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AAPPTec

Louisville, Kentucky, US

AAPPTec LLC, located in Louisville, KY, USA, is the only company that provides a complete line of peptide products ranging from automated peptide synthesizers to related laboratory equipment and high quality reagents designed to assist chemists in preparing and analyzing high quality peptides on research to semi-preparative and preparative scale.

In addition, AAPPTec provides all the technical information and instructions for each step of peptide synthesis and purification.

For those who prefer not to synthesize their own peptides, AAPPTec offers custom peptides in quantities from mg to kg, in purities from crude to 98% pure, all at an affordable price.

Founded by renowned peptide chemist, Dr. Hossain Saneii, and staffed by peptide chemists and engineers with a combined 100+ years of experience in the peptide field, AAPPTec understands what is important to peptide chemists and to their success.

AAPPTec... Show more »

AAPPTec LLC, located in Louisville, KY, USA, is the only company that provides a complete line of peptide products ranging from automated peptide synthesizers to related laboratory equipment and high quality reagents designed to assist chemists in preparing and analyzing high quality peptides on research to semi-preparative and preparative scale.

In addition, AAPPTec provides all the technical information and instructions for each step of peptide synthesis and purification.

For those who prefer not to synthesize their own peptides, AAPPTec offers custom peptides in quantities from mg to kg, in purities from crude to 98% pure, all at an affordable price.

Founded by renowned peptide chemist, Dr. Hossain Saneii, and staffed by peptide chemists and engineers with a combined 100+ years of experience in the peptide field, AAPPTec understands what is important to peptide chemists and to their success.

AAPPTec continually strives to provide the latest technology at the most competitive prices and the highest level of customer support to peptide chemists worldwide.

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Peptide Synthesis
Price on request

Custom peptide synthesis provided by AAPPTec is available in all scales, from milligram research scale to multi-kilogram production scale. AAPPTec custom peptides are produced in a timely manner at competitive prices. AAPPTec uses top quality resins, reagents, and state-of-the-art methods in each custom peptide synthesis to assure... Show more »

Custom peptide synthesis provided by AAPPTec is available in all scales, from milligram research scale to multi-kilogram production scale. AAPPTec custom peptides are produced in a timely manner at competitive prices. AAPPTec uses top quality resins, reagents, and state-of-the-art methods in each custom peptide synthesis to assure the highest yield and purity. Custom peptides containing non-standard amino acids, modified side chains or other modifications are synthesized to the same high quality standards as standard peptides. All custom peptide products are provided with a complete quality control package, including HPLC and Mass Spectral Analysis, to confirm the purity and identity of the peptide. From 2 amino acids to 85 amino acids, AAPPTec can prepare peptides in the scale and purity required. We comply with the most stringent quality control specifications for a reasonable price.

Custom Peptide Synthesis

AAPPTec chemists utilize state-of-the-art equipment and the latest peptide synthesis techniques to quickly prepare high quality custom peptides at competitive prices. AAPPTec chemists have the experience and expertise to prepare long peptides in high purity. As an example, AAPPTec has synthesized and delivered 78 to 81 amino acid custom peptides at 95% purity.

Custom Peptide Purity

AAPPTec provides custom peptides in various purity levels. Higher purity peptides incur greater costs due to additional purification. The purity level required for a peptide depends on its application. Peptides for epitope mapping and polyclonal antibody production require moderate purity levels. Peptides utilized in tissue culture or non-quantitiative experiments are typically 80% pure or better. Peptides for bioassays and in vivo studies are usually 90% pure or better. Peptides used as chromatography standards need to be 98% pure or better, while 95% purity is sufficient for most other peptide applications.

