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Absorption Systems

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Exton, Pennsylvania, US

About Absorption Systems

Founded: 1996 Type: Privately Held Size: 51-200 employees

Absorption Systems is a preclinical CRO that develops, and implements research tools that allow our customers to better predict human outcomes. We have locations in Exton, PA, San Diego, CA, and Panama City, Panama. We work with a wide variety of customers in the pharmaceutical and biotech industry, including... Show more »

Absorption Systems is a preclinical CRO that develops, and implements research tools that allow our customers to better predict human outcomes. We have locations in Exton, PA, San Diego, CA, and Panama City, Panama. We work with a wide variety of customers in the pharmaceutical and biotech industry, including small virtual pharma companies, large pharma companies with weekly programs, and specialty CROs looking for services that complement their own offerings. Our flexibility allows us to adapt to the needs of each customer.

We believe that projects should be viewed as a collaboration, which allows for us to work with you to design studies and develop custom models when necessary. This collaborative approach has resulted in the generation of several innovative models to aid in drug discovery and development, through working partnerships within the industry and with regulatory agencies.

Absorption Systems has bicoastal facilities located in Exton, Pennsylvania and San Diego, California, as well as sales offices in Germany to better serve customers across the globe. The Exton-based headquarters, just outside of Philadelphia, have approximately 30,000 sq. ft. of space. The facility encompasses executive and sales offices, in vitro labs, and a small animal vivarium. Nearly all of Absorption Systems’ in vitro work is performed here, including permeability, metabolism, drug transporters, formulations, and physicochemical properties, plus in vivo rodent PK. Bioanalysis services are provided with the help of 21 LC-MS/MS machines and an LTQ Orbitrap XL. The facility is also able to handle radioactive compounds and has a license from the DEA to handle compounds classified as high as Schedule I.

Our San Diego, California facility is AAALAC-accredited, USDA-registered, OLAW-assured, and GLP-compliant. The animal vivarium comprises nearly 30,000 sq. ft. and provides the setting for Absorption Systems’ in vivo services. This facility has the space to house animals of all sizes from rats and mice up to dogs and swine and is capable of performing a large range of preclinical services including toxicology, oncology, surgery, pharmacokinetic, and pharmacodynamic studies. In addition to a comprehensive service offering for small and large molecule drug development, Absorption Systems also offers customized services to support the development of biomaterials and medical devices. The state-of-the-art 1,100 sq. ft. surgical suite includes a separate observation room, fully equipped catheterization lab, and conference room space.

Recent Publications

  • Qing Wang, Robert Strab, Paula Kardos, Chrissa Ferguson, Jibin Li, Albert Owen and Ismael J Hidalgo, Application and limitation of inhibitors in drug-transporter interactions studies. International Journal of Pharmaceutics, 356: 12-8 (2008).
  • Belinsky MG, Guo P, Lee K, Zhou F, Kotova E, Grinberg A, Westphal H, Shchaveleva I, Klein-Szanto A, Gallo JM, Kruh GD. Multidrug resistance protein 4 protects bone marrow, thymus, spleen, and intestine from nucleotide analogue-induced damage. Cancer Res. 2007 Jan 1;67 (1):262-8.
  • Tanja Obradovic, Glenn L. Dobson, Tomotaka Shingaki, Thomas Kungu, and Ismael J. Hidalgo, Assessment of the First and second generation antihistamines brain penetration and role of P-glycoprotein, Pharm. Res. 24: 318-327 (2006).
  • Qing Wang, Joseph D. Rager, Kate Weinstein, Paula S. Kardos, Glenn Dobson, Jibin Li, and Ismael J. Hidalgo, Evaluation of the MDR-MDCK cell line as a Permeability Screen for the Blood-Brain Barrier, International Journal of Pharmaceutics, 288: 349-359 (2005).
  • Wang Q, Rager JD, Weinstein K, Kardos PS, Dobson GL, Li J, Hidalgo IJ, “Evaluation of the MDR-MDCK cell line as a permeability screen for the blood-brain barrier”, Int J Pharm. 2005 Jan 20;288(2):349-59.
  • Ponnappa BC, Israel Y, Aini M, Zhou F, Russ R, Cao QN, Hu Y, Rubin R. Inhibition of tumor necrosis factor alpha secretion and prevention of liver injury in ethanol-fed rats by antisense oligonucleotides. Biochem Pharmacol. 2005 Feb 15;69(4):569-77.
  • Gallo JM, Vicini P, Orlansky A, Li S, Zhou F, Ma J, Pulfer S, Bookman MA, Guo P. Pharmacokinetic model-predicted anticancer drug concentrations in human tumors. Clin Cancer Res. 2004 Dec 1;10(23):8048-58.
  • M. Garcia, J. Rager, Q. Wang, R. Strab, I. Hidalgo, A. Owen and J. Li “Cryopreserved Human Hepatocytes as Alternative In Vitro Model for Cytochrome P450 Induction Studies”, In Vitro Cell. Dev. Biol. – Animal, 39, 283-287, 2003.
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Our Services (101)


