Absolute Antibody specialises in the sequencing, engineering, and recombinant production of antibodies. We offer antibody sequencing from cell lines such as hybridomas or immortalised B-cells as well as sequencing from purified antibody protein. Our engineering services include species/isotype/subtype switching, full humanization, generation of custom bispecifics, scFv, antibody fragments etc. If you can describe it, we can make it.
Using our serum-free transient expression platform we cater for milligram to gram scales with delivery in as little as 3 weeks. All of our services are royalty free.
We also have a catalog of recombinant reagents, including antibodies against common immune checkpoints and a range of research-grade biosimilars. All are available in any species and any isotype to suit your assay.
We're always excited to hear about new ideas, exciting innovations, and potential collaborations. Challenge us with your antibody problems - we'll see what we can do.
Standard sample shipment requirements:
Standard shipment on ice packs, dry ice on request
At Absolute Antibody we have developed a proprietary cloning system to enable rapid reformatting of antibodies into almost any format. Our antibody engineering services include chimerization, humanization, isotype switching and the manufacturing of recombinant antibody fragments and bispecific antibodies.
Species, Isotype and Subtype switching
Change the constant domain of your antibody to Human, Mouse, Rabbit, Rat, Hamster, Goat, Sheep, Pig, Horse, Dog, Chicken, Fish, Platypus or a whole range on non-human Primates. Moreover, our in-house experts can determine antibody sequences from most species genomes (even if only partial assemblies are available).
Change the isotype or subtype of any antibody to alter the in vivo effector function, stability and aggregation profile of an antibody. Converting an IgG to an IgM can be used to increase avidity
Royalty free antibody humanization service to identify a variant that retains activity and has preferential manufacturing criteria (e.g. high expression, low aggregation).
Convert an antibody into a fragment, such as a single chain Fv (scFv), Fab or F(ab)’2, followed by expression and purification at any scale.
Bispecific and multispecific antibodies
Bispecific/multispecific antibody service utilising a variety of formats.
Absolute Antibody offers transient mammalian expression and purification of your antibody at any scale from 1 microgram to 1 gram. Our proprietary high yielding serum-free mammalian production system - ABS293 is derived from HEK293 cells and allows to offer a service which is:
We also offer a guaranteed IgG expression service and a variant expression service
Next Generation Sequencing of Hybridomas (NGS)
Hybridoma sequencing is the sequencing of the cDNA encoding the variable heavy (VH) and variable light (VL) domains of your hybridoma cell line. Sequencing the antibody genes of your hybridoma offers many advantages including the following:
Absolute Antibody offers a guaranteed antibody sequencing service.
We offer a royalty free antibody humanization service which aims to identify a variant that retains activity and has preferential manufacturing criteria (e.g. high expression, low aggregation).
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