AAPPTec custom peptides are provided with the following purities:

  • Immunological Grade (suitable for forming polyclonal antibodies)
  • 80% or greater (tissue culture; ligand for affinity purification; non-quantitative antibody blocking experiments)
  • 90% or greater (in vivo studies; bioassays; markers for electrophoresis; monoclonal antibodies)
  • 95% or greater (ELISA; RIA; enzyme substrate)
  • 98% (NMR; chromatography standards)

AAPPTec custom peptide synthesis services include:

  • MAP Peptides
  • Cyclic Peptides
  • Peptides Containing Unusual Amino Acids
  • Peptides with Modified Side Chains
  • Peptide Library Synthesis

AAPPTec custom peptide synthesis services can prepare custom peptides with the following modifications:

  • D-Amino Acids (Custom peptides may contain one or more D-amino acids)
  • N-Methyl Amino Acids (Peptides with N-methylation of the peptide backbone)
  • Phosphorylation (Ser, Thr, Tyr)
  • Sulfonation: Tyr(SO3H) (sulfonated peptides)
  • Isotopic Amino Acids (C13 and/or N15 labeling)
  • N-Terminal Peptide Modifications
  • C-Terminal Peptide Modifications
  • Fluorescence/Dye Labeling

Additional Services

AAPPTec can provide the following services for an additional fee:

  • Amino Acid Analysis
  • Aliquoiting
  • TFA Removal

Custom Peptide Prices:

AAPPTec custom peptide prices are as low as $4.60 per amino acid for simple peptides under 30 amino acids. Peptide prices will depend on the purity and quantitity of peptide required. Peptides containing 30 amino acids or more and peptides with modifications require special quotations.

Large Scale Custom Peptide Production

AAPPTec can perform custom peptide synthesis in larger scale, from gram to multi-kilogram quantities. AAPPTec complies with the most stringent quality control specifications at a competitive price. Let our experienced chemists assist with your development and production needs. All requests are evaluated with strict confidentiality.

For our profound experience in custom peptide synthesis, well-trained technical team and highest production standards, we hope to be your strong and reliable partner in the life sciences industry.

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Chemical Reagents
Price on request

AAPPTec offers a complete selection of coupling and deprotection reagents to support the wide range of chemistry protocols available. We provide these products in quantities suitable for both production and research scales.

AAPPTec offers a complete selection of coupling and deprotection reagents to support the wide range of chemistry protocols available. We provide these products in quantities suitable for both production and research scales.

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Peptide Libraries
Price on request

AAPPTec custom peptide synthesis services can provide custom peptide libraries for drug discovery, high-throughput screening and SAR studies, including alanine scans and truncation sets. Peptide libraries are provided in 96-well plates with approximately 2 µmol of lyophilized peptide in each well.

AAPPTec utilizes optimized... Show more »

AAPPTec custom peptide synthesis services can provide custom peptide libraries for drug discovery, high-throughput screening and SAR studies, including alanine scans and truncation sets. Peptide libraries are provided in 96-well plates with approximately 2 µmol of lyophilized peptide in each well.

AAPPTec utilizes optimized protocols and chemistries in custom peptide synthesis to ensure consistent purity, quantity and quality, library-to-library and within each library.

Custom peptide library modifications include D-amino acids, unusual amino acids, biotin or fluorescent labeling, N-acylation or C-terminal amide.

Provide AAPPTec with the peptide sequences and we will prepare a quotation for your library. Custom peptide synthesis of peptide libraries typically is completed in 2-3 weeks. Typical delivery consists of lyophilized peptides in 96-well titer plates, peptide location table, and MS and HPLC data.

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Monoclonal Antibody (mAb) Production
Price on request

Antigen Preparation

  • Peptide design
  • Peptide synthesis
  • Peptide conjugation
  • Protein expression

Custom Monoclonal Antibodies

  • Application guaranteed antibody (ELISA, Western blot, IHC)
  • Modification-specific antibody (phosphorylated, methylated, acetylated)

Antibody Purification

**Antibody... Show more »

Antigen Preparation

  • Peptide design
  • Peptide synthesis
  • Peptide conjugation
  • Protein expression

Custom Monoclonal Antibodies

  • Application guaranteed antibody (ELISA, Western blot, IHC)
  • Modification-specific antibody (phosphorylated, methylated, acetylated)

Antibody Purification

Antibody Identification

  • ELISA
  • Dot blot
  • Western blot
  • Immunohistochemistry (IHC)

Antibody Labeling

  • Horseradish peroxidase (HRP)
  • Biotin

AAPPTec offers a variety of monoclonal antibody production packages. These packages cover all antibody production steps including antigen preparation, immunization, sera collection and antibody identification.