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Ocular Toxicology

Price on request

Our formulations team can help identify and prepare dose vehicles that are appropriate for short or long-term toxicology studies. Dosing and observations are handled by our expert staff with more than a decade’s experience in small and large animal studies (acute, subchronic, chronic, dose range finding, etc). Our in-house... Show more »

Our formulations team can help identify and prepare dose vehicles that are appropriate for short or long-term toxicology studies. Dosing and observations are handled by our expert staff with more than a decade’s experience in small and large animal studies (acute, subchronic, chronic, dose range finding, etc). Our in-house bioanlaytical capabilities (both non-GLP and GLP) enable us to oversee your project from beginning to end.
Services available:
Direct / Indirect Ophthalmoscopy
Slit-Lamp Biomicroscopy
Optical Coherence Tomography (OCT)
Fundus Photography
Fluorescein Angiography
Auto-Fluorescence
Indocyanine Green (ICG)
Pachymetry
Intraocular Pressure
Electroretinography (ERG, mfERG), VEP
Specular Microscopy
Ultrasound
Phacoemulsification
Vitrectomy
Cell Culture Room

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Medical Device Testing

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Absorption Systems offers a wide range of in vivo preclinical medical device testing services at our San Diego facility. Our goal is helping our clients attain successful 510(k) or PMA submissions. Our USDA-registered and NIH-assured, AAALAC-accredited GLP facility is also available for contract vivarium leasing and physician... Show more »

Absorption Systems offers a wide range of in vivo preclinical medical device testing services at our San Diego facility. Our goal is helping our clients attain successful 510(k) or PMA submissions. Our USDA-registered and NIH-assured, AAALAC-accredited GLP facility is also available for contract vivarium leasing and physician training sessions.

Overview of Services
In vivo, GLP and non-GLP
In-House IACUC
Research Protocols
Model Development
Feasibility Studies
Safety and Efficacy
Physician Training & Cadaver Studies
Histopathological Support
Bioanalytical Support
Vivarium Leasing
Surgical Suites

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Human Rabbit Guinea pig Sheep Goat Mouse Pig Rat Dog Minipig Hematology Cardiovascular Ophthalmology Metabolism and Endocrinology Musculoskeletal/Orthopedic Echocardiography Clinical chemistry Fluoroscopic Radiography Ultrasound & TEE Endoscope Blood Gas Measurement MRI CT & Micro-CT Biomarkers Show 24 more tags Show less

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In vivo Bioavailability/Bioequivalence Studies

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Toxicology

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You can benefit from Absorption Systems’ integrated services for toxicology support. Preclinical toxicology and related safety studies are a pivotal part of the drug development life cycle, as they are needed to demonstrate that the test compound is safe, and at what doses, for administration to humans as part of clinical... Show more »

You can benefit from Absorption Systems’ integrated services for toxicology support. Preclinical toxicology and related safety studies are a pivotal part of the drug development life cycle, as they are needed to demonstrate that the test compound is safe, and at what doses, for administration to humans as part of clinical trials.
Dosing and observations are handled by our expert staff with more than a decade’s experience in small and large animal studies (acute, subchronic, chronic, dose range finding, etc). Our in-house bioanlaytical capabilities (both non-GLP and GLP) enable us to oversee your project from beginning to end.

