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2bind GmbH

9 Orders Completed
Regensburg, DE

About 2bind GmbH

Work with the experts in biophysics: 2bind GmbH, your provider of biophysical analytical services. Our mission is to offers tailor-made biophysics services for drug discovery, antibody research, protein biophysics, and aptamer science and by that give researchers, developers, and decision makers the power to accelerate and streamline key research processes.

Benefit from our milestone based projects with clear cost structures, reliable timelines and personal project managers.

Our team of experts can help you with various biophysical approaches in 

1. Early Drug Discovery project:

At the Hit Identification stage

- HTS (150 K compound library)

- Fragment Screening (1920 fragments)

- In Silico Screening (2.7 Mio virtual compounds)

 

At the HIt Validation Stage

At the Hit Characterization Stage with information on

- Affinities

- Kinetics

- Thermodynamics

- Stoichiometry

- binding in bioliquids (serum, lysates)

 

2. Antibody development projects:

- Off rate Ranking

- Kinetic Characterization

- Affinity Maturation

- Pre-formulation

- Long term storage optimization

- Forced degradation

Up to date we have finished >500 contract-research projects with more than >100 customers (60 biotech companies, 7 of the top 10 pharma companies, as well as over 25 top-league universities).

2bind is the first service provider in the market certified by NanoTemper Technologies for MicroScale Thermophoresis (MST) and nanoDSF.

 

Our assays are based on different technologies:

1. MicroScale Thermophoresis (MST) Our MST assays help in studying interactions between target biomolecules (proteins, DNA, RNA) and small molecule fragments, drug candidates, or drugs. We offer highly diverse assay types, e.g. library screenings, validation assays, or competition assays. Basic important binding parameters such as steady-state affinity, interaction stoichiometry, and thermodynamics can also be determined. MST is fast and precise, offers free choice of buffers or biological liquids, and features ultra-low sample consumption.

2. nanoDSF Our nanoDSF assays help in analyzing the thermal stability of target proteins as well as their thermal unfolding and potentially refolding. The nanoDSF method relies solely on the intrinsic fluorescence of tryptophan residues in the target proteins and thus does not required any labeling or immobilization and is a true in solution method. NanoDSF can also be used for analyzing binding of any kind of ligand to a target protein, by monitoring potential shifts in thermal stability by binding of the ligand. Buffer or detergent screens are also possible for determining the optimal conditions for the target protein in terms of stability. Furthermore, quality controls as well as ligand screens can be performed using this innovative technology. NanoDSF works without modification of the target protein, with free choice of buffer and low sample consumption

3. Biolayer Interferometry (BLI) Our BLI assay help in studying the kinetic features of biomolecular interactions (association rate, disscociation rate, kinetic affinity), but can also be modified to yield information on steady-state affinity. BLI is a surface based method, similar to surface plasmon resonance (SPR). The coupling chemistries are similar to that of SPR. BLI is very well suited for the study of antibody-interactions, because several immobilization methods exist that allow for a site-directed coupling of the antibodies.

4. Isothermal titration calorimetry (ITC) Our ITC assay help in studying the thermodynamics and stoichiometry of biomolecular interactions. ITC is a true in-solution method that does not require any labeling or immobilization. ITC assays are often used for analyzing a lower number of interactions at a high level of detail, including information on the stoichiometry of the interaction, the enthalpy and free enthalpy of binding, the steady-state affinity, as well as the interaction entropy.

Our Services (12)


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Microscale Thermophoresis

Price on request

Our MST assays help in studying interactions between target biomolecules (proteins, DNA, RNA) and small molecule fragments, drug candidates, or drugs. We offer highly diverse assay types, e.g. library screenings, validation assays, or competition assays. Basic important binding parameters such as steady-state affinity, interaction stoichiometry, and thermodynamics can also be determined. MST is fast and precise, offers free choice of buffers or biological liquids, and features ultra-low sample consumption.

Monolith NT.115 pico Monolith NT.115 Monolith NT.LabelFree Monolith NT.Automated Labcyte Echo 525 Automated liquid handler Drug Discovery MicroScale Thermophoresis Proteins Peptides Small molecules DNA RNA Aptamers Antibodies Monoclonal antibodies Show 16 more tags Show less

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NanoDSF

Price on request

Our nanoDSF assays help in analyzing the thermal stability of target proteins as well as their thermal unfolding and potentially refolding. The nanoDSF method relies solely on the intrinsic fluorescence of tryptophan residues in the target proteins and thus does not required any labeling or immobilization and is a true in solution method. NanoDSF can also be used for analyzing binding of any kind of ligand to a target protein, by monitoring potential shifts in thermal stability by binding of the ligand. Buffer or detergent screens are also possible for determining the optimal conditions for the target protein in terms of stability. Furthermore, quality controls as well as ligand screens can be performed using this innovative technology. NanoDSF works without modification of the target protein, with free choice of buffer and low sample consumption

Prometheus NT.48 Drug Discovery Differential Scanning Fluorimetry nanoDSF Prometheus NT.48 Proteins Small molecules Peptides Labcyte Echo 525 Antibodies Monoclonal antibodies Show 11 more tags Show less

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Isothermal Titration Calorimetry (ITC)

Price on request

Our ITC assay help in studying the thermodynamics and stoichiometry of biomolecular interactions. ITC is a true in-solution method that does not require any labeling or immobilization. ITC assays are often used for analyzing a lower number of interactions at a high level of detail, including information on the stoichiometry of the interaction, the enthalpy and free enthalpy of binding, the steady-state affinity, as well as the interaction entropy.

Malvern MicroCal PEAQ-ITC Drug Discovery Isothermal Titration Calorimetry Malvern MicroCal PEAQ-ITC Proteins Peptides Antibodies Monoclonal antibodies Small molecules DNA RNA Show 11 more tags Show less

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Biolayer Interferometry

Price on request

Our BLI assay help in studying the kinetic features of biomolecular interactions (association rate, disscociation rate, kinetic affinity), but can also be modified to yield information on steady-state affinity. BLI is a surface based method, similar to surface plasmon resonance (SPR). The coupling chemistries are similar to that of SPR. BLI is very well suited for the study of antibody-interactions, because several immobilization methods exist that allow for a site-directed coupling of the antibodies.

ForteBio Octet Drug Discovery Biolayer Interferometry ForteBio Octet Proteins Peptides DNA RNA Aptamers Small molecules Show 10 more tags Show less

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Chemistry and Materials

Price on request
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Imaging & Spectroscopy

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Interferometry

Price on request
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Analytical Chemistry Services

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Engineering and Fabrication

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Physical Analysis Methods

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Calorimetry

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Thermal Analysis Services

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  • Positive review for NanoDSF:

    July 2019

    "Extremely professional and clear communication, robust and clear data analysis/interpretation, affordable, and very quick services. Quite knowledgeable in DSF and MST as they greatly aided in experimental design. We are currently pursuing additional experiments together. Highly recommend!"

  • Positive review for Calorimetry:

    April 2019

    "2bind has an amazing team of professionals who work to the highest standards."

2bind GmbH has not received any endorsements.