Custom Monoclonal Antibody Production Services

Peptide Services Time
Peptide Design 1-2 days
Peptide Synthesis 2 weeks
Peptide Conjugation (KLB/BSA/OVA, 5 mg or less) 2-3 days
Protein Services Time
Gene Synthesis 3-4 weeks
Gene Amplification (Template provided by customer, 1000 bp or less) 2-3 weeks
Vector Construction 2-3 weeks
Recombinant Selection 1 week
Protein Expression and Purification 2-3 weeks
Monoclonal Antibody Services Time
Protein Checked by SDS-PAGE 2-3 days
Pre-immune Sera Collection 1 day
Immunization and Sera Collection (4 mice) 6-10 days
ELISA Identification 2-3 days
Fusion and Hybridoma Developmen 10-16 weeks
Isotype Determination 1 week
Ascites Production (3 mice) 3-4 weeks
Roller Bottle Cell Culture (1 L) 5-7 weeks
Prescreen (ELISA) 2-3 days
Prescreen (Western blot) 1 week
Prescreen (Dot blot) 1 week
Clone Maintenance (1 clone) 1 week
Clone Storage (1 clone) 1 week
Antibody Purification Services Time
Saturated Ammonium Sulfate Precipitation (60 mL or less) 3-5 days
Protein A/G Affinity Purification (60 mL or less) 3-5 days
Antigen Affinity Column Preparation 2-3 days
Antigen Affinity Purification 3-5 days
Antibody Identification Services Time
ELISA Identification (1-10 samples) 2-3 days
Cell Extraction (1 sample) 2-3 days
Tissue Extraction (1 sample) 2-3 days
Protein Concentration Assay (1 sample) 2-3 days
Western blot Identification (1-8 samples) 1 week
Dot blot Identification (1-10 samples) 2-3 days
IHC Identification (section provided by customer, 1 photo) 1 week
Antibody Labeling Services Time
HRP Labeling (5 mg or less) 1-2 weeks
Biotin Labeling (5 mg or less) 1-2 weeks

Monoclonal Antibody Production Packages

AAPPTec offers the following packages for custom monoclonal antibody producting. The times list are average times for delivery. Actual times will vary depending on the difficulty of producing the monoclonal antibodies.

Package Options Service Items Delivery Time
Monoclonal Package 1 (From protein to ascites purification) Protein checked by SDS-PAGE, Immunization and sera collection, ELISA identification, Fusion and hybridoma development, Isotype determination, Ascites production and purification (2 clones) 50 μl Pre-immune sera (1:10 dilution), 2-5 clones, 2-5 mg Purified antibodies (2 clones), ELISA result, Isotype determination result, Project report 18-24 weeks
Monoclonal Package 2 (From peptide design to ascites purification) Peptide design and synthesis (12-22aa, 85%), Peptide conjugation, Immunization and sera collection, ELISA identification, Fusion and hybridoma development, Isotype determination, Ascites production and purification (2 clones) 2-3 mg Peptides, 50μl Pre-immune sera (1:10 dilution), 2-5 Clones, 2-5mg Purified antibodies (2 clones), ELISA result, Isotype determination result, Project report 20-26 weeks
Monoclonal Package 3 (From plasmid to ascites purification) Protein expression and purification, Immunization and sera collection, ELISA identification, Fusion and hybridoma development, Isotype determination, Ascites production and purification (2 clones) 1 mg Protein, 50 μl Pre-immune sera (1:10 dilution), 2-5 clones, 2-5 mg Purified antibodies (2 clones), ELISA result, Isotype determination result, Project report 20-26 weeks
Monoclonal Package 4 (From cDNA to ascites purification) Gene amplification, Vector construction, Protein expression and purification, Immunization and sera collection, ELISA identification, Fusion and hybridoma development, Isotype determination, Ascites production and purification (2 clones) Recombinant vector, 1 mg Protein, 50 μl Pre-immune sera (1:10 dilution), 2-5 clones, 2-5 mg Purified antibodies (2 clones), ELISA result, Isotype determination result, Project report 22-26 weeks
Monoclonal Package 5 (Phospho-specific monoclonal antibodies) Peptide design and synthesis (12-22aa, 85%), Peptide conjugation, Immunization and sera collection, ELISA identification, Fusion and hybridoma development, Isotype determination, Ascites production and purification (2 clones) 2-3 mg Peptides (unmodified and phospho), 50 μl Pre-immune sera (1:10 dilution), 2-5 Clones, 2-5 mg Purified antibodies (2 clones), ELISA result, Isotype determination result, Project report 21-26 weeks
Monoclonal Package 6 (Western guaranteed monoclonal antibodies) Peptide design and synthesis (12-22aa, 85%), Peptide conjugation, Immunization and sera collection, ELISA and Western blot identification, Fusion and hybridoma development, Isotype determination, Ascites production and purification (2 clones) 2-3 mg Peptides (unmodified and phospho), 50 μl Pre-immune sera (1:10 dilution), 2-5 Clones, 2-5 mg Purified antibodies (2 clones), ELISA and Western blot results, Isotype determination result, Project report 20-26 weeks
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Polyclonal Antibody Production
Price on request