Dose Routes

  • Intravenous
  • Oral
  • Intraperitoneal
  • Sublingual
  • Buccal
  • Transdermal
  • Intraduodenal
  • Subcutaneous
  • Intracolonic
  • Intramuscular
  • Intratracheal
  • Ocular

Dose Vehicle Development & Evaluation:

  • Excipient Selection
  • API – Compatibility
  • Vehicle – Suitability
  • Stability (dose vehicle)
  • Artificial Fluids Interactions
  • Simulated (gastric and intestinal fluids)
  • Phosphate-buffered Saline
  • Albumin Solution
  • In Vivo Performance Characterization
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In vivo Percutaneous Absorption Studies

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We offer various surgically ported animal models to help with formulation assessment in addition to the standard rodent, canine and non-human primate models for oral and intravenous dosing. These include rats, dogs, and mini-pigs fitted with intestinal cannulas implanted at various sites for dosing solutions or suspensions, as... Show more »

We offer various surgically ported animal models to help with formulation assessment in addition to the standard rodent, canine and non-human primate models for oral and intravenous dosing. These include rats, dogs, and mini-pigs fitted with intestinal cannulas implanted at various sites for dosing solutions or suspensions, as well as dogs fitted with duodenal fistulas for dosing solids, semi-solids, capsules, and tablets directly into the duodenum. Several of these animal models are available routinely and other custom models can be prepared in line with customer needs.

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Animal Tissue Procurement

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CYP Inhibition Assay

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We incubate the test article, at either a single concentration or 7 concentrations, with a selective probe substrate and human liver microsomes. We monitor a specific metabolite and compare its rate of appearance with that of a solvent control to assess the extent of inhibition of a given CYP by each concentration of a test... Show more »

We incubate the test article, at either a single concentration or 7 concentrations, with a selective probe substrate and human liver microsomes. We monitor a specific metabolite and compare its rate of appearance with that of a solvent control to assess the extent of inhibition of a given CYP by each concentration of a test article. An IC50 can be determined when 7 concentrations are assessed. For reversible inhibition, an IC50 ≤ the plasma Cmax translates to a likely clinical DDI.
Time-dependent inhibition (TDI) is evaluated by quantifying the IC50 shift after pre-incubation of test article with liver microsomes +/- NADPH. Clinical correlation for a positive TDI score can then be determined by a follow-up in vitro measurement of KI and kinact. Comparison of KI and kinact to plasma Cmax can predict potential clinical DDIs due to TDI.

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Metabolite Identification

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We can perform isolation of a targeted metabolite from various in vitro and in vivo matrices followed by unambiguous structural elucidation using NMR. If isolation of metabolites is not feasible from biological matrices, we offer a complimentary approach – biomimetic oxidation.

Two-Tiered Approach

Preliminary

*... Show more »

We can perform isolation of a targeted metabolite from various in vitro and in vivo matrices followed by unambiguous structural elucidation using NMR. If isolation of metabolites is not feasible from biological matrices, we offer a complimentary approach – biomimetic oxidation.

Two-Tiered Approach

Preliminary

  • Report Expected Metabolites
    Comprehensive Analysis
  • Thorough metabolite detection
  • Structure Elucidation
    -Soft spot prioritization
  • Relative Abundance
  • Isolation of targeted metabolite(s)
    -Various matrices
    -Unambiguous structural elucidation using NMR
  • Biomimetic Oxidation
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Animal Bone Marrow

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Canine bone marrow collection

Canine bone marrow collection

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Plasma Protein Binding

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Plasma protein binding is critical in the early phases of drug development to determine the amount of a drug available for distribution throughout various organs and tissues in the body. In addition, these in vitro tests can predict the extent to which the drug will be bound to plasma proteins in vivo.

