the 2.8million found by ACGT, Inc. To be fair, when I brought my concerns to their team, they did work to address this issue and reduced the amount charged for analysis. That said, I am unable to recommend their services. As for Science Exchange, I worked with Risha Shah, who was highly supportive throughout this process."
"Members of this lab were extremely responsive and knowledgeable. They dedicated time to answer detailed questions and even suggest good solutions based on their expertise. The price was also very competitive."
"Thank you ever so much!"
ACGT, Inc. (CLIA Compliant) has not received any endorsements.