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Accelagen, Inc.

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San Diego, California, US

About Accelagen, Inc.

Accelagen is a fast growing Gene-to-Protein company providing superior quality services and products. The Accelagen team has demonstrated independent research capability in many projects and provided innovative solutions. Our scientists have extensive experiences in production of antibodies, GPCRs, kinases, nuclear receptors, and many other classes of drug targets and therapeutic proteins. Accelagen has established an unsurpassed track record of timely completion of projects over the years.

Accelagen contract services provide a comprehensive gene-to-protein solution. Our recombinant protein expression and purification services have yielded premium quality recombinant proteins for drug discovery. We have produced recombinant proteins for determination of co-crystal structures in Structure-Based Drug Discovery (SBDD), for assays in high-throughput screening (HTS) and lead development, and for pre-clinical animal studies.

Accelagen’s gene-to-protein platform integrates the latest technologies of Baculovirus Expression Vector System (BEVS), E. coli expression, mammalian expression, Wave Bioreactors, Cell Factory, and AKTA chromatography systems for protein purificaiton. We have achieved superior protein quality and accelerated the processes from gene to protein. This platform is validated in the quality of our products and by the successes of our customers.

Our mission is to provide cost-effective gene-to-protein services, through technical excellence and quality communication.

Our Services (19)


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Gene-to-Protein Services

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BacMam Protein Expression

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Protein Purification

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Accelagen’s protein experts have accumulated extensive working knowledge of purifying recombinant proteins for drug discovery in companies like Agouron (now Pfizer) and Tularik (now Amgen). They have made significant contributions to many Structure-Based Drug Discovery (SBDD) programs and published their works in Science and Cell.

Accelagen has established a track record of success in purification of recombinant proteins in all major classes of drug targets, antibodies, GPCRs, and therapeutic proteins. Using proteins Accelagen purified, our customers have determined high resolution co-crystal structures, completed HTS campaigns, and animal studies.

State-of-the-Art Technologies

Our protein purification facility includes:

  • Multiple AKTA chromatography systems in temperature controlled environment
  • Dedicated systems for low endotoxin purification
  • Extensive selections of chromatography media
  • Flexible configuration of chromatography columns tailored to each purification
  • Vigorous QA/QC to ensure the highest quality and reproducibility

Accelagen provides comprehensive protein purification services for drug discovery and development. We specialize in purifying recombinant proteins for protein crystallography and low endotoxin proteins for toxicology studies.

Accelagen has developed protocols to purify many hard-to-purify proteins that led to successful crystallization of these proteins. We have extensive experiences in purification of many families of proteins from cell pastes, media, or membranes.

Accelagen has routinely purified low endotoxin proteins and antibodies in gram quantity. We have developed protocols for large scale endotoxin removal and successfully purified low endotoxin proteins from E. coli.

For more information on how we can increase your protein purification capacity and improve your protein purification, contact us now.


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Baculovirus/Insect Cell Protein Expression

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TurboBacTM Process

Accelagen has validated a proprietary process of recombinant baculovirus production and recombinant protein expression in insect cells. This streamlined process uses the latest technology of baculovirus expression vector system (BEVS) and disposable bioreactors. Our TurboBac process offers parallel production and evaluation of multiple recombinant baculoviruses for protein expression. The automated system monitors the viral infection process and enables consistent high quality recombinant baculovirus production and protein expression in insect cells. TurboBac process minimizes the viral amplification and shortens the timeline to large scale production. We have routinely used P1 recombinant viruses for large scale expression of over 100 liters.

Many mammalian proteins are not easily expressed in E. coli in soluble form. Identifying the soluble expression conditions in E. coli or the refolding conditions is a great challenge. Baculovirus expression vector system (BEVS) has proven to be an effective system to expression soluble functional mammalian proteins. Accelagen’s TurboBac process has eliminated many time consuming steps and provides a fast and cost-effective expression system for functional mammalian protein including GPCRs. The timeline of baculovirus expression is comparable to E. coli expression.

Accelagen has 100% success rate of generating high titer recombinant viruses.

Baculovirus Expression Services

Accelagen provides a full suite of baculovirus expression services:

  • High-titer P1 recombinant baculovirus production in 2 weeks
  • Titerless Infected-cell Preservation and Scale-up (TIPS)
  • Preparation of Baculovirus infected insect cells (BIIC)
  • Quick optimization of expression condition
  • Large-scale expression of intracellular or secreted proteins in Wave Bioreactor
  • High capacity recombinant baculovirus production to evaluate multiple constructs

When the high-titer virus is delivered, you know it expresses your protein.

When the pellets or media are delivered, you know they contain your protein.

For more information on how we can accelerate your baculovirus production and expression, contact us now.


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Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
Price on request

To many customers, Accelagen has shortened project timelines and reduced total cost of mammalian expression. Our scientists have extensive experiences in

  • production in CHO, 293, hybridoma, and many other cell lines
  • production of secreted proteins, membrane proteins, and multi-subunit complexs
  • improving protein expression in Wave bioreactors and Cell Factory
  • Production of functional GPCR

Mammalian Culture Condition Optimization

Accelagen's TurboCell OptimizationTM investigates biological properties of each cell line (CHO, 293, hybridoma etc) and systematically examines physical and biochemical parameters to achieve optimal culture conditions and improve protein yield. The optimized conditions are scalable from flasks to Wave bioreactors. The example shown in the figure demonstrates a 5-fold increase in monoclonal antibody production in hybridoma as the result of TurboCell OptimizationTM, in which the final antibody yield is increased from historical 50 mg/L to 300 mg/L.

Mammalian Expression Services

Accelagen provides a full suite of mammalian expression services:

  • large-scale mammalian expression in Wave Bioreactors
  • large-scale mammalian expression in Cell Factories
  • large-scale transient transfection and BacMam expression
  • cell line development
  • cell culture and titer improvement
  • cell banking

When the pellets or media are delivered, you know they contain your protein.

Accelagen can significantly improve mammalian expression


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Bacterial Protein Expression

Price on request

Low induction temperature is a proven method to improve soluble expression in E. coli. Accelagen has the capacity to quickly screen soluble expression conditions for multiple expression constructs. Our refrigerated shaker-incubators and high density culture system offer high capacity of E. coli expression.

  • Proprietary expression vectors for customized protein expression and purification.
  • SUMO-tag and various chaperones to improve protein solubility.
  • Routinely increased total or soluble protein expression by more than 10 fold using proprietary techniques


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Biochemistry & Molecular Biology

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Accelagen's Molecular Biology Services enables the unlimited exploration of the protein space. Through expression construct design, site-directed mutagenesis, domain swapping and gene synthesis, Accelagen can assist you to increase the possibility of soluble expression or protein crystallization.

Combined with the Protein Expression and Protein Purification services, Accelagen offers a comprehensive gene-to-protein platform. Accelagen has developed proprietary expression vectors for high level of protein expression and effective protein purification.

Gene accession numbers provide sufficient information for Accelagen to produce premium quality functional proteins. Our expanding product portfolio demonstrates our capacity to ‘turn’ accession numbers into functional proteins.

Accelagen has developed TurboLigationTM Kit for 5 minute quick ligation at room temperature.


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Antigen Expression and Purification

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Bioconjugation/Chemical Crosslinking

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PCR Product Purification

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PCR

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PCR Services


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DNA Services

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DNA Services


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Nucleic Acid Services

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Nucleic Acid Services


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Protein Production

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Protein Expression Services


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Protein Services

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Protein Services


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Biology

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Biology Services


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Antibody Services

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Antibody Services


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Recombinant Protein Expression

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Antibody Purification

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Chun Luo

CEO

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