Antigen Preparation (Polyclonal Antibody Production)

  • Peptide design
  • Peptide synthesis
  • Peptide conjugation
  • Protein expression
  • Custom Polyclonal Antibodies
  • Application guaranteed antibody (ELISA, Western blot, IHC)
  • Modification-specific antibody (phosphorylated, methylated, acetylated)

**Antibody... Show more »

Antigen Preparation (Polyclonal Antibody Production)

  • Peptide design
  • Peptide synthesis
  • Peptide conjugation
  • Protein expression
  • Custom Polyclonal Antibodies
  • Application guaranteed antibody (ELISA, Western blot, IHC)
  • Modification-specific antibody (phosphorylated, methylated, acetylated)

Antibody Purification (Polyclonal Antibodies)

Antibody Identification (Polyclonal Antibodies)

  • ELISA
  • Dot blot
  • Western blot
  • Immunohistochemistry (IHC)

Antibody Labeling (Polyclonal Antibodies)

  • Horseradish peroxidase (HRP)
  • Biotin

AAPPTec offers a variety of polyclonal antibody production packages. These packages cover all antibody production steps including antigen preparation, immunization, sera collection and antibody identification.

Custom Polyclonal Antibody Production Services

Peptide Services Time
Peptide Design 1-2 days
Peptide Synthesis 2 weeks
Peptide Conjugation (KLB/BSA/OVA, 5 mg or less) 2-3 days
Protein Services Time
Gene Synthesis 3-4 weeks
Gene Amplification (Template provided by customer, 1000 bp or less) 2-3 weeks
Vector Construction 2-3 weeks

Recombinant Selection 1 week
Protein Expression and Purification | 2-3 weeks

Polyclonal Antibody Services Time
Protein Checked by SDS-PAGE 2-3 days
Pre-immune Sera Collection 1 day
Immunization and Sera Collection (2 rabbits) 10 weeks
Immunization and Sera Collection (2 rabbits, fast) 6-8 weeks
ELISA Identification 2-3 days
Prescreen(ELISA) 2-3 days
Prescreen (Western Blot) 1 week
Prescreen (Dot Blot) 1 week
Additional Rabbit (1 rabbit) 6-10 weeks
Animal Maintenance (1 rabbit) 1 week
Additional Bleed (10 mL, 1 rabbit) 1 week
Extension Boost and Bleed (10 mL, 1 rabbit) 1 week
Immunization and Sera Collection (1 goat) 20 weeks
Immunization and Sera Collection (2 rats) 10 weeks
Immunization and Sera Collection (4 mice) 10 weeks
Immunization and Sera Collection (2 guinea pigs) 11 weeks

Polyclonal Antibody Production Packages

AAPPTec offers many Custom Polyclonal Antibody Production Packages to fill your requirements. The times listed are average times for delivery of custom polyclonal antibodies. Acutal delivery times may vary.

