Absolute Antibody

At Absolute Antibody we have developed a proprietary cloning system to enable rapid reformatting of antibodies into almost any format. Our antibody engineering services include chimerization, humanization, isotype switching and the manufacturing of recombinant antibody fragments and bispecific antibodies.

Species, Isotype and Subtype switching
Change the constant domain of your antibody to Human, Mouse, Rabbit, Rat, Hamster, Goat, Sheep, Pig, Horse, Dog, Chicken, Fish, Platypus or a whole range on non-human Primates. Moreover, our in-house experts can determine antibody sequences from most species genomes (even if only partial assemblies are available).
Change the isotype or subtype of any antibody to alter the in vivo effector function, stability and aggregation profile of an antibody. Converting an IgG to an IgM can be used to increase avidity

Humanization
Royalty free antibody humanization service to identify a variant that retains activity and has preferential manufacturing criteria (e.g. high expression, low aggregation).

Antibody fragments
Convert an antibody into a fragment, such as a single chain Fv (scFv), Fab or F(ab)’2, followed by expression and purification at any scale.

Bispecific and multispecific antibodies
Bispecific/multispecific antibody service utilising a variety of formats.

Absolute Antibody offers transient mammalian expression and purification of your antibody at any scale from 1 microgram to 1 gram. Our proprietary high yielding serum-free mammalian production system - ABS293 is derived from HEK293 cells and allows to offer a service which is:

• Rapid – from electronic sequence of antibody to purified protein in as little as 6 weeks. Even quicker for repeat batches where the antibody is already cloned into a vector.
• High purity – no albumin or bovine IgG contamination
• Low endotoxin – compatible with all in vitro or in vivo applications
• Flexible – almost all species, IgG-subtypes and alternative isotypes and formats are available (see antibody enginering service for more information).

We also offer a guaranteed IgG expression service and a variant expression service

Next Generation Sequencing of Hybridomas (NGS)

Hybridoma sequencing is the sequencing of the cDNA encoding the variable heavy (VH) and variable light (VL) domains of your hybridoma cell line. Sequencing the antibody genes of your hybridoma offers many advantages including the following:

• Security - your antibody can always be produced recombinantly even if your hybridoma line is lost
• Humanization - once you know the VH and VL sequences you can proceed to humanisation if part of a therapeutic antibody project
• Flexibility - convert your antibody to any species, isotype or format
• Intellectual property - patent the sequences of your unique variable domains

Absolute Antibody offers a guaranteed antibody sequencing service.

We offer a royalty free antibody humanization service which aims to identify a variant that retains activity and has preferential manufacturing criteria (e.g. high expression, low aggregation).