2bind GmbH

Work with the experts in biophysics: 2bind GmbH, your provider of biophysical analytical services.
Our mission is to offers tailor-made biophysics services for drug discovery, antibody research, protein biophysics, and aptamer science and by that give researchers, developers, and decision makers the power to accelerate and streamline key research processes.

Our team of experts can help in high-throughput screening assays in order to identify potent drug canditates of protein, DNA, or RNA targets. In addition, our assays allow for characterization of lead candidates in terms of important binding parameters such as binding affinity, kinetics, and thermodynamics. Last but not least, we can assist with assays to understand the mode-of-action of a specific drug.

Our worldwide customers are mainly biotech and pharma companies as well as scientists from universities. Their main interest fields are drug discovery, drug development, antibody research, epigenetics, and aptamer development.

Up to date we have finished over 500 contract-research projects with more than 60 biotech companies, 6 of the top 10 pharma companies, as well as over 25 top-league universities.

Our assays are based on different technologies:

1. MicroScale Thermophoresis (MST)
   Our MST assays help in studying interactions between target biomolecules (proteins, DNA, RNA) and small molecule fragments, drug candidates, or drugs. We offer highly diverse assay types, e.g. library screenings, validation assays, or competition assays. Basic important binding parameters such as steady-state affinity, interaction stoichiometry, and thermodynamics can also be determined. MST is fast and precise, offers free choice of buffers or biological liquids, and features ultra-low sample consumption.

2. nanoDSF
   Our nanoDSF assays help in analyzing the thermal stability of target proteins as well as their thermal unfolding and potentially refolding. The nanoDSF method relies solely on the intrinsic fluorescence of tryptophan residues in the target proteins and thus does not required any labeling or immobilization and is a true in solution method. NanoDSF can also be used for analyzing binding of any kind of ligand to a target protein, by monitoring potential shifts in thermal stability by binding of the ligand. Buffer or detergent screens are also possible for determining the optimal conditions for the target protein in terms of stability. Furthermore, quality controls as well as ligand screens can be performed using this innovative technology. NanoDSF works without modification of the target protein, with free choice of buffer and low sample consumption

3. Biolayer Interferometry (BLI)
   Our BLI assay help in studying the kinetic features of biomolecular interactions (association rate, disscociation rate, kinetic affinity), but can also be modified to yield information on steady-state affinity. BLI is a surface based method, similar to surface plasmon resonance (SPR). The coupling chemistries are similar to that of SPR. BLI is very well suited for the study of antibody-interactions, because several immobilization methods exist that allow for a site-directed coupling of the antibodies.

4. Isothermal titration calorimetry (ITC)
   Our ITC assay help in studying the thermodynamics and stoichiometry of biomolecular interactions. ITC is a true in-solution method that does not require any labeling or immobilization. ITC assays are often used for analyzing a lower number of interactions at a high level of detail, including information on the stoichiometry of the interaction, the enthalpy and free enthalpy of binding, the steady-state affinity, as well as the interaction entropy.