Package Options Service Items Delivery Time
Polyclonal Package 1 (From protein to antisera) Protein checked by SDS-PAGE, Immunization and Sera Collection, ELISA Identification 1-2 mL Pre-immune sera, 40-60 mL Antisera, ELISA result, Project report 10-12 weeks
Polyclonal Package 2 (From protein to antigen purified antibodies) Protein checked by SDS-PAGE, Immunization and Sera Collection, ELISA Identification 1-2 mL Pre-immune sera, 2-5 mg Purified antibodies, Elisa result, Project report 10-12 weeks
Polyclonal Package 3 (From peptide design to antisera) Peptide design and synthesis (12-22aa, 85%), Peptide conjugation, Immunization and sera collection, ELISA identification 2-3mg Peptides, 1-2ml Pre-immune sera, 40-60ml Antisera, ELISA result, Project report 12-15 weeks
Polyclonal Package 4 (From peptide design to antigen purified antibodies) Peptide design and synthesis (12-22aa, 85%), Peptide conjugation, Immunization and sera collection, ELISA identification, Antigen affinity purification 2-3 mg Peptides, 1-2 ml Pre-immune sera, 2-5 mg Purified antibodies, ELISA result, Project report 12-15 weeks
Polyclonal Package 5 (From plasmid to antisera) Protein expression and purification, Immunization and sera collection, ELISA identification 1 mg Protein, 1-2 ml Pre-immune sera, 40-60 ml Antisera, ELISA result, Project report 12-15 weeks
Polyclonal Package 6 (From plasmid to antigen purified antibodies) Protein expression and purification, Immunization and sera collection, ELISA identification, Antigen affinity purification 1 mg Protein, 1-2ml Pre-immune sera, 2-5 mg Purified antibodies, ELISA result, Project report 12-15 weeks
Polyclonal Package 7 (From cDNA to antisera) Gene amplification, Vector construction, Protein expression and purification, Immunization and sera collection, ELISA identification Recombinant vector, 1 mg Protein, 1-2 ml Pre-immune sera, 40-60 ml Antisera, ELISA result, Project report 14-16 weeks
Polyclonal Package 8 (From cDNA to antigen purified antibodies) Gene amplification, Vector construction, Protein expression and purification, Immunization and sera collection, ELISA identification, Antigen affinity purification, Antigen affinity purification Recombinant vector, 1 mg Protein, 1-2 ml Pre-immune sera, 2-5 mg purified antibodies, ELISA result, Project report 14-16 weeks
Polyclonal Package 9 (Phosphospecific polyclonal antibodies) Peptide design and synthesis (12-22aa, 85%), Peptide conjugation, Immunization and sera collection, ELISA identification, Antigen affinity purification 2-3 mg Peptides (unmodified and phospho), 1-2 ml Pre-immune sera, 2-5 mg Purified antibodies, ELISA result, Project report 13-16 weeks
Polyclonal Package 10 (Western guaranteed polyclonal antibodies) Peptide design and synthesis (12-22aa, 85%), Peptide conjugation, Immunization and sera collection, ELISA and Western blot identification, Antigen affinity purification 2-3 mg Peptides, 1-2 ml Pre-immune sera, 2-5 mg Purified antibodies, ELISA and Western blot results, Project report 15-18 weeks
Polyclonal Package 11 (Western and IHC guaranteed polyclonal antibodies) Peptide design and synthesis (12-22aa, 85%), Peptide conjugation, Immunization and sera collection, ELISA identification, Western blot and IHC identification, Antigen affinity purification 2-3 mg Peptides, 1-2 ml Pre-immune sera, 2-5 mg Purified antibodies, ELISA result, Westen blot and IHC results, Project report 15-18 weeks
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Chemistry and Materials
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Biochemistry & Molecular Biology
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Synthetic Chemistry
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Therapeutic Monoclonal Antibody (mAb) Development
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Biology
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Antibody Services
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Protein Services
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