Plasma protein binding is critical in the early phases of drug development to determine the amount of a drug available for distribution throughout various organs and tissues in the body. In addition, these in vitro tests can predict the extent to which the drug will be bound to plasma proteins in vivo.

  • Low Protein Binding → High Free Drug Distribution
  • High Protein Binding → Low Free Drug Distribution
  • Protein bound drugs may have greater difficulty crossing cell membranes
  • Red Blood Cell / Plasma Partitioning
  • Multiple Species
  • Multiple Platform
    -Ultrafiltration
    - Equilibrium Dialysis
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Solubility and Dissolution Testing

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  • LogD by Shake Flask
  • Solubility by Shake Flask
  • Stability in Buffer
  • Stability in Gastric Fluids (FeSSIF, FaSSIF, SGF)
  • Stability in Plasma/Whole Blood
  • LogD by Shake Flask
  • Solubility by Shake Flask
  • Stability in Buffer
  • Stability in Gastric Fluids (FeSSIF, FaSSIF, SGF)
  • Stability in Plasma/Whole Blood
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Product Stability Testing

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ADME/DMPK Studies

Drug Metabolism and Pharmacokinetics
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We offer an extensive range of preclinical animal models for drug discovery and development, both at its own sites and through access to preferred providers.

Pre-approved protocols for:

  • Intravenous
  • Intraperitoneal
  • Buccal
  • Intraperitoneal
  • Ocular
  • Oral
  • Sublingual
  • Topical
  • Subcutaneous
  • Intracheal

We offer an extensive range of preclinical animal models for drug discovery and development, both at its own sites and through access to preferred providers.

Pre-approved protocols for:

  • Intravenous
  • Intraperitoneal
  • Buccal
  • Intraperitoneal
  • Ocular
  • Oral
  • Sublingual
  • Topical
  • Subcutaneous
  • Intracheal
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RNAi Technology Development

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Contract Services Directory

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Radiography

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Preclinical Study Design

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Animal Model in vivo Analyses

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Nonclinical Research

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In vitro Metabolic Stability Assays

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Mass Spectrometry

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Hit to Lead and Lead Optimization

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High Throughput Screening (HTS)

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Pharmaceutical Formulation

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Feasibility Studies

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Drug Development

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Contract Research

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In vitro Percutaneous Absorption Studies

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The In Vitro Dissolution Absorption System (IDASTM) combines traditional dissolution testing with a means to determine and quantify interactions with a bio-relevant membrane. IDAS provides the ability to evaluate absorption, permeation, accumulation, biomarker regulation and metabolism, as well as the ability to test a... Show more »

The In Vitro Dissolution Absorption System (IDASTM) combines traditional dissolution testing with a means to determine and quantify interactions with a bio-relevant membrane. IDAS provides the ability to evaluate absorption, permeation, accumulation, biomarker regulation and metabolism, as well as the ability to test a finished dosage form from a tablet, capsule to suspension. The characterized and validated system allows for multiple dissolution media and drug products from all BCS classes.

Applications:

Evaluate Formulations – Bioequivalence, Post Approval Changes, 505(b)(2) Formulations
Evaluate Food Effects
Evaluate Locally Acting GI Products

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Biopharmaceutical Development

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Biopharmaceutical Process Development

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Biological Testing

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Bioanalytical Analysis LC/MS/MS

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Animal Model Housing and Maintenance

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Assay Development

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Analytical Chemistry Services

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Time Dependent Study

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Solute-Linked Carrier (SLC) Transporter Inhibition Assays

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UDP-Glucuronosyltransferases (UGT) Assays

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Nucleoside Transporter Assays

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Glucose Transporter Assays

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Organic Ion Transporter Assays

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Peptide Transporter Assays

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Amino Acid Transporter Assays

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iTRAQ Protein Labeling

Isobaric Tags for Relative and Absolute Quantitation
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CYP Induction Assay

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Cyp Induction (1A2,2B6,3A4) EA409

Freshly isolated or cryopreserved hepatocytes are incubated with test article for 3 days.

Enzyme activity approach: Hepatocytes are then incubated with a selective probe substrate. Appearance of metabolite is compared to that of a control treatment (no test article) via LC-MS/MS in order to... Show more »

Cyp Induction (1A2,2B6,3A4) EA409

Freshly isolated or cryopreserved hepatocytes are incubated with test article for 3 days.

Enzyme activity approach: Hepatocytes are then incubated with a selective probe substrate. Appearance of metabolite is compared to that of a control treatment (no test article) via LC-MS/MS in order to assess the ability of the test article to induce a given CYP’s activity.
mRNA approach: mRNA is then isolated from the hepatocytes. cDNA is synthesized and used for qPCR to measure fold induction of CYP-specific mRNA. Comparison of treated cells to a control treatment (no test article) gives the article’s CYP induction potential.

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Ocular Absorption Studies

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Vaginal Permeability Studies

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HT ADME

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Histopathological Examination

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Protein Binding Ultrafiltration

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Small Molecule Bioanalysis

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In vivo Protein Binding Assays

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High Resolution Mass Spectrometry

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In vitro Bioavailability/Bioequivalence Studies

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In vivo Drug Efficacy Testing

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Accelerated Stability Testing

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Microsomal Stability Assay

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Chemistry and Materials

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Specialty Mass Spectrometry Methods

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Enzyme Assays

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Product Development, Testing, and Packaging

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Cell-Based Assays

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Clinical Research

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Clinical Laboratory Services

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Bioanalytical Assays

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Waters Xevo TQ-S
Thermo Scientific LTQ Orbitrap XL
AB Sciex 4000 QTRAP
AB Sciex API 4000
Waters Acquity UPLC
Leap CTC Analytics Autosamplers
Tomtec Quadra 96 robotic sample prep workstation
Conventional HPLC with UV detection
Fluorescence detectors
Scintillation counters

Waters Xevo TQ-S
Thermo Scientific LTQ Orbitrap XL
AB Sciex 4000 QTRAP
AB Sciex API 4000
Waters Acquity UPLC
Leap CTC Analytics Autosamplers
Tomtec Quadra 96 robotic sample prep workstation
Conventional HPLC with UV detection
Fluorescence detectors
Scintillation counters

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In vivo Toxicity Testing

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Safety Pharmacology

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Project Management

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Omics

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Animal Models and Studies

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Biology

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Biomolecular Interaction Analysis

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Pharmacology

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Pharmacology Services

Pharmacology Services

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Imaging & Spectroscopy

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Cells and Tissues

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Biochemical Assays

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Transporter & Cell Uptake Assays

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Bioanalysis

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Lead Identification and Validation

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Functional Human Tissue Assays

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Nucleic Acid Services

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Hit Identification

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Animal Biospecimens

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In vivo PK/PD Studies

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RNA Services

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Transferase Assays

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Manufacturing Services

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Spectroscopy

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In vitro ADME/DMPK Studies

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Proteomics

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Pharmacology & Toxicology

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In vitro Disease Models

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Drug Discovery

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Biospecimens

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Formulation Services

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Drug Discovery & Development

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Medical Devices & Diagnostics

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Medical Devices & Diagnostics Services

Medical Devices & Diagnostics Services

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Business Development Consulting

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Drug Absorption Studies

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Drug Absorption Studies Services

Drug Absorption Studies Services

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Biochemistry & Molecular Biology

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Metabolomics

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Metabolomics Services

Metabolomics Services

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  • Positive review for Tissue Processing:

    November 2018

    "The experience was overall positive. The platform was very easy to navigate."

Absorption Systems has not received any